Nitrate-dependent control of shoot K homeostasis by NPF7.3/NRT1.5 and SKOR in Arabidopsis

Drechsler, Navina; Zheng, Yue; Bohner, Anne; Nobmann, Barbara; Wirén, Nicolaus von; Kunze, Reinhard; Rausch, Christine

doi:10.1104/pp.15.01152

Cited by 64 publications

(65 citation statements)

References 69 publications

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“…6 We recently found that NRT1.5 is also involved in potassium (K C ) homeostasis in shoots by affecting K C root-to-shoot translocation in a NO 3 ¡ dependent manner. 7 Lately the same phenomenon was reported in a different study. 8 Lack of NRT1.5 in roots results in K C deficiency in shoots under low NO 3 ¡ nutrition, whereas the whole root elemental composition remains unchanged.…”

Section: Introductionsupporting

confidence: 64%

“…1A). 7 Shoot and root fresh weight and root architecture of wild-type and nrt1.5 mutants are virtually identical though ( Fig. 1A-C).…”

Section: Introductionmentioning

confidence: 86%

“…20 Drechsler et al recently discovered that nrt1.5 mutant plants, when growing under low N fertilization conditions, exhibit a pleiotropic shoot phenotype and early leaf senescence symptoms, that are caused by K C deficiency in the shoot organs. 7 However, the NRT1.5 gene is mainly expressed in root pericycle cells which surround the xylem. 6 This prompted us to investigate root development of nrt1.5 mutants under varying NO 3 ¡ and K C supply.…”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation

Zheng

Drechsler

Rausch

et al. 2016

Plant Signaling & Behavior

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Section: Introductionsupporting

confidence: 64%

“…1A). 7 Shoot and root fresh weight and root architecture of wild-type and nrt1.5 mutants are virtually identical though ( Fig. 1A-C).…”

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation

Zheng

Drechsler

Rausch

et al. 2016

Plant Signaling & Behavior

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“…qPCR reactions were run on an ABI 7500 Fast RealTime PCR System (Thermo Fisher Scientific, USA) using the Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, USA) and the thermal profile: 3 min 95°C (30 s 95°C, and 40 cycles of 95°C for 30 s, 60°C for 1 min and 72°C for 30 s). For MS-grown plants, RNA extraction, cDNA synthesis and qPCR were performed as described by Drechsler et al (2015). In both experiments, the absence of genomic DNA was tested by qPCR using primers designed to amplify an intergenic region as described by Caldana et al (2007) and the quality of cDNA synthesis was estimated by qPCR using 5′ and 3′ specific elongation factor 1α (EF1α; Os03g08020) primer pairs (Supplementary Table T3).…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%

“…The nitrate transporter 1/peptide transporter family (NPF) is a large protein family whose members transport not only NO 3 − with low-affinity in diverse tissues and at different developmental stages (Krouk et al 2010, and references therein), but also di-/tripeptides, amino acids, nitrite, glucosinolates, or phytohormones (reviewed in Leran et al 2014;and Fan et al 2017). Recently, the Arabidopsis NPF7.3/NRT1.5 was shown to be a K + transporter and to NO 3 − -dependently control K + homeostasis in shoots (Drechsler et al 2015;Li et al 2017). Other family members, for example, function as a NO 3 − excretion transporter or in actinorhizal and rhizobial N-fixing symbioses (Jeong et al 2004;Segonzac et al 2007;Yendrek et al 2010), whereas a role of NPF proteins in plant-AMF interactions has not been observed yet.…”

Section: Introductionmentioning

confidence: 99%

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

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Arbuscular mycorrhizal fungi (AMF) colonize up to 90% of all land plants and facilitate the acquisition of mineral nutrients by their hosts. Inorganic orthophosphate (P i ) and nitrogen (N) are the major nutrients transferred from the fungi to plants. While plant P i transporters involved in nutrient transfer at the plant-fungal interface have been well studied, the plant N transporters participating in this process are largely unknown except for some ammonium transporters (AMT) specifically assigned to arbuscule-colonized cortical cells. In plants, many nitrate transporter 1/peptide transporter family (NPF) members are involved in the translocation of nitrogenous compounds including nitrate, amino acids, peptides and plant hormones. Whether NPF members respond to AMF colonization, however, is not yet known. Here, we investigated the transcriptional regulation of 82 rice (Oryza sativa) NPF genes in response to colonization by the AMF Rhizophagus irregularis in roots of plants grown under five different nutrition regimes. Expression of the four OsNPF genes NPF2.2/ PTR2, NPF1.3, NPF6.4 and NPF4.12 was strongly induced in mycorrhizal roots and depended on the composition of the fertilizer solution, nominating them as interesting candidates for nutrient signaling and exchange processes at the plantfungal interface.

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Comparative physiological, transcriptome, and qRT‐PCR analysis provide insights into osmotic adjustment in the licorice species Glycyrrhiza inflata under salt stress

Cai

Lin

et al. 2023

Crop Science

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Licorice (Glycyrrhiza spp.) is a versatile industrial and fodder crop with important medicinal, economic, and forage values. However, little is known about their salt tolerance mechanisms. Here, to investigate the osmotic adjustment mechanism of Glycyrrhiza inflata, a licorice species with excellent adaptation to the severe saline habitat, we compared the growth, the contributions of major osmolytes to osmotic potential, and the expression of genes associated with the accumulation of major osmolytes under salt stress between G. inflata and Glycyrrhiza uralensis, another licorice species mainly grown in mild salinized regions. The results showed that G. inflata displayed stronger salt tolerance than G. uralensis. Compared with G. uralensis, G. inflata accumulated higher contents of proline, betaine, and soluble sugars, as well as Na + and NO 3 − in roots, and meanwhile, accumulated higher concentrations of K + and Cl − in leaves for osmotic adjustment under salt stress. Analysis combining transcriptome and quantitative reverse transcription polymerase chain reaction indicated that compared with that in G. uralensis, the expression of key genes responsible for the biosynthesis of above organic osmolytes, the uptake of NO 3 − , and the xylem unloading and vacuolar compartmentation of Na + and NO 3 − were upregulated in roots of G. inflata under salt stress; meanwhile, higher expression levels of key genes function in xylem loading of K + and Cl − in roots and vacuolar K + and Cl − compartmentation in leaves were observed in G. inflata under salt stress. The identified key genes associated with the strong osmotic adjustment capacity of G. inflata could facilitate the genetic improvement of stress tolerance in crops. Abbreviations: AKT, inwardly rectifying K + channel; AMS, amylase; BADH, betaine aldehyde dehydrogenase; CCX, cation/Ca 2+ exchanger; CHX, cation/H + exchanger; CLC, Cl − channel; CMO, choline monooxygenase; GDH, glutamate dehydrogenase; GOGAT, glutamate synthase; HKT, high-affinity K + transporter; KT/HAK/KUP, K + transporter; MD, mannitol dehydrogenase; NHX, tonoplast Na + /H + antiporter; NRT, NO 3 − transporter; P5CS, Δ1 -pyrroline-5-carboxylate synthetase; PEAMT, phosphoethanolamine N-methyltransferase; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SKOR, stelar K + outwardly rectifying channel; SLAH, slow type anion channel; SPS, sucrose-phosphate synthase; SS, starch synthase.

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Nitrate-dependent control of shoot K homeostasis by NPF7.3/NRT1.5 and SKOR in Arabidopsis

Cited by 64 publications

References 69 publications

The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation

The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Comparative physiological, transcriptome, and qRT‐PCR analysis provide insights into osmotic adjustment in the licorice species Glycyrrhiza inflata under salt stress

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