Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance

Petrezsélyová, Silvia; Zahrádka, Jaromír; Sychrová, Hana

doi:10.1016/j.funbio.2009.11.002

Cited by 58 publications

(42 citation statements)

References 24 publications

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“…Gene disruptions were confirmed by sequencing PCR products of the regions flanking the kanMX insertion. The BY4741 trk1 trk2 mutant strain was kindly provided by Dr. Hana Sychrová (52). The BY4741 hal4 hal5 strain has been described previously (4,6).…”

Section: Methodsmentioning

confidence: 99%

Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation

Primo

Ferri-Blázquez

Loewith

et al. 2017

Journal of Biological Chemistry

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Edited by Thomas SöllnerThe proper maintenance of potassium homeostasis is crucial for cell viability. Among the major determinants of potassium uptake in the model organism Saccharomyces cerevisiae are the Trk1 high affinity potassium transporter and the functionally redundant Hal4 (Sat4) and Hal5 protein kinases. These kinases are required for the plasma membrane accumulation of not only Trk1 but also several nutrient permeases. Here, we show that overexpression of the target of rapamycin complex 1 (TORC1) effector NPR1 improves hal4 hal5 growth defects by stabilizing nutrient permeases at the plasma membrane. We subsequently found that internal potassium levels and TORC1 activity are linked. Specifically, growth under limiting potassium alters the activities of Npr1 and another TORC1 effector kinase, Sch9; hal4 hal5 and trk1 trk2 mutants display hypersensitivity to rapamycin, and reciprocally, TORC1 inhibition reduces potassium accumulation. Our results demonstrate that in addition to carbon and nitrogen, TORC1 also responds to and regulates potassium fluxes.

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Section: Methodsmentioning

confidence: 99%

Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation

Primo

Ferri-Blázquez

Loewith

et al. 2017

Journal of Biological Chemistry

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“…The trk1 (BYT1), trk2 (BYT2), and trk1 trk2 (BYT12) strains were constructed using the Cre-loxP system (Guldener et al 1996) as described by Petrezsélyová et al (2010). Strains were routinely grown at 30°C either in rich (YPD) or minimal (SD) media solidified with 2% agar when necessary.…”

Section: Methodsmentioning

confidence: 99%

Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiae

2011

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In Saccharomyces cerevisiae, TRK1 and TRK2 genes encode partially redundant K(+) transporters. Direct involvement in K(+) uptake has been shown for Trk1p since cells growing under limiting environmental K(+) concentrations demand its presence. The biological role of Trk2p is less understood. In our experiments, TRK2 overexpression improved the ability of trk1 cells to grow in low K(+) and led to a higher accumulation of K(+). Using diS-C(3)(3) as a potentiometric probe, we revealed a higher hyperpolarization of trk2 cells compared to the wild type. In addition, the deletion of TRK2 in the trk1 genetic background increased the cell sensitivity to hygromycin B, spermine, and TMA. Our studies reinforced the conclusion that Trk1p is the prominent K(+) uptake transporter and for the first time revealed that though Trk2p is much less effective, its activity contributes significantly to K(+) supply and the maintenance of plasma-membrane potential.

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“…Primer combinations are listed in Supplemental Table S3. The constructs were transformed in the yeast strain BY4741 (Brachmann et al, 1998), BYT12 (trk1D trk2D; Petrezselyova et al, 2010), or BYT45 (ena1-5D nha1D; Navarrete et al, 2010) by the lithium acetate method (Ito et al, 1983). For each combination of two plasmids, two independent double transformants were generated to confirm identical growth properties.…”

Section: Yeast Growth Assaysmentioning

confidence: 99%

“…The next day, the cells were washed with deionized water and resuspended to an optical density at 600 nm wavelength (OD 600 ) value of 1, and 10-fold serial dilutions from OD 600 of 1 to 10 25 were prepared; 20 mL of each dilution was dropped on YNB agar plates supplemented with or without 1 M KCl for export or import experiments, respectively. The HygB sensitivity test was performed according to Petrezselyova et al, 2010; 3 mL of cell suspension (OD 600 = 1) was spotted on HygB gradient plates consisting of YNB medium supplemented with 100 mM KCl to support growth of mutant BYT12.…”

Section: Yeast Growth Assaysmentioning

confidence: 99%

Nitrate-dependent control of shoot K homeostasis by NPF7.3/NRT1.5 and SKOR in Arabidopsis

et al. 2015

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Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance

Cited by 58 publications

References 24 publications

Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation

Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation

Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiae

Nitrate-dependent control of shoot K homeostasis by NPF7.3/NRT1.5 and SKOR in Arabidopsis

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