Phytoactinopolyspora mesophila sp. nov., isolated from a saline–alkaline soil

Abstract

A novel moderately halophilic, filamentous actinobacterium, designated as XMNu-373 T , was isolated from a saline-alkaline soil sample collected from the Mongolia Plateau, Dongwu County, Inner Mongolia Autonomous Region, PR China. The isolate grew optimally at 28-37 °C, pH 7.0-8.0 and with 2-5 % (w/v) NaCl. The substrate mycelia fragmented into rod-like elements, and the white aerial mycelia formed spore chains at maturity. The predominant menaquinone was MK-9(H 4 ). The polar lipids were diphosphatidylglycerol, three unidentified phosphoglycolipids, an unidentified aminophospholipid, two phosphatidylinositol mannosides, four unidentified phospholipids, phosphatidylglycerol and two unidentified lipids. The major cellular fatty acids were iso-C 16 : 0 , anteiso-C 17 : 0 and anteiso-C 15 : 0 . The genomic DNA G+C content was 66.2 mol%. It shared high 16S rRNA gene sequence similarities to Phytoactinopolyspora halotolerans YIM 96448 T (96.1 %) and Phytoactinopolyspora endophytica EGI 60009 T (96.0 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain XMNu-373 T resided in the clade of family Jiangellaceae, and it formed a monophyletic branch distinct from four other recognized type species in the subclade of the genus Phytoactinopolyspora. On the basis of polyphasic taxonomic evidence, strain XMNu-373 T represents a novel species of the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora mesophila sp. nov. is proposed. The type strain is XMNu-373 T (=JCM 33740 T =CGMCC 4.7654 T ).

Aminipila luticellarii sp. nov., an anaerobic bacterium isolated from the pit mud of strong aromatic Chinese liquor, and emended description of the genus Aminipila

“…The 16S rRNA genes of type strains which share the highest similarity to strain JN-18 T were downloaded and used to reconstruct phylogenetic trees with the neighbour-joining, maximum-likelihood and minimum-evolution methods using the mega version 7.0 software package, with 1000 bootstrap replicates [18–21]. OrthoANI (www.ezbiocloud.net/tools/ani) was used to calculate the average nucleotide identity (ANI) between strains JN-18 T and DSM 103574 T , average amino acid identity (AAI) was calculated using Compare M (https://github.com/dparks1134/CompareM), and the Genome-to-Genome Distance Calculator 2.1 (http://ggdc.dsmz.de/ggdc.php) was used to calculate the digital DNA–DNA hybridization (dDDH) value for them [22, 23]. Both the genome and 16S rRNA gene (1506 nt) of JN-18 T were submitted to GenBank.…”

“…de/ ggdc. php) was used to calculate the digital DNA-DNA hybridization (dDDH) value for them [22,23]. Both the genome and 16S rRNA gene (1506 nt) of JN-18 T were submitted to GenBank.…”

Rare Halophilic Nocardiopsis from Algerian Saharan Soils as Tools for Biotechnological Processes in Pharmaceutical Industry

“…Hypersaline ecosystems represent an extreme environment in which a relatively low diversity of microbial species can be found and host a particular native microflora adapted to these habitats such as halophilic bacteria [ 1 ]. The occurrence of actinobacteria in high salinity environments and tolerance of these organisms to high concentrations of salt have been previously studied by many authors, and several novel genera and species of halophilic and halotolerant have been described such as Streptomonospora litoralis [ 2 ], Phytoactinopolyspora halophila [ 3 ], P. mesophila [ 4 ], Glycomyces salinus [ 5 ], Nesterenkonia pannonica [ 6 ], N. natronophila [ 7 ], Prauserella isguenensis [ 8 ], P. oleivorans [ 9 ], Mzabimyces algeriensis [ 10 ], Actinopolyspora salinaria [ 11 ], and Brachybacterium halotolerans [ 12 ]. Halophiles and halotolerant actinobacteria have attracted a great attention owing to produce various bioactive natural compounds, such as antibiotics actinopolysporins A-C [ 13 ], persiamycin A [ 14 ], anticancer salternamide A [ 15 ], cytotoxic compounds nocarbenzoxazoles A-G [ 16 ], salternamides A-D [ 17 ], antiviral xiamycins C-E [ 18 ], and enzymes [ 19 ].…”