Safety evaluation of proanthocyanidin polymer-rich fraction obtained from stem bark of Stryphnodendron adstringens (BARBATIMÃO) for use as a pharmacological agent

Costa, Marco A. Dalla; Ishida, Kelly; Kaplum, Vanessa; Koslyk, Éryca Danielle Alves; Mello, João Carlos Palazzo de; Ueda-Nakamura, Tânia; Filho, Benedito Prado Dias

doi:10.1016/j.yrtph.2010.07.006

Cited by 33 publications

(30 citation statements)

References 43 publications

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“…These results further confirm that the extracts were antigenotoxic. Our results are in agreement with Costa et al (2010) who observed no genotoxic effect of the proanthocyanidin polymerrich fraction of stem bark of S. adstringens. This work reported no significant differences in the frequency of MNPCE induced by the fraction treatment when compared to the negative control.…”

Section: Resultssupporting

confidence: 94%

Protective action against chemical-induced genotoxicity and free radical scavenging activities of Stryphnodendron adstringens ("barbatimão") leaf extracts

Filho¹,

Ferreira²,

Gouvêa³

2011

Rev. bras. farmacogn.

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The present study describes for the first time the antigenotoxic and antioxidant properties of Stryphnodendron adstringens (Mart.) Coville, Fabaceae ("barbatimão") leaves. The aqueous (AEB), water fraction (WFB) and ethanolic (EEB) extracts of leaves were obtained and they presented high content of phenolic compounds, flavonoids and proanthocyanidins. WFB and EEB inhibited the genotoxicity induced by cyclophosphamide. WFB inhibited the DNA lesion formation and both WFB and EEB decreased significantly (p<0.05) micronucleus formation. All extracts also showed high DPPH radical scavenging activity and reducing power. In conclusion all extracts presented antioxidant activity and WFB and EEB protected cells from genotoxicity induced by cyclophosphamide in rat bone marrow cells. Thus, our results support the beneficial effects of the barbatimão extracts as an anticarcinogenic agent.

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Section: Resultssupporting

confidence: 94%

Protective action against chemical-induced genotoxicity and free radical scavenging activities of Stryphnodendron adstringens ("barbatimão") leaf extracts

Filho¹,

Ferreira²,

Gouvêa³

2011

Rev. bras. farmacogn.

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“…For this purpose, the genotoxicity of parthenolide in a mouse model was determined using a micronucleus test and cyclophosphamide as the positive control because it is a known genotoxin [25]. Micronuclei are masses of cytoplasmic chromatin that appear outside the main nucleus as a result of chromosomal damage or damage to the mitotic apparatus in the erythroblasts of the test species, and they can be used as an indicator of the effects of agents that cause DNA damage [26]. In mice, micronuclei in mature erythrocytes in peripheral blood live approximately 1 month, providing a measure of average chromosomal damage [27].…”

Section: Discussionmentioning

confidence: 99%

Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide

2014

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BackgroundLeishmania amazonensis infection results in diverse clinical manifestations: cutaneous, mucocutaneous or visceral leishmaniasis. The arsenal of drugs available for treating Leishmania infections is limited. Therefore, new, effective, and less toxic leishmaniasis treatments are still needed. We verified cell death in amastigote forms of Leishmania amazonensis induced by the sesquiterpene lactone parthenolide.ResultsThe tested compound was able to concentration-dependently affect axenic and intracellular amastigotes, with IC50 values of 1.3 μM and 2.9 μM, respectively after 72 h incubation. No genotoxic effects were observed in a micronucleus test in mice. Parthenolide induced morphological and ultrastructural changes in axenic amastigotes, including a loss of membrane integrity, swelling of the mitochondrion, cytoplasmic vacuoles, and intense exocytic activity in the region of the flagellar pocket. These results led us to investigate the occurrence of autophagic vacuoles with monodansylcadaverine and the integrity of the plasma membrane and mitochondrial membrane potential using flow cytometry. In all of the tests, parthenolide had positive results.ConclusionsOur results indicate that the antileishmanial action of parthenolide is associated with autophagic vacuole appearance, a reduction of fluidity, a loss of membrane integrity, and mitochondrial dysfunction. Considering the limited repertoire of existing antileishmanial compounds, the products derived from medicinal plants has been one the greatest advances to help develop new chemotherapeutic approaches.

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“…The existence of micronucleated polychromatic erythrocytes was determined by counting 2000 red cells. The mutagenic effect was characterized by a significant increase in micronucleated polychromatic erythrocytes compared to the negative control (Costa et al, 2010;Schmid, 1975;Vilar et al, 2008).…”

Section: Mutagenicitymentioning

confidence: 99%

Acute toxicity and long-term safety evaluation of the crude extract from rhizomes of Limonium brasiliense in mice and rats

Antonelli-Ushirobira

Blainski

Fernandes

et al. 2015

Journal of Ethnopharmacology

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Safety evaluation of proanthocyanidin polymer-rich fraction obtained from stem bark of Stryphnodendron adstringens (BARBATIMÃO) for use as a pharmacological agent

Cited by 33 publications

References 43 publications

Protective action against chemical-induced genotoxicity and free radical scavenging activities of Stryphnodendron adstringens ("barbatimão") leaf extracts

Protective action against chemical-induced genotoxicity and free radical scavenging activities of Stryphnodendron adstringens ("barbatimão") leaf extracts

Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide

Acute toxicity and long-term safety evaluation of the crude extract from rhizomes of Limonium brasiliense in mice and rats

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