Ru(bpy)32+-doped silica nanoparticle DNA probe for the electrogenerated chemiluminescence detection of DNA hybridization

Zhu, Chen; Zhou, Jingming; Zhao, Kun; Zhu, Ning; He, Pingang; Fang, Yuzhi

doi:10.1016/j.electacta.2006.05.036

Cited by 94 publications

(59 citation statements)

References 33 publications

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“…[1e, 11,21] When the RuSi nanoparticles within LBL coatings presented herein are used as ECL tags, possible leaking of [RuA C H T U N G T R E N N U N G (bpy) 3 ] 2 + ions must be considered. The ECL behavior of the [RuA C H T U N G T R E N N U N G (bpy) 3 ] 2 + ions leaked from the particles during the study of the ECL of the RuSi nanoparticles deposited with biomolecules was inspected (see the Experimental Section).…”

Section: Resultsmentioning

confidence: 99%

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[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

Wei

Liu

Zhou

et al. 2008

Chemistry A European J

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[Ru(bpy)3]2+-doped silica (RuSi) nanoparticles were synthesized by using a water/oil microemulsion method. Stable electrochemiluminescence (ECL) was obtained when the RuSi nanoparticles were immobilized on a glassy carbon electrode by using tripropylamine (TPA) as a coreactant. Furthermore, the ECL of the RuSi nanoparticles with layer-by-layer biomolecular coatings was investigated. Squential self-assembly of the polyelectrolytes and biomolecules on the RuSi nanoparticles gave nanocomposite suspensions, the ECL of which decreased on increasing the number of bilayers. Moreover, factors that affected the assembly and ECL signals were investigated. The decrease in ECL could be assigned to steric hindrance and limited diffusion of the coreactant molecules in the silica matrix after they were attached to the biomolecules. Since surface modification of the RuSi nanoparticles can improve their biocompatibility and prevent leaking of the [Ru(bpy)3]2+ ions, the RuSi nanoparticles can be readily used as efficient and stable ECL tag materials in immunoassay and DNA detection.

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Section: Resultsmentioning

confidence: 99%

“…[12c, 20] Fang et al used RuSi nanoparticles as ECL tags to detect DNA hybridization. [21] However, RuSi nanoparticles without further surface modification suffered from leaking of the [RuA C H T U N G T R E N N U N G (bpy) 3 ] 2 + ions. Thus, it is interesting and important to develop stable ECL tags based on RuSi nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

Wei

Liu

Zhou

et al. 2008

Chemistry A European J

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“…[24] In their study, Ru(bpy) 3 2+ is enriched and restricted within the network of silica with electrostatic interactions so as to prevent its leaching, since hydroxyl groups on the surfaces of silica nanoparticles interact with the Ru(bpy) 3 2+ cation. Some ECL sensors based on RuSiNPs have been developed to determine DNA, [25] enzymes, [26] amines [27,28] and proteins. [29] Methamphetamine (METH) is a potent stimulant to the central nervous system.…”

Section: +mentioning

confidence: 99%

Electrochemiluminescence detection of methamphetamine based on a Ru(bpy)₃²⁺‐doped silica nanoparticles/Nafion composite film modified electrode

et al. 2009

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An electrochemiluminescence (ECL) approach for methamphetamine determination was developed based on a glassy carbon electrode modified with a Ru(bpy)₃ (2+)-doped silica nanoparticles/Nafion composite film. The monodispersed nanoparticles, which were about 50 nm in size, were synthesized using the water-in-oil microemulsion method. The ECL results revealed that Ru(bpy)₃ (2+) doped in silica nanoparticles retained its original photo- and electrochemical properties. The ECL intensity was found to be proportional to methamphetamine concentration over the range from 1.0 × 10(-7) to 1.0 × 10(-5) mol L(-1), and the detection limit was found to be 2.6 × 10(-8) mol L(-1). The proposed ECL approach was used to analyze the methamphetamine content in drugs.

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“…The electrochemiluminescent (ECL) generation of Ru(III) complexes is receiving a lot of interest due to the favourable characteristics of these species, such as excellent chemical stability, high emission quantum yield and long lifetime of the excited state (15,(25)(26)(27)(28)(29)(30)(31)(32). The major limitations of ECL are those associated with electrodes, such as fouling, and the need for reconditioning of the electrode surface to sustain a constant CL signal.…”

Section: +mentioning

confidence: 99%

Chemiluminescence determination of chlorpheniramine using tris(1,10‐phenanthroline)–ruthenium(II) peroxydisulphate system and sequential injection analysis

et al. 2008

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] and a peristaltic pump to propel the sample. The experimental conditions affecting the chemiluminescence reaction were systematically optimized, using the univariate approach. Under the optimum conditions linear calibration curves of 0.1-10 mg/ml were obtained. The detection limit was 0.04 mg/ml and the relative standard deviation (RSD) was always < 5%. The procedure was applied to the analysis of CPA in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.

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Ru(bpy)32+-doped silica nanoparticle DNA probe for the electrogenerated chemiluminescence detection of DNA hybridization

Cited by 94 publications

References 33 publications

[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

Electrochemiluminescence detection of methamphetamine based on a Ru(bpy)₃²⁺‐doped silica nanoparticles/Nafion composite film modified electrode

Chemiluminescence determination of chlorpheniramine using tris(1,10‐phenanthroline)–ruthenium(II) peroxydisulphate system and sequential injection analysis

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Ru(bpy)32+-doped silica nanoparticle DNA probe for the electrogenerated chemiluminescence detection of DNA hybridization

Cited by 94 publications

References 33 publications

[Ru(bpy)3]2+‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

[Ru(bpy)3]2+‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

Electrochemiluminescence detection of methamphetamine based on a Ru(bpy)32+‐doped silica nanoparticles/Nafion composite film modified electrode

Chemiluminescence determination of chlorpheniramine using tris(1,10‐phenanthroline)–ruthenium(II) peroxydisulphate system and sequential injection analysis

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[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

[Ru(bpy)₃]²⁺‐Doped Silica Nanoparticles within Layer‐by‐Layer Biomolecular Coatings and Their Application as a Biocompatible Electrochemiluminescent Tag Material

Electrochemiluminescence detection of methamphetamine based on a Ru(bpy)₃²⁺‐doped silica nanoparticles/Nafion composite film modified electrode