Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer

Kamin, Ralph A.; Wilson, George S.

doi:10.1021/ac50058a010

Cited by 1,151 publications

(475 citation statements)

References 29 publications

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“…In agreement with the model developed by Savinell and coworkers [49], a non-linear Lineweaver-Burke plot (¿ _1 vs. substrate -1 ) is qualitatively indicative of a mass transport limitation, and that the operating potential suffices to elimínate the electrolysis rate from consideration, this behaviour corresponds to a sensor in which the substrate diffusion becomes slower than other reaction steps [50].…”

Section: Glucose Sensor Propertiessupporting

confidence: 85%

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Armada¹,

Losada²,

López-Villanueva

et al. 2008

Journal of Organometallic Chemistry

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The electrochemical characterization of three different polystyrene-b-polybutadiene block-copolymers, functionalized with diferrocenylsilane units, is reported. The PB-blocks have been functionalized with different fractions of electronically communicated, PS m -PB" p (HSiMeFc 2 ) p units, where m = 615, n = 53, p = 39 (1), m = 375, n = 92, p = 76 (2) and m = 455, n = 204, p = 170 (3). Electrochemical characterization has been carried out both in solution and after electrochemical deposition onto platinum electrodes. The bioelectrocatalytical properties of electrodes modified with the polymers in the nicotinamide dinucleotide (NADH) and glucose oxidase (GOx) oxidations have been investigated as a function of the constitution and structure of the polymers. The analytical properties of electrodes modified with these polymers as sensors of NADH and GOx are described. In addition, an amperometric biosensor for glucose, prepared by electrostatic immobilization of glucose oxidase onto a platinum electrode modified with one of the ferrocenyl block-copolymers as an example, has been developed.

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Section: Glucose Sensor Propertiessupporting

confidence: 85%

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Armada¹,

Losada²,

López-Villanueva

et al. 2008

Journal of Organometallic Chemistry

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“…The response of the electrode exhibits characteristics of the Michaelis-Menten kinetic mechanism. The apparent Michaelis-Menten constant, Km, of tyrosinase on the tyrosinase, NG-MTP-Au electrode to substrate can be calculated according to the Lineweaver-Burk equation, 9,[17][18][19] …”

Section: Amperometric Resultsmentioning

confidence: 99%

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

et al. 2006

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Many studies on the use of enzymatic activity in nonaqueous solutions have been carried out after Dastoli and Price's report of their investigation of the catalytic activity of xanthine oxidase suspended in several organic media. 1 Updike and Hicks published the results of their experiment involving an enzyme electrode with cross-linked polyacrylamide (PAA) membrane as matrix for use in aqueous solutions.2 Enzymes have been found to have numerous applications as practical and specific catalysts in chemical and pharmaceutical syntheses and as recognition elements in biosensors. The discovery that they are catalytically active in organic solvents containing little or no water has expanded their repertoire of use still further. 3,4 Some of the benefits in utilizing enzymes in nonaqueous solvents over the use of conventional aqueous reaction media include the high solubility of many hydrophobic substrates in such solvents, the suppression of various side reactions promoted by water, altered enzymatic selectivity and the ability to control it with the solvent, and the comparative simplicity of immobilization procedures due to the insolubility of enzymes in organic solvents. Many studies have been conducted in order to gain an understanding of so-called solvent effects due to organic solvent properties. Factors such as solvent hydrophobicity, 5 solvent polarity, water content, 6 and substrate hydrophobicity 7 can affect the enzymatic reaction. It remains important to continue the search for new enzymatic reaction systems because of the use which might be made of such systems in both pure and applied research.Many enzyme-based electrochemical biosensors have been described for use in both aqueous and nonaqueous solutions. A tyrosinase-immobilized polyacrylamide membrane platinum electrode has been prepared and the electrochemical behavior of o-hydroxybenzene derivatives in acetonitrile 8 and dimethylacetamide 9 has been studied. Because of unique properties of nanoparticles, recent investigations have focused on the development of enzyme-modified nanoparticle-based electrochemical biosensors. [10][11][12] The ability of nanoparticles to stabilize enzymes, and to improve the efficiency of immobilized enzymes is extremely useful when preparing biosensors. Moreover, other characteristics of nanoparticles, such as their high surface-to-volume ratio, their high surface energy, and their ability to decrease the distance between enzyme and metal particles, may facilitate electron transfer between redox sites and the electrode surfaces.In our laboratory, a gold nanoparticle self-assembled electrode has been constructed by using 4,4′-bis(methanethiol)biphenyl (MTP), rigid rod dithiols, as a binder between a gold disk and gold nanoparticles. The effect of gold nanoparticles on the interaction of Co(phen)3 3+ with DNA, and the characteristics of the electrode in a nonaqueous solution were described in a recent report. 13 We have also devised a method of preparing density-controlled gold nano-particle self-assembled monolayer (SAM...

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“…According to Michaelis-Menten kinetic mechanism, the K M app could be obtained from the electrochemical version of the Lineweaver-Burk equation. 45 1/I ss = 1/I max +K M app /(I max C)…”

Section: Effect Of Ph Valuementioning

confidence: 99%

Electrodeposition CaCO₃ Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor

Zhao

Zheng

Sheng

2011

J Chinese Chemical Soc

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By one-step co-electrodeposition CaCO 3 nanoparticles-chitosan composite film on carbon ionic liquid electrode (CILE), and then by spreading the composition of hemoglobin (Hb) and chitosan on the nanoCaCO 3 -chi/CILE, a Hb-chi/nanoCaCO 3 -chi/CILE was fabricated and the direct electrochemistry and electrocatalysis of Hb at the electrode was investigated. The electrochemical impedance spectroscopy of the modified electrode showed the electron transfer resistance was 1166 W. Investigation results of cyclic voltammetrys showed a pair of well-defined and quasireversible redox peak of Hb with the formal potentials of -0.295 V (vs. SCE) in 0.1 mol·L -1 pH 7.0 PBS; the response time of the reduction peak currents of Hb was lower than 3s; a linear range for determination of H 2 O 2 was from 5.0 mmol·L -1 to 1.3 mmol·L -1 with a detection limit of 1.6 mmol·L -1 (S/N = 3) and a sensitivity of 0.16 A·M -1 ·cm -2 ; the electron transfer rate constant and the apparent Michaelis-Menten constant of Hb were 1.98 s -1 and 0.81 mmol·L -1 , respectively. As a result, the case of the one-step co-electrodeposition and the promising feature of biocomposite could serve as a versatile platform for the fabrication of electrochemical biosensors.

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Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer

Cited by 1,151 publications

References 29 publications

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

Electrodeposition CaCO₃ Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor

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Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer

Cited by 1,151 publications

References 29 publications

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Electrochemical and bioelectrocatalytical properties of novel block-copolymers containing interacting ferrocenyl units

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

Electrodeposition CaCO3 Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor

Contact Info

Product

Resources

About

Electrodeposition CaCO₃ Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor