S-nitrosoglutathione-containing hydrogel increases dermal blood flow in streptozotocin-induced diabetic rats

Seabra, Amedea B.; Pankotai, Eszter; Fehér, M; Somlai, Á; Kiss, Levente; Biró, László Péter; Szabó, Csaba; Kollai, Márk; Oliveira, Marcelo Ganzarolli de; Lacza, Zsombor

doi:10.1111/j.1365-2133.2006.07718.x

Cited by 64 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In mammals, NO is considered to circulate as S-nitrosothiols, mainly S-nitrosoglutathione (GSNO), S-nitrosoalbumin and pos sibly S-nitrosohemoglobin (3,4) . Synthetic GSNO and S-nitroso-Nace tylcysteine (SNAC) were already used as exogenous NO donors in different experimental settings (5,6) . In the eye, NO has been shown to be a key regulator of vascular tone in ophthalmic arteries (7,8) and animal and human studies have demonstrated reduction in the choroidal blood flow with systemic infusion of NO inhibitors (9)(10)(11) .…”

Section: Introductionmentioning

confidence: 99%

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

Cariello¹,

Souza²,

Lowen³

et al. 2013

Arq. Bras. Oftalmol.

View full text Add to dashboard Cite

Ethic committee from UNIFESP: number 1573/08. ABSTRACTPurpose: To evaluate the ocular surface toxicity of two nitric oxide donors in ex vivo and in vivo animal models: S-nitrosoglutathione (GSNO) and S-nitroso-Nace tylcysteine (SNAC) in a hydroxypropyl methylcellulose (HPMC) matrix at final concentrations 1.0 and 10.0 mM. Methods: Ex vivo GSNO and SNAC toxicities were clinically and histologically ana lyzed using freshly excised pig eyeballs. In vivo experiments were performed with 20 albino rabbits which were randomized into 4 groups (5 animals each): Groups 1 and 2 received instillations of 150 µL of aqueous HPMC solution containing GSNO 1.0 and 10.0 mM, respectively, in one of the eyes; Groups 3 and 4 received instillations of 150 µL of aqueous HPMC solution-containing SNAC 1.0 and 10.0 mM, respectively, in one of the eyes. The contralateral eyes in each group received aqueous HPMC as a control. All animals underwent clinical evaluation on a slit lamp and the eyes were scored according to a modified Draize eye test and were histologically analyzed. Results: Pig eyeballs showed no signs of perforation, erosion, corneal opacity or other gross damage. These findings were confirmed by histological analysis. The re was no difference between control and treated rabbit eyes according to the Draize eye test score in all groups (p>0.05). All formulations showed a mean score under 1 and were classified as "non-irritating". There was no evidence of tissue toxicity in the histological analysis in all animals. Conclusion: Aqueous HPMC solutions containing GSNO and SNAC at concentrations up to 10.0 mM do not induce ocular irritation.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

Cariello¹,

Souza²,

Lowen³

et al. 2013

Arq. Bras. Oftalmol.

View full text Add to dashboard Cite

show abstract

“…At high blood flow levels there is a reduction in dermal nitrite, which is ascribed to increased blood scavenging. To further investigate the vasodilatory capacity and the possible side-effects of topical application, GSNO incorporated PluronicsF127 hydrogel was applied on the foot sole skin of healthy and streptozotocin-induced diabetic rats [183]. The hydrogel alone did not induce any changes in microvascular flow, while GSNO-containing hydrogel caused a twofold increase in perfusion.…”

Section: Nitric Oxide In Wound Healingmentioning

confidence: 99%

Nitric oxide based strategies for applications of biomedical devices

Yang

et al. 2015

Biosurface and Biotribology

View full text Add to dashboard Cite

Since the first report describing nitric oxide (NO) in the mid-1980s, research efforts have been devoted to elucidating the pathway of NO generation and the mechanism of NO function in vivo. The worldwide expansion of NO investigations have helped advance progress in biomedical device applications of NO-based strategies for cardiovascular devices, wound healing and antimicrobial agents, including recent in vivo investigations. Here we describe the general concepts of NO discovery, mechanisms of NO synthesis in vivo and NO producing materials. Both design strategies and results are discussed and compared in release kinetics, NO dose and biological effects, with the aim of providing foundations for the development of new NO-based therapeutics. & 2015 Southwest Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

show abstract

“…However, NO has a short half-life in vivo, of the order of seconds. In an attempt to develop NO-donors which could provide controlled and prolonged release of NO, several types of NO-donors have been designed and evaluated both in vivo and in vitro [34][35][36][37][38][39][40]. Recently, NO-donors-used as restenosis inhibitors-have also been incorporated into stents [37,[39][40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

The formation of an anti-restenotic/anti-thrombotic surface by immobilization of nitric oxide synthase on a metallic carrier

et al. 2014

View full text Add to dashboard Cite

S-nitrosoglutathione-containing hydrogel increases dermal blood flow in streptozotocin-induced diabetic rats

Abstract: Dermal application of GSNO may be an effective treatment for promoting the local vasodilation in both healthy and diabetic states, without inducing protein nitration or alterations in blood pressure or heart rate.

Cited by 64 publications

References 33 publications

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

Nitric oxide based strategies for applications of biomedical devices

The formation of an anti-restenotic/anti-thrombotic surface by immobilization of nitric oxide synthase on a metallic carrier

Contact Info

Product

Resources

About