Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiae

Petrezsélyová, Silvia; Ramos, José; Sychrová, Hana

doi:10.1007/s12223-011-0009-1

Cited by 29 publications

(23 citation statements)

References 20 publications

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“…This difference may result from the re‐uptake of lost K + by the wild‐type cells. Detailed analysis revealed that deletion of both TRK1 and TRK2 contributed to the hyperpolarization (both single mutants being more hyperpolarized than BY4741 but less hyperpolarized than the double mutant; Petrezselyova et al ., 2010a), suggesting that both systems are active and contribute to re‐uptake of lost potassium during starvation.…”

Section: Resultsmentioning

confidence: 99%

“…Deletion of TRK1 and TRK2 genes in strains with different genetic backgrounds and under various experimental conditions (Ko et al ., 1990; Ramos et al ., 1994; Madrid et al ., 1998; Bertl et al ., 2003) always results in inhibited growth at low K + concentrations, certain hyperpolarization of the plasma membrane and the observation of residual ectopic potassium transport. Whereas the phenotypes listed above are mainly connected to the deletion of TRK1 , the effect of TRK2 absence is much smaller, being almost negligible in some experimental conditions (Madrid et al ., 1998), or observable only in specific mutants (Michel et al ., 2006) and in certain reference strains (Bertl et al ., 2003; Petrezselyova et al ., 2010a).…”

Section: Introductionmentioning

confidence: 99%

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Lack of main K+ uptake systems in Saccharomyces cerevisiae cells affects yeast performance in both potassium-sufficient and potassium-limiting conditions

Navarrete

Petrezsélyová

Barreto

et al. 2010

FEMS Yeast Research

113

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A new YNB medium containing very low concentrations of alkali metal cations has been developed to carry out experiments to study potassium homoeostasis. Physiological characterization of Saccharomyces cerevisiae BY4741 strain and the corresponding mutant lacking the main potassium uptake systems (trk1 trk2) under potassium nonlimiting and limiting concentrations was performed, and novel important differences between both strains were found. At nonlimiting concentrations of KCl, the two strains had a comparable cell size and potassium content. Nevertheless, mutants were hyperpolarized, had lower pH and extruded fewer protons compared with the BY4741 strain. Upon transfer to K(+)-limiting conditions, cells of both strains became hyperpolarized and their cell volume and K(+) content diminished; however, the decrease was more relevant in BY4741. In low potassium, trk1 trk2 cells were not able to accomplish the cell cycle to the same extent as in BY4741. Moreover, K(+) limitation triggered a high-affinity K(+)/Rb(+) uptake process only in BY4741, with the highest affinity being reached as soon as 30 min after transfer to potassium-limiting conditions. By establishing basic cellular parameters under standard growth conditions, this work aims to establish a basis for the investigation of potassium homoeostasis at the system level.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lack of main K+ uptake systems in Saccharomyces cerevisiae cells affects yeast performance in both potassium-sufficient and potassium-limiting conditions

Navarrete

Petrezsélyová

Barreto

et al. 2010

FEMS Yeast Research

113

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“…The activity of Trk1 has been described to be important for K + and pH homeostasis (Madrid et al, 1998;Yenush et al, 2002), turgor (Merchan et al, 2004) and plasma membrane potential (Δw) (Madrid et al, 1998;Mulet et al, 1999). Although the potassium uptake via Trk2 is much lower than via Trk1 in exponentially growing cells (Ramos et al, 1994), a recent study showed that Trk2 activity contributes significantly to the maintenance of membrane potential in growing cells (Petrezselyova et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Borovikova

Herynkova

Rapoport

et al. 2013

FEMS Microbiol Lett

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Yeasts grow at very different potassium concentrations, adapting their intracellular cation levels to changes in the external environment. Potassium homeostasis is maintained with the help of several transporters mediating the uptake and efflux of potassium with various affinities and mechanisms. In the model yeast Saccharomyces cerevisiae, two uptake systems, Trk1 and Trk2, are responsible for the accumulation of a relatively high intracellular potassium content (200-300 mM) and the efflux of surplus potassium is mediated by the Tok1 channel and active exporters Ena ATPase and Nha1 cation/proton antiporter. Using a series of deletion mutants, we studied the role of individual potassium transporters in yeast cell resistance to dehydration. The Trk2 transporter (whose role in S. cerevisiae physiology was not clear) is important for cell viability in the stationary phase of growth and, moreover, it plays a crucial role in the yeast survival of dehydration/rehydration treatments. Mutants lacking the TRK2 gene accumulated significantly lower amounts of potassium ions in the stationary culture growth phase, and these lower amounts correlated with decreased resistance to dehydration/rehydration stress. Our results showed Trk2 to be the major potassium uptake system in stationary cells, and potassium content to be a crucial parameter for desiccation survival.

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“…The CBS573 (ATCC6258) C. krusei strain was used to amplify fragments of genomic DNA. A derivative of the S. cerevisiae BY4741 strain (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 EUROSCARF), the BYT12 strain lacking its own potassium uptake systems (trk1Δ trk2Δ; Navarrete et al, 2010;Petrezselyova, Ramos, & Sychrova, 2011), was used to express cloned C. krusei DNA fragments.…”

Section: Strains Media and Growth Conditionsmentioning

confidence: 99%

Potassium uptake systems of Candida krusei

Elicharová

Herynkova

Zimmermannová

et al. 2019

Yeast

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Candida krusei is a pathogenic yeast species that is phylogenetically outside both of the well‐studied yeast groups, whole genome duplication and CUG. Like all other yeast species, it needs to accumulate high amounts of potassium cations, which are needed for proliferation and many other cell functions. A search in the sequenced genomes of nine C. krusei strains revealed the existence of two highly conserved genes encoding putative potassium uptake systems. Both of them belong to the TRK family, whose members have been found in all the sequenced genomes of species from the Saccharomycetales subclade. Analysis and comparison of the two C. krusei Trk sequences revealed all the typical features of yeast Trk proteins but also an unusual extension of the CkTrk2 hydrophilic N‐terminus. The expression of both putative CkTRK genes in Saccharomyces cerevisiae lacking its own potassium importers showed that only CkTrk1 is able to complement the absence of S. cerevisiae's own transporters and provide cells with a sufficient amount of potassium. Interestingly, a portion of the CkTrk1 molecules were localized to the vacuolar membrane. The presence of CkTrk2 had no evident phenotype, due to the fact that this protein was not correctly targeted to the S. cerevisiae plasma membrane. Thus, CkTrk2 is the first studied yeast Trk protein to date that was not properly recognized and targeted to the plasma membrane upon heterologous expression in S. cerevisiae.

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Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiae

Cited by 29 publications

References 20 publications

Lack of main K+ uptake systems in Saccharomyces cerevisiae cells affects yeast performance in both potassium-sufficient and potassium-limiting conditions

Lack of main K+ uptake systems in Saccharomyces cerevisiae cells affects yeast performance in both potassium-sufficient and potassium-limiting conditions

Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis

Potassium uptake systems of Candida krusei

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