Maioricimonas rarisocia gen. nov., sp. nov., a novel planctomycete isolated from marine sediments close to Mallorca Island

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Planctomycetes is a phylum of environmentally important bacteria, which also receive significant attention due to their fascinating cell biology. Access to axenic Planctomycete cultures is crucial to study cell biological features within this phylum in further detail. In this study, we characterise three novel strains, Mal52 T , Pan258 and CA54 T , which were isolated close to the coasts of the islands Mallorca (Spain) and Panarea (Italy), and from Monterey Bay, CA, USA. The three isolates show optimal growth at temperatures between 22 and 24°C and at pH 7.5, divide by polar budding, lack pigmentation and form strong aggregates in liquid culture. Analysis of five phylogenetic markers suggests that the strains constitute two novel species within a novel genus in the family Planctomycetaceae. The strains Mal52 T (DSM 101177 T = VKM B-3432 T) and Pan258 were assigned to the species Symmachiella dynata gen nov., sp. nov., while strain CA54 T (DSM 104301 T = VKM B-3450 T) forms a separate species of the same genus, for which we propose the name Symmachiella macrocystis sp. nov.

Section: Phylogenetic Inferencementioning

confidence: 90%

Three Planctomycetes isolated from biotic surfaces in the Mediterranean Sea and the Pacific Ocean constitute the novel species Symmachiella dynata gen. nov., sp. nov. and Symmachiella macrocystis sp. nov.

Salbreiter

Waqqas

Jogler

et al. 2020

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“…The 16S rRNA gene sequence-based phylogeny was computed for strain Pan265 T , the type strains of all described planctomycetal species (assessed in May 2021) including all isolates published and described in the last two years (Dedysh et al 2019a , 2019b ; Kallscheuer et al 2020a , 2020b , 2020e ; Kohn et al 2020 , 2019 ; Peeters et al 2020 ; Rivas-Marin et al 2020a , 2020b ; Vitorino et al 2020 ; Waqqas et al 2020 ). The 16S rRNA gene sequences were aligned with SINA (Pruesse et al 2012 ) and the phylogenetic inference was calculated with RAxML (Stamatakis 2014 ) with a maximum likelihood approach with 1,000 bootstraps, nucleotide substitution model GTR, gamma distributed rate variation and estimation of proportion of invariable sites (GTRGAMMAI option).…”

Section: Methodsmentioning

confidence: 99%

Mucisphaera calidilacus gen. nov., sp. nov., a novel planctomycete of the class Phycisphaerae isolated in the shallow sea hydrothermal system of the Lipari Islands

Kallscheuer

Jogler

Peeters

et al. 2022

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For extending the current collection of axenic cultures of planctomycetes, we describe in this study the isolation and characterisation of strain Pan265T obtained from a red biofilm in the hydrothermal vent system close to the Lipari Islands in the Tyrrhenian Sea, north of Sicily, Italy. The strain forms light pink colonies on solid medium and grows as a viscous colloid in liquid culture, likely as the result of formation of a dense extracellular matrix observed during electron microscopy. Cells of the novel isolate are spherical, motile and divide by binary fission. Strain Pan265T is mesophilic (temperature optimum 30–33 °C), neutrophilic (pH optimum 7.0–8.0), aerobic and heterotrophic. The strain has a genome size of 3.49 Mb and a DNA G + C content of 63.9%. Phylogenetically, the strain belongs to the family Phycisphaeraceae, order Phycisphaerales, class Phycisphaerae. Our polyphasic analysis supports the delineation of strain Pan265T from the known genera in this family. Therefore, we conclude to assign strain Pan265T to a novel species within a novel genus, for which we propose the name Mucisphaera calidilacus gen. nov., sp. nov. The novel species is the type species of the novel genus and is represented by strain Pan265T (= DSM 100697T = CECT 30425T) as type strain.

“…1), strain Pan44 T was observed to cluster within the family Planctomycetaceae, which is currently the sole family within the order Planctomycetales. All investigated phylogenetic markers (16S rRNA gene identity, rpoB identity, AAI, ANI and POCP) suggest Maioricimonas rarisocia Mal4 T and Planctomicrobium piriforme P3 T to be the current closest neighbours (Kulichevskaya et al 2015;Rivas-Marin et al 2020b). ANI values of 69.9% and 69.4%, respectively, indicate that strain Pan44 T is not a member of the species M. rarisocia or P. piriforme.…”

Section: Phylogenetic Inferencementioning

confidence: 96%

Caulifigura coniformis gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from a red biofilm sampled in a hydrothermal area

Kallscheuer

Wiegand

Boedeker

et al. 2020

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Pan44 T , a novel strain belonging to the phylum Planctomycetes, was isolated from a red biofilm in a hydrothermal area close to the island Panarea in the Tyrrhenian Sea north of Sicily, Italy. The strain forms white colonies on solid medium and displays the following characteristics: cell division by budding, formation of rosettes, presence of matrix or fimbriae and long stalks. The cell surface has an interesting and characteristic texture made up of triangles and rectangles, which leads to a pine conelike morphology of the strain. Strain Pan44 T is mesophilic (temperature optimum 26°C), slightly alkaliphilic (pH optimum 8.0), aerobic and heterotrophic. The strain has a genome size of 6.76 Mb with a G ? C content of 63.2%. Phylogenetically, the strain is a member of the family Planctomycetaceae, order Planctomycetales, class Planctomycetia. Our analysis supports delineation of strain Pan44 T from all known genera in this family, hence, we propose to assign it to a novel species within a novel genus, for which we propose the name Caulifigura coniformis gen. nov., sp. nov., represented by Pan44 T (DSM 29405 T = LMG 29788 T) as the type strain.