Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway

Lu, Chunni; Yuan, Zihao; Zhang, Xiaoli; Yan, Ru; Zhao, Yaqin; Liao, Ming; Chen, Jianxin

doi:10.1016/j.intimp.2012.06.010

Cited by 155 publications

(106 citation statements)

References 32 publications

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“…These observations coupled with the development of the reddish color in the LiebermannBurchard test [31] (data not shown), as well as the occurrence of triterpenes in the Byrsonima genus [13,14,32]. Lu et al [33] and An et al [34] demonstrated that triterpene glycosides, isolated from two species of the genus Bupleurum, have in vitro anti-inflammatory activity via the inhibition of the release of NO and PGE 2 (prostaglandin E 2 ).…”

Section: Compounds Identificationmentioning

confidence: 94%

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

et al. 2016

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An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography-mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

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Section: Compounds Identificationmentioning

confidence: 94%

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

et al. 2016

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“…Saikosaponin A and saikosaponin D significantly repressed inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression, reduced TNF-ɑ and IL-6 production, and inhibited NF-κB translocation in lipopolysaccharide (LPS)-induced murine macrophage cell line RAW264.7 cells (91). In vitro study revealed that saikosaponin A suppressed osteoclastogenesis in C57/BL6 mice bone marrow monocytes and mediated osteoclast differentiation through inhibiting RANKL-induced p38, ERK, JNK, and NF-κB activation in murine RAW264.7 cell line (92).…”

Section: Saikosaponinsmentioning

confidence: 99%

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Lin

Wang

Zhang

et al. 2017

BST

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“…Saikosaponin-d (SSd), an extract from a traditional Chinese herb, Bupleurum chinensis DC, exhibits various pharmacological properties, such as anti-inflammation [14,15], hepatocyte injury prevention [16], angiogenesis inhibition [17], and anti-cancer [18,19]. In our preliminary experiments, SSd and radiation are administered to treat liver cancer; the results have revealed that this combined treatment is more effective than either of these treatments administered alone.…”

Section: Introductionmentioning

confidence: 99%

Saikosaponin-D Enhances Radiosensitivity of Hepatoma Cells under Hypoxic Conditions by Inhibiting Hypoxia-Inducible Factor-1a

Wang

Kang

et al. 2014

Cell Physiol Biochem

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Background: Our previous study revealed that the combination of Saikosaponin-d (SSd) and radiation is more effective in the treatment of liver cancer than the application of either of these monotherapeutic methods. However, the molecular mechanisms of the radiosensitizing effect of SSd on liver cancer remained ill defined. Methods: Cells were treated with different interventions; afterward, cell viability, apoptosis, and cell survival of SMMC-7721 and HepG2 hepatoma cells were examined. Xenograft tumor models were established by subcutaneously injecting SMMC-7721 cells. The molecular mechanism was assessed by western blot. Results: SSd dose-dependently increased radiosensitivity of hepatoma cells under hypoxic condition. The growth inhibitory effect of the combined treatment was correlated with cell apoptosis. Further mechanistic analysis indicated that SSd induced the upregulation of p53 and Bax as well as the downregulation of Bcl-2 by attenuating HIF-1α expression under hypoxic condition. These effects were enhanced when the HIF-1α inhibitor PX-478 was introduced. In vivo data also presented a more significant suppression of tumor xenograft growth from the combined therapy than from either of the monotherapeutic methods. Conclusions: Our study provides evidence for a radiosensitizing effect of SSd on hepatoma cells under hypoxic conditions by inhibiting HIF-1α expression. Thus, SSd can be used as a potential sensitizer in hepatoma radiotherapy.

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Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway

Cited by 155 publications

References 32 publications

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Saikosaponin-D Enhances Radiosensitivity of Hepatoma Cells under Hypoxic Conditions by Inhibiting Hypoxia-Inducible Factor-1a

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