Rutin Inhibits Oleic Acid Induced Lipid Accumulation via Reducing Lipogenesis and Oxidative Stress in Hepatocarcinoma Cells

Abstract

Excessive lipid accumulation within liver has been proposed to cause obesity, hyperlipidemia, diabetes, and fatty liver disease. Rutin, a common dietary flavonoid that is consumed in fruits, vegetables, and plant-derived beverages, has various biological functions, including antioxidant, anti-inflammatory, and anticancer effects. However, a hypolipidemic effect of rutin on fatty liver disease has not been reported. In this study, we examined the effect of rutin on reducing lipid accumulation in hepatic cells. Hepatocytes were treated with oleic acid (OA) containing with or without rutin to observe the lipid accumulation by Nile red stain. The result showed rutin suppressed OA-induced lipid accumulation and increased adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) activity in hepatocytes. The expression of critical molecule involved in lipid synthesis, sterol regulatory element binding proteins-1 (SREBP-1), was attenuated in rutin-treated cells. Moreover, long-term incubation of rutin inhibited the transcriptions of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (HMGCR), glycerol-3-phosphate acyltransferase (GPAT), fatty acid synthase (FAS), and acetyl-coenzyme carboxylase (ACC). Besides, we also found out the antioxidative effect of rutin by increasing the expression of peroxisome proliferator-activated receptor (PPAR)-α and antioxidative enzymes. Taken together, our findings suggest rutin could attenuate lipid accumulation by decreasing lipogenesis and oxidative stress in hepatocyte.

Effect of Centaurium erythraea Rafn, Artemisia herba-alba Asso and Trigonella foenum-graecum L. on liver fat accumulation in C57BL/6J mice with high-fat diet-induced type 2 diabetes

“…The benefits of polyphenols in suppressing TG accumulation in hepatocytes have been also reported in previous studies. Wu et al (2011) proved that rutin can significantly reduce lipid accumulation and had a good antioxidant capacity in an oleic acid-induced in vitro model of fatty liver by decreasing lipogenesis and oxidative stress in hepatocytes. The benefit of phenolic compounds on preventing steatosis has also been observed.…”

Amelioration of palmitate-induced insulin resistance in C2C12 muscle cells by rooibos (Aspalathus linearis)

“…In C2C12 muscle cells, Eid et al (2010) reported that quercetin increased AMPK phosphorylation but not AKT. Rutin has been shown to increase phosphorylation of AMPK and reduce SREBP-1 expression thereby inhibiting hepatic lipogenesis (Wu et al 2011). In another study conducted by Lee et al (2012) rutin and the combination of rutin and quercetin improved glucose uptake and activated AKT and AMPK in hepatocytes.…”

Sechium edule Shoot Extracts and Active Components Improve Obesity and a Fatty Liver That Involved Reducing Hepatic Lipogenesis and Adipogenesis in High-Fat-Diet-Fed Rats

“…20 Sechium edule shoots (SWE) contain a lot of nutritional components, including flavonoids, 21 which are known as powerful polyphenol antioxidants. 17 They are useful as a complementary treatment for arteriosclerosis and hypertension and as a diuretic and anti-inflammatory remedy.…”