Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Abstract

An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography-mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

“…exhibited significant anti-edematogenic activity in both phases. According to the literature, in the some studies of the pharmacological effects of natural products, using these models and the intraperitoneal route, the treatments with plant extracts showed antiinflammatory and antinociceptive activities at higher doses (75-500 mg.kg -1 ) (ALMEIDA et al, 2012;IBRAHIM et al, 2002;NARDI et al, 2003;SADANHA et al, 2016) than those used for FHMeOH in the present study.…”

Dereplication by HPLC‐DAD‐ESI‐MS/MS and Screening for Biological Activities of Byrsonima Species (Malpighiaceae)

“…Saldanha et al . () demonstrated that the MeOH extract of B. verbascifolia inhibited NO production by macrophages induced with LPS and attributed these effects to a combination of compounds present in the extract, including catechins, flavonoids and triterpene glycosides. Moreover, in vivo , those extracts were able to inhibit acute swelling using two different models of acute inflammation induced by carrageenan, paw edema and experimental pleurisy (Saldanha et al ., ).…”

“…Moreira et al (2011) reported that the aqueous extract of B. intermedia leaves exhibited potent anti-inflammatory activity in animal models by reducing carrageenan-induced paw edema and granuloma formation in a chronic inflammation model; this activity was attributed to the presence of catechin and flavonoids. Saldanha et al (2016b) demonstrated that the MeOH extract of B. verbascifolia inhibited NO production by macrophages induced with LPS and attributed these effects to a combination of compounds present in the extract, including catechins, flavonoids and triterpene glycosides. Moreover, in vivo, those extracts were able to inhibit acute swelling using two different models of acute inflammation induced by carrageenan, paw edema and experimental pleurisy (Saldanha et al, 2016b).…”

“…Studies of the genus Byrsonima focus mainly on antimicrobial activity against a range of bacteria, enterobacteria, mycobacteria, protozoa and fungi related to gastrointestinal and skin diseases (Guilhon-Simplicio and Pereira, 2011). In last few years, some studies on other biological properties have been reported in different species and parts of plants, such as antioxidant activity (Gordon et al, 2011;Mariutti et al, 2014;Vargas et al, 2016), central nervous system depressant activity (Herrera-Ruiz et al, 2011), anti-diabetes activity (Perez-Gutierrez et al, 2010, Pérez-Gutierrez andRamirez, 2016), and anti-inflammatory activity (Maldini et al, 2009;Moreira et al, 2011;Saldanha et al, 2016a;Saldanha et al, 2016b).…”

“…Promising results regarding the ethnopharmacological use of some species has motivated research into their chemical composition and biological activities. Most studies have dealt with the fruits and leaves of B. crassa and B. crassifolia, with few reports of other plants (Malta et al, 2013;Mariutti et al, 2014;Pérez-Gutiérrez and Ramirez, 2016;Saldanha et al, 2016a;Saldanha et al, 2016b). The present work reports the metabolic fingerprinting of methanol (MeOH) and ethyl acetate (EtOAc) extracts of four different Byrsonima species from the Brazilian Cerrado by high-performance liquid chromatography with diode array detector coupled to electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MS/MS), as well as evaluation of their antioxidant, antiinflammatory, anti-glycation and cytotoxic activities.…”