Rubinisphaera italica sp. nov. isolated from a hydrothermal area in the Tyrrhenian Sea close to the volcanic island Panarea

Abstract

Planctomycetes is a fascinating phylum of mostly aquatic bacteria, not only due to the environmental importance in global carbon and nitrogen cycles, but also because of a unique cell biology. Their lifestyle and metabolic capabilities are not well explored, which motivated us to study the role of Planctomycetes in biofilms on marine biotic surfaces. Here, we describe the novel strain Pan54 T which was isolated from algae in a hydrothermal area close to the volcanic island Panarea in the Tyrrhenian Sea, north of Sicily in Italy. The strain grew best at pH 9.0 and 26°C and showed typical characteristics of planctomycetal bacteria, e.g. division by polar budding, formation of aggregates and presence of stalks and crateriform structures. Phylogenetically, the strain belongs to the genus Rubinisphaera. Our analysis suggests that Pan54 T represents a novel species of this genus, for which we propose the name Rubinisphaera italica sp. nov. We suggest Pan54 T (= DSM 29369 = LMG 29789) as the type strain of the novel species.

Aureliella helgolandensis gen. nov., sp. nov., a novel Planctomycete isolated from a jellyfish at the shore of the island Helgoland

“…For the isolation and cultivation of strain Q31a T , M1H NAG ASW medium was used (Kallscheuer et al 2019a). Strain Q31a T was isolated from a dead common jellyfish (Aurelia aurita) found at the shore of Helgoland Island (exact location 54.188 N 7.875 E) on the 5th of June 2013.…”

“…16S rRNA gene-based phylogeny was computed for Q31a T , the type strains of all described planctomycetal species (assessed in January 2020) and all isolates recently described (Boersma et al 2019;Kallscheuer et al 2019aKallscheuer et al , b, d, 2020Kohn et al 2019;Peeters et al 2020;Rensink et al 2020). The 16S rRNA gene sequences were aligned with SINA (Pruesse et al 2012) and the phylogenetic inference was calculated with RAxML (Stamatakis 2014) with a maximum likelihood approach with 1000 bootstraps, nucleotide substitution model GTR, gamma distributed rate variation and estimation of proportion of invariable sites (GTRGAMMAI option).…”

Description of Polystyrenella longa gen. nov., sp. nov., isolated from polystyrene particles incubated in the Baltic Sea

“…16S rRNA gene-based phylogeny was computed for strain Pla110 T , the type strains of all described planctomycetal species (assessed in January 2020), all isolates recently described (Boersma et al 2019;Kallscheuer et al 2019aKallscheuer et al , b, c, d, 2020Kohn et al 2019;Peeters et al 2019;Rensink et al 2020;) and with an outgroup of strains from outside the Planctomycetes, but part of the PVC superphylum. The alignment of 16S rRNA genes was made with SINA (Pruesse et al 2012).…”

Maioricimonas rarisocia gen. nov., sp. nov., a novel planctomycete isolated from marine sediments close to Mallorca Island

“…For strain isolation and cultivation M1H NAG ASW medium was used. Medium preparation was previously described (Kallscheuer et al 2019a). Cultures were incubated in baffled flasks at 28°C with constant agitation at 110 rpm.…”