Aureliella helgolandensis gen. nov., sp. nov., a novel Planctomycete isolated from a jellyfish at the shore of the island Helgoland

Kallscheuer, Nicolai; Wiegand, Sandra; Boedeker, Christian; Peeters, Stijn H.; Jogler, Mareike; Rast, Patrick; Heuer, Anja; Jetten, Mike S. M.; Rohde, Manfred; Jogler, Christian

doi:10.1007/s10482-020-01403-8

Cited by 20 publications

(6 citation statements)

References 52 publications

(55 reference statements)

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“…No planctomycetes were obtained from the anemone Actinia equina in either sampling period. Planctomycetes have already been associated to different invertebrates, such as crustaceans, jellyfish, and sponges [5,8,9,11,[60][61][62]. Furthermore, in a recent study that characterized the gut microbiome of the mussel Mytilus galloprovincialis, [63] members of Fuerstiella sp., Phycisphaera sp., Blastopirella sp., and diverse unclassified planctomycetes were detected by metagenomic analysis.…”

Section: Isolation Of Planctomycetes From the Sea Water Column And Marine Invertebrates And Sedimentsmentioning

confidence: 99%

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

et al. 2021

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Bacteria from the distinctive Planctomycetes phylum are well spread around the globe; they are capable of colonizing many habitats, including marine, freshwater, terrestrial, and even extreme habitats such as hydrothermal vents and hot springs. They can also be found living in association with other organisms, such as macroalgae, plants, and invertebrates. While ubiquitous, only a small fraction of the known diversity includes axenic cultures. In this study, we aimed to apply conventional techniques to isolate, in diverse culture media, planctomycetes from two beaches of the Portuguese north-coast by using sediments, red, green, and brown macroalgae, the shell of the mussel Mytilus edulis, an anemone belonging to the species Actinia equina, and seawater as sources. With this approach, thirty-seven isolates closely related to seven species from the families Planctomycetaceae and Pirellulaceae (class Planctomycetia) were brought into pure culture. Moreover, we applied an iChip inspired in-situ culturing technique to successfully retrieve planctomycetes from marine sediments, which resulted in the isolation of three additional strains, two affiliated to the species Novipirellula caenicola and one to a putative novel Rubinisphaera. This work enlarges the number of isolated planctomycetal strains and shows the adequacy of a novel methodology for planctomycetes isolation.

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Section: Isolation Of Planctomycetes From the Sea Water Column And Marine Invertebrates And Sedimentsmentioning

confidence: 99%

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

et al. 2021

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“…The first report of the isolation of a planctomycete in axenic cultures is due to the work of James T. Staley (Staley, 1973). During the last decade, several members of Planctomycetes were isolated in pure culture and described as representing new genera, namely Schlesneria (Kulichevskaya et al, 2007b), Singulisphaera (Kulichevskaya et al, 2008;Kulichevskaya et al, 2012a), Zavarzinella (Kulichevskaya et al, 2009), Aquisphaera (Bondoso et al, 2011), Telmatocola (Kulichevskaya et al, 2012b), Paludisphaera (Kulichevskaya et al, 2016;Kaushik et al, 2020), Roseimaritima (Bondoso et al, 2015;Kumar et al, 2020b), Rubripirellula (Bondoso et al, 2015;Kallscheuer et al, 2019b), Fimbriiglobus (Kulichevskaya et al, 2017a), Tundrisphaera (Kulichevskaya et al, 2017b), Mariniblastus (Lage et al, 2017), Caulifigura (Kallscheuer et al, 2020a), Tuwongella (Seeger et al, 2017), Crateriforma (Peeters et al, 2020c), Fuerstia (Kohn et al, 2016;Kohn et al, 2019), Novipirellula (Kallscheuer et al, 2019c), Tautonia (Kovaleva et al, 2019;Jogler et al, 2020) Limnoglobus (Kulichevskaya et al, 2020b), Thalassoglobus (Kohn et al, 2020a;Rivas-Marin et al, 2020c), Symmachiella (Salbreiter et al, 2020), Gimesia (Scheuner et al, 2014;Kumar et al, 2020a;Wang et al, 2020), Stieleria (Sandargo et al, 2020;Storesund et al, 2020;Surup et al, 2020), Aureliella (Kallscheuer et al, 2020b), Maioricimonas (Rivas-Marin et al, 20...…”

Section: Cultured Planctomycetesmentioning

confidence: 99%

“…They require a specific culture medium and long incubation times (Franzmann and Skerman, 1984;Lee et al, 2009;Wiegand et al, 2020). Accordingly, most knowledge regarding the environmental and host-associated microbiota niches of Planctomycetes organisms is derived from DNA-based studies including PCR-sequencing-based analyses and metagenomics studies (Cayrou et al, 2013;Bondoso et al, 2014;Drancourt et al, 2014;Delmont et al, 2018;Dedysh et al, 2020a), although there have been considerable advances to date, that have made it possible to isolate many species of this phylum from host-associated Planctomycetes from environmental habitats (Dedysh et al, 2020b;Jogler et al, 2020;Kallscheuer et al, 2020b;Kaushik et al, 2020;Kohn et al, 2020b;Kulichevskaya et al, 2020a;Kulichevskaya et al, 2020b;Rivas-Marin et al, 2020b;Rivas-Marin et al, 2020c;Salbreiter et al, 2020;Sandargo et al, 2020;Schubert et al, 2020;Wang et al, 2020;Wiegand et al, 2020). Hence, enormous work remains to be done to improve their culture and their isolation, in view of the enormity of 16S rRNA genes sequenced deposited in GenBank but have never yet been isolated in pure culture (uncultured Planctomycetes) up to the present day (see review Wiegand et al, 2018 for more details) because, the true knowledge of bacterial taxonomy, physiology, and pathogenicity has long time based only on the isolation of the strain in pure culture and presents several challenges for clinical microbiologists.…”

