Safety evaluation of Streptomyces murinus glucose isomerase

Enzymes in cleaning products: An overview of toxicological properties and risk assessment/management

“…Note that these have involved, on some occasions, use of appropriately modified assays, since the methods are designed for chemicals rather than proteins but tests are nevertheless carried out, driven largely by regulatory requirements. The results reflect the outcome that would be predicted based on intrinsic properties of enzymes and given appropriate methods of production (Pariza and Foster, 1983;Ashby et al, 1987;Greenough et al, 1991Greenough et al, , 1996Hjortkjaer et al, 1986Hjortkjaer et al, , 1993Pariza and Johnson, 2001;van Dijck et al, 2003;REACH, 2010a,b). Importantly, these tests also ensure that any residual materials from the production fermentation are also free from genotoxic effects.…”

Glucose Transforming Enzymes

“…The preparations are not allowed to be mutagenic, nor to provoke chromosomal changes. They should not contain antimicrobial and teratogenic activity (Ashby et al, 1987). In feeding and injection tests no signs of infections or toxicosis are allowed (Porter et al, 1984).…”

Two-Step Production of Neofructo-Oligosaccharides Using Immobilized Heterologous Aspergillus terreus 1F-Fructosyltransferase Expressed in Kluyveromyces lactis and Native Xanthophyllomyces dendrorhous G6-Fructosyltransferase

“…5.3.1.5) are D-xylose-ketol-isomerases that convert glucose into fructose, which also reduces of the glycemic index of the solution. The glucose isomerase from Streptomyces murinus is suitable for industrial applications because it is neither mutagenic nor teratogenic [22] and is widely used for the production of high-fructose sugar syrup [23]. Optimal isomerase activity occurs in the presence of 1 M glucose and 5 mM Mg 2+ as a cofactor, at a temperature of 60 • C and a pH of 7.5 [24].…”