Safety, efficacy and anti-inflammatory activity of rho iso-alpha-acids from hops

Hall, Amy; Babish, John G.; Darland, Gary; Carroll, Brian; Konda, Veera Reedy; Lerman, Robert H.; Bland, J.S.; Tripp, Matthew L.

doi:10.1016/j.phytochem.2008.02.001

Cited by 46 publications

(51 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were subcultured in 96-well plates and test compounds pre-incubated for 1 h in serum-free media, followed by 4 h of LPS stimulation (1 μg/ml). PGE 2 in the media was then measured (Assay Designs, Ann Arbor, MI) as described [11]. For NO production, media were collected after 16-20 h of LPS stimulation and nitrate/nitrite levels measured (Cayman Chemicals).…”

Section: Cell Culture and Stimulationmentioning

confidence: 99%

“…Western blot analysis of COX-2 RAW 264.7 cells were pre-incubated with test compounds for 1 h and stimulated with LPS (1 μg/ml) for 16 h. Cells were washed with PBS and lysed samples were analyzed by western blot with COX-2 antibody as described [11].…”

Section: Cell Culture and Stimulationmentioning

confidence: 99%

“…We previously reported the anti-inflammatory role of rho-iso-alpha acids (RIAA) based on the inhibition of LPS-induced PGE 2 production and COX-2 expression in RAW 264.7 cells [11]. In addition, a defined RIAA preparation was found clinically to be effective in reducing knee arthritic pain [11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

META060 inhibits multiple kinases in the NF-κB pathway and suppresses LPS – mediated inflammation in vitro and ex vivo

Desai¹,

Konda²,

Darland³

et al. 2009

Inflamm. Res.

View full text Add to dashboard Cite

show abstract

Section: Cell Culture and Stimulationmentioning

confidence: 99%

Section: Cell Culture and Stimulationmentioning

confidence: 99%

See 1 more Smart Citation

META060 inhibits multiple kinases in the NF-κB pathway and suppresses LPS – mediated inflammation in vitro and ex vivo

Desai¹,

Konda²,

Darland³

et al. 2009

Inflamm. Res.

View full text Add to dashboard Cite

show abstract

“…1. These photolytic protective properties allows for the use of these products to be commonly used in isolation in unprotected clear glass bottles [2,4,7,8]. However in order to stablise the bitterness of the beer, they may be used also in conjunction with traditional IAA hopping techniques in green bottles that are subject to low levels of light where some photolytic degradation will still occur to the natural IAA [9].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of reduced iso-α-acids in volunteers following clear bottled beer consumption

Rodda

Gerostamoulos

Drummer

2015

Forensic Science International

View full text Add to dashboard Cite

“…Tetrahydro-iso-α-acids are obtained by hydrogenation of the double bonds in the side chains of the iso-α-acid and hexahydro-iso-α-acids are accessible by a combination of the reduction of the side-chain carbonyl group and hydrogenation of the double bonds (De Keukeleire, 2000). Iso-α and reduced iso-α-acid contribute to bittering values and antimicrobial property in beer and are easily deprotonated, being commonly referred to as acids, due to their beta triketo moiety (Garcia-Villalba et al, 2006;Hall et al, 2008), having pronounced bacteriostatic activity; they strongly inhibit the growth of Gram-positive bacteria (De Keukeleire, 2000).…”

Section: Introductionmentioning

confidence: 99%

Bitter acids from hydroethanolic extracts of Humulus lupulus L., Cannabaceae, used as anxiolytic

Negri¹,

Santi²,

Tabach³

2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO:Humulus lupulus L., Cannabaceae, é usada como sedativo e ansiolítico na medicina popular. O método de HPLC-DAD-ESI-MS n representa uma ferramenta poderosa para a análise de produtos naturais, desde que ela fornece o espectro de UV e informações estruturais sobre os constituintes da mistura. O objetivo deste trabalho foi o de caracterizar os constituintes encontrados no extrato hidroalcoólico. Os constituintes 1-9 foram tentativamente caracterizados através do UV/DAD e ionização por electrospray (MS/MS) depois da separação usando fase reversa, tempo de retenção e dados da literatura. Os principais compostos fenólicos (baseados na área dos picos) foram caracterizados como ácido hulupínico (9), coulupona (8), dois alfaácidos amargos oxidados (principais constituintes), um deles sendo um derivado da coumulinona oxidada (5) e o outro um derivado da humulinona oxidada (7), junto com uma procianidina B (3) e os flavonoides rutina (4) e o canferol-7-O-rutinosídeo (6). Esta planta conhecida devido às suas propriedades ansiolíticas e por ser um componente da cerveja, mostrou derivados oxidados de alfa-ácidos, como principais constituintes do extrato hidroalcoólico. Unitermos: Humulus lupulus, flavonoides glicosídeos, derivados de alfa-ácidos amargos oxidados, HPLC/DAD/MS/MS.ABSTRACT: Humulus lupulus L., Cannabaceae, is commonly used as light sedative and anxiolytics in folk medicine. HPLC-DAD-ESI-MS n represents a powerful tool for the analysis of natural products, since it can simultaneously provide a UV chromatogram and significant structural information about compounds in complex mixture. The aim of this work was characterize the constituents present in hydroethanolic extract. Compounds 1-9 were tentatively characterized on the basis of UV, MS/MS, after reversed phase separation, retention time and literature data. The main phenolic compounds (based on peak area) were characterized as hulupinic acid (9), cohulupone (8), two oxidized hop alfa-bitter acids (principal constituents), one being a oxidized cohumulinone (5) and the other an oxidized humulinone (7) derivatives, together with a procyanidin dimer B (3), flavonoids rutin (4) and kaempferol-7-O-rutinoside (6). This plant known, due to anxiolytic property and beer flavoring, showed oxidized hop bitter acids, as principal constituents, in its hydroethanolic extract.

show abstract

Safety, efficacy and anti-inflammatory activity of rho iso-alpha-acids from hops

Cited by 46 publications

References 40 publications

META060 inhibits multiple kinases in the NF-κB pathway and suppresses LPS – mediated inflammation in vitro and ex vivo

META060 inhibits multiple kinases in the NF-κB pathway and suppresses LPS – mediated inflammation in vitro and ex vivo

Pharmacokinetics of reduced iso-α-acids in volunteers following clear bottled beer consumption

Bitter acids from hydroethanolic extracts of Humulus lupulus L., Cannabaceae, used as anxiolytic

Contact Info

Product

Resources

About