Section: Introductionmentioning

confidence: 99%

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Kaboré

Godreuil

Drancourt

2020

Front. Cell. Infect. Microbiol.

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Traditionally recognized as environmental bacteria, Planctomycetes have just been linked recently to human pathology as opportunistic pathogens, arousing a great interest for clinical microbiologists. However, the lack of appropriate culture media limits our future investigations as no Planctomycetes have ever been isolated from patients’ specimens despite several attempts. Several Planctomycetes have no cultivable members and are only recognized by 16S rRNA gene sequence detection and analysis. The cultured representatives are slow-growing fastidious bacteria and mostly difficult to culture on synthetic media. Accordingly, the provision of environmental and nutritional conditions like those existing in the natural habitat where yet uncultured/refractory bacteria can be detected might be an option for their potential isolation. Hence, we systematically reviewed the various natural habitats of Planctomycetes, to review their nutritional requirements, the physicochemical characteristics of their natural ecological niches, current methods of cultivation of the Planctomycetes and gaps, from a perspective of collecting data in order to optimize conditions and the protocols of cultivation of these fastidious bacteria. Planctomycetes are widespread in freshwater, seawater, and terrestrial environments, essentially associated to particles or organisms like macroalgae, marine sponges, and lichens, depending on the species and metabolizable polysaccharides by their sulfatases. Most Planctomycetes grow in nutrient-poor oligotrophic environments with pH ranging from 3.4 to 11, but a few strains can also grow in quite nutrient rich media like M600/M14. Also, a seasonality variation of abundance is observed, and bloom occurs in summer-early autumn, correlating with the strong growth of algae in the marine environments. Most Planctomycetes are mesophilic, but with a few Planctomycetes being thermophilic (50°C to 60°C). Commonly added nutrients are N-acetyl-glucosamine, yeast-extracts, peptone, and some oligo and macro-elements. A biphasic host-associated extract (macroalgae, sponge extract) conjugated with a diluted basal medium should provide favorable results for the success of isolation in pure culture.

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“…16S rRNA gene sequence-based phylogeny was computed for strains Poly24 T , CA51 T and Mal33 and the type strains of all described planctomycetal species (as of May 2020), including recently published strains (Boersma et al 2019;Dedysh et al 2020a;Dedysh et al 2020b;Kallscheuer et al 2019a;Kallscheuer et al 2020a;Kallscheuer et al 2020b;Kallscheuer et al 2019c;Kallscheuer et al 2019d;Kohn et al 2020;Kumar et al 2020;Peeters et al 2020;Rensink et al 2020). An alignment of 16S rRNA gene sequences was made with SINA (Pruesse et al 2012).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA

Waqqas

Salbreiter

Kallscheuer

et al. 2020

Antonie van Leeuwenhoek

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Planctomycetes are ubiquitous bacteria with fascinating cell biological features. Strains available as axenic cultures in most cases have been isolated from aquatic environments and serve as a basis to study planctomycetal cell biology and interactions in further detail. As a contribution to the current collection of axenic cultures, here we characterise three closely related strains, Poly24 T , CA51 T and Mal33, which were isolated from the Baltic Sea, the Pacific Ocean and the Mediterranean Sea, respectively. The strains display cell biological features typical for related Planctomycetes, such as division by polar budding, presence of crateriform structures and formation of rosettes. Optimal growth was observed at temperatures of 30-33°C and at pH 7.5, which led to maximal growth rates of 0.065-0.079 h-1 , corresponding to generation times of 9-11 h. The genomes of the novel isolates have a size of 7.3-7.5 Mb and a G ? C content of 57.7-58.2%. Phylogenetic analyses place the strains in the family Pirellulaceae and suggest that Roseimaritima ulvae and Roseimaritima sediminicola are the current closest relatives. Analysis of five different phylogenetic markers, however, supports the delineation of the strains from members of the genus Roseimaritima and other characterised genera in the family. Supported by morphological and physiological differences, we conclude that the strains belong to the novel genus Rosistilla gen. nov. and constitute two novel species, for which we propose the names Rosistilla carotiformis sp. nov. and Rosistilla oblonga sp. nov. (the type species). The two novel species are represented by the type strains Poly24 T

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Aureliella helgolandensis gen. nov., sp. nov., a novel Planctomycete isolated from a jellyfish at the shore of the island Helgoland

Cited by 20 publications

References 52 publications

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA

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