Electrodeposition CaCO₃ Nanoparticles‐chitosan Composite Film on Carbon Ionic Liquid Electrode as a Platform for Hemoglobin Electrochemical Biosensor

Abstract

By one-step co-electrodeposition CaCO 3 nanoparticles-chitosan composite film on carbon ionic liquid electrode (CILE), and then by spreading the composition of hemoglobin (Hb) and chitosan on the nanoCaCO 3 -chi/CILE, a Hb-chi/nanoCaCO 3 -chi/CILE was fabricated and the direct electrochemistry and electrocatalysis of Hb at the electrode was investigated. The electrochemical impedance spectroscopy of the modified electrode showed the electron transfer resistance was 1166 W. Investigation results of cyclic voltammetrys showed a pair of well-defined and quasireversible redox peak of Hb with the formal potentials of -0.295 V (vs. SCE) in 0.1 mol·L -1 pH 7.0 PBS; the response time of the reduction peak currents of Hb was lower than 3s; a linear range for determination of H 2 O 2 was from 5.0 mmol·L -1 to 1.3 mmol·L -1 with a detection limit of 1.6 mmol·L -1 (S/N = 3) and a sensitivity of 0.16 A·M -1 ·cm -2 ; the electron transfer rate constant and the apparent Michaelis-Menten constant of Hb were 1.98 s -1 and 0.81 mmol·L -1 , respectively. As a result, the case of the one-step co-electrodeposition and the promising feature of biocomposite could serve as a versatile platform for the fabrication of electrochemical biosensors.

Acetate chitosan with CaCO3 doping form tough hydrogel for hemostasis and wound healing

“…With the addition of the cross‐linker in the hydrogels, the storage modulus will increase due to the increased cross‐linking density of the hydrogel network . The reaction between H + and CaCO 3 generates free Ca 2+ ions, which as expected is effective at cross‐linking polymer .…”

“…Due to the electrostatic interactions between calcium (Ca 2+ ) and polymers, Ca 2+ has been reported as a cross‐linker to incorporate into the polymers, resulting in the formation of hydrogels . Some groups have dispersed CaCO 3 nanoparticles into amyloid fibrils or chitosan to strengthen the physical network, forming strong and stiff colloidal gels . Because of the advantages of CaCO 3 , in this study, a novel hydrogel (acetate chitosan/CaCO 3 , ACT/CaCO 3 ) was developed by simple complex acetate chitosan with CaCO 3 (Figure ).…”

“…26,27 Some groups have dispersed CaCO 3 nanoparticles into amyloid fibrils or chitosan to strengthen the physical network, forming strong and stiff colloidal gels. 28,29 Because of the advantages of CaCO 3 , in this study, a novel hydrogel (acetate chitosan/CaCO 3 , ACT/CaCO 3 ) was developed by simple complex acetate chitosan with CaCO 3 (Figure 1). There are 3 advantages of our formed hydrogel for wound healing.…”