Rotavirus excretion in naturally infected pigs with and without diarrhoea

Gatti, Maria Sílvia Viccari; Ferraz, Mirtis Maria Giaciani; Rácz, Maria Lucia; Castro, Antonio Fernando Pestana de

doi:10.1016/0378-1135(93)90193-b

Cited by 19 publications

(11 citation statements)

References 7 publications

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“…Lower incidences in suckling and fattening pigs correlate with other studies where weanling pigs were also found to be most often infected with rotaviruses (Gatti et al, 1993;Wieler et al, 2001). There is a limited number of comparable studies throughout the world that have looked for asymptomatic rotavirus infection in piglets.…”

Section: Discussionsupporting

confidence: 73%

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

116

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A surveillance of human, porcine and bovine rotaviruses was carried out in Slovenia in 2004 and 2005. Stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group A rotaviruses using RT-PCR and analysed by sequencing. The aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to detect reassortment among rotaviruses. The rates of asymptomatic shedding of rotaviruses in pigs and cattle were 18.0 % (67/373) and 4.0 % (5/126), respectively. Evidence for zoonotic transmission was detected in one human rotavirus strain, SI-MB6, with the G3P[6] genotype combination, as the nucleotide and predicted amino acid sequences of the VP6, VP7, VP8* and NSP4 genes of strain SI-MB6 and of porcine strains showed high nucleotide and amino acid sequence identity. Two porcine rotavirus strains carried VP7 of probable human origin, suggesting an interspecies reassortment event in the past.

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Section: Discussionsupporting

confidence: 73%

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer

Poljšak-Prijatelj

Barlič–Maganja

et al. 2008

Journal of General Virology

116

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“…In this study electropherotyping (RNA-PAGE) was selected as the primary method for detection of RV strains in diarrhoeic piglets, because each RV strain reveals a single distinct electropherotype upon PAGE and 11 segments of dsRNA by PAGE yield a pattern which is both constant and characteristic for a particular RV isolate [20]. Similar observations were also reported by earlier workers [21,22,23] Garam G.B., Bora D.P., Bora M., Ahmed S.P., Das S.K. and Malik Y.S.…”

Section: Resultssupporting

confidence: 61%

Detection and Genotypic Characterization of Rotavirus From Diarrhoeic Piglets of Arunachal Pradesh, A North Eastern State of India

Garam¹,

Bora²,

Bora³

et al. 2018

International Journal of Microbiology Research

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“…This clearly connotes that RV transmission from animal origin to human population is maximum in close setting. The discontinuous RNA-PAGE was employed for RV strain grouping and studies also reported that this technique is another mostly used gold standard test for RV detection in faeces [2,5,11,16,24]. Analysis of electrophoretic mobility of the 11 segments of RV dsRNA by PAGE corresponded to the basic pattern of 4:2:3:2 typical for group A rotavirus (RVA).…”

Section: Discussionmentioning

confidence: 99%

Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India

et al. 2013

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Rotavirus (RV) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. Human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. Therefore, the present study was designed to explore RV incidence in a single approach from human and animal neonates sharing similar environment. Altogether, 200 diarrheal samples from children (50), piglets (80) and calves (70) were collected during the year of 2010-2012 from various locality, farms and hospitals, initially screened through monoclonal antibody based enzyme immunoassay followed by RNA-PAGE and VP7 gene amplification by Reverse transcription PCR. The overall prevalence of rotavirus was found to be 41.5 % (83/200) where maximum numbers of positive cases were found in piglets (46.3 %) followed by human (40 %) and cow (37.1 %). Majority of samples demonstrated characteristic group A rotavirus (RVA) electropherotype of 4:2:3:2 pattern. Moreover, RNA profiles of seven samples from piglets and calves revealed variation in the migration pattern of class II, III and class IV segments. The study, for the first time from the valley, detected 43.7 % of neonatal RVA positive cases from human and animal sharing similar setting. The variation in RNA migration pattern in seven cases signifies tentative cases of gene re-assortment that warrant further evaluation.

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Rotavirus excretion in naturally infected pigs with and without diarrhoea

Cited by 19 publications

References 7 publications

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Detection and Genotypic Characterization of Rotavirus From Diarrhoeic Piglets of Arunachal Pradesh, A North Eastern State of India

Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India

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