S1P–S1PR2 Axis Mediates Homing of Muse Cells Into Damaged Heart for Long-Lasting Tissue Repair and Functional Recovery After Acute Myocardial Infarction

Yamada, Yoshihisa; Wakao, Shohei; Kushida, Yoshihiro; Minatoguchi, Shingo; Mikami, Atsushi; Higashi, Kenshi; Baba, Shinya; Shigemoto, Taeko; Kuroda, Yasumasa; Kanamori, Hiromitsu; Amin, Mohamad; Kawasaki, Masanori; Nishigaki, Kazuhiko; Taoka, M; Isobe, Toshiaki; Muramatsu, Chisako; Dezawa, Mari; Minatoguchi, Shinya

doi:10.1161/circresaha.117.311648

Cited by 118 publications

(217 citation statements)

References 39 publications

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“…Because bone marrow‐derived mesenchymal stromal cells (MSCs) were confirmed to possess significant self‐renewability, low immunogenicity, and ability to differentiate into a variety of specialized cells (Oreffo, Cooper, Mason, & Clements, ; Pittenger et al, ), it has been wildly applied to cell‐based strategies for tissue engineering and regenerative medicine such as treatment of ischemia‐reperfusion injury and heart diseases (Lo et al, ; Yamada et al, ). Studies have reported that transplantation of MSCs could lead to improved neovascularization of ischemic myocardium, inhibition of myocardial fibrosis, and restoration of myocardial contractile function (Epstein, Luger, & Lipinski, ; Yan et al, ).…”

Section: Introductionmentioning

confidence: 99%

The protective effects of HMGA2 in the senescence process of bone marrow‐derived mesenchymal stromal cells

Yang

Wang

et al. 2020

J Tissue Eng Regen Med

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Bone marrow-derived mesenchymal stromal cells (MSCs) have been wildly applied to cell-based strategies for tissue engineering and regenerative medicine; however, they have to undergo the senescence process and thus appeared to be less therapeutic effective. HMGA2, a protein belonged to high mobility group A (HMGA) family, exhibits an inverse expression level related to embryonic development and acts as a developmental regulator in stem cell self-renewal progression. Therefore, we performed senescence-associated β-galactosidase (SA-β-gal) staining, transwell assay, to examine the changes of MSCs in different stages and then over-expressed HMGA2 in MSCs by lentivirus transfection. We found the percentage of SA-β-gal staining positive cells in MSCs from 24-month-old Sprague-Dawley (SD) rats (O-MSCs) was significantly higher compared with MSCs from 2-week-old SD rats (Y-MSCs), and the expression levels of P21 and P53, two senescence-related molecules, were also significantly up-regulated in O-MSCs than in Y-MSCs. In contrast, the HMGA2 expression level in O-MSCs was dramatically down-regulated in contrast to Y-MSCs. In additional, the migration ability in O-MSCs was significantly attenuated than in Y-MSCs. After successfully over-expressed HMGA2 in O-MSCs, the percentage of SAβ-gal staining positive cells and the expression levels of P21 and P53 were reduced, and the migration ability was improved compared with O-MSCs without treatment. Further, mRNA sequencing analysis revealed that overexpression of HMGA2 changed the expression of genes related to cell proliferation and senescence, such as Lyz2, Pf4, Rgs2, and Mstn. Knockdown of Rgs2 in HMGA2 overexpression O-MSCs could antagonize the protective effect of HMGA2 in the senescence process of O-MSCs.

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Section: Introductionmentioning

confidence: 99%

The protective effects of HMGA2 in the senescence process of bone marrow‐derived mesenchymal stromal cells

Yang

Wang

et al. 2020

J Tissue Eng Regen Med

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“…Furthermore, if the S1PR2 gene was knocked out in Muse cells, they would lose the homing feature. These results suggest that the S1P-S1PR2 axis is the mechanism for Muse cell homing to lesion areas [22].…”

Section: Characteristics Of Muse Cellsmentioning

confidence: 72%

“…Gene analysis showed that CDH1 was overexpressed and alpha-6 integrin (ITGA6) and Lin28 were down-regulated in Muse cells [4,6,20,21]. Animal studies in the testis of severe combined immunodeficient mice (SCID) and myocardial infarction sites of rabbits demonstrated that injected Muse cells did not form tumors 6 months post-injection [1,4,22]. Moreover, Muse cells are a subpopulation of MSCs, which have been safely transplanted in basic and clinical studies [23,24].…”

Section: Characteristics Of Muse Cellsmentioning

confidence: 99%

“…For strokes, spinal cord injuries and other acute central nervous system injuries, cell therapies are best if carried out before formation of glial scars. Muse cells make this timeline possible [22].…”

Section: Characteristics Of Muse Cellsmentioning

confidence: 99%

“…However, these cell groups face barriers in cell source, ethics, tumorigenicity or limited functional recovery [46]. Studies have shown that Muse cells could migrate to and home into damaged areas by intravenous (IV) injection [22,31,[47][48][49][50][51][52], which makes them practical for regenerative therapy. More basic research and high-quality studies, such as multicenter, random, double blind, and placebo-control clinical trials need to be carried out.…”

Section: Summary: Muse Cells May Revolutionize Regenerative Medicinementioning

confidence: 99%

See 2 more Smart Citations

Muse cells and Neurorestoratology

Leng

Kethidi

Chang

et al. 2019

Journal of Neurorestoratology

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Multilineage-differentiating stress-enduring (Muse) cells were discovered in 2010 as a subpopulation of mesenchymal stroma cells (MSCs). Muse cells can self-renew and tolerate severe culturing conditions. These cells can differentiate into three lineage cells spontaneously or in induced medium but do not form teratoma in vitro or in vivo. Central nervous system (CNS) diseases, such as intracerebral hemorrhage (ICH), cerebral infarction, and spinal cord injury are normally disastrous. Despite numerous therapy strategies, CNS diseases are difficult to recover. As a novel kind of pluripotent stem cells, Muse cells have shown great regeneration capacity in many animal models, including acute myocardial infarction, hepatectomy, and acute cerebral ischemia (ACI). After injection into injury sites, Muse cells survived, migrated, and differentiated into functional neurons with synaptic junctions to local neurons and contributed to recovery of function. Furthermore, Muse cell differentiation did not need to be induced pre-transplantation and no tumors were observed post- transplantation. The Muse cell population is promising and may lead to a revolution in regenerative medicine. This review focuses on recent advances regarding the Muse cells therapies in Neurorestoratology and discusses future perspectives in this field.

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A Museum of Stem Cells Points to Muse Cells as Robust Transplantable Cells for Stroke: Review

Park

Farooq

Cho

et al. 2020

Advances in Experimental Medicine and Biology

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S1P–S1PR2 Axis Mediates Homing of Muse Cells Into Damaged Heart for Long-Lasting Tissue Repair and Functional Recovery After Acute Myocardial Infarction

Abstract: Muse cells may provide reparative effects and robust functional recovery and may, thus, provide a novel strategy for the treatment of acute myocardial infarction.

Cited by 118 publications

References 39 publications

The protective effects of HMGA2 in the senescence process of bone marrow‐derived mesenchymal stromal cells

The protective effects of HMGA2 in the senescence process of bone marrow‐derived mesenchymal stromal cells

Muse cells and Neurorestoratology

A Museum of Stem Cells Points to Muse Cells as Robust Transplantable Cells for Stroke: Review

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