Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements

Abstract

The study aimed at evaluating the response of common plum (Prunus domestica L.) microshoots during in vitro rooting in the presence of two phytoactive medium supplements, i.e. a dialyzate of pineapple pulp and a conditioned medium containing green algae Desmodesmus subspicatus exudates. Rooting efficiency was evaluated after 4 weeks of culture. During the root induction phase the content of phenolic compounds in shoot bases was determined and anatomical studies were conducted. Medium supplements were analyzed for the content of carbohydrates and phenolic acids. Both supplements were efficient in rooting induction of shoots of a difficult-to-root cultivar 'Węgierka Zwykła'. Medium supplementation allowed a significant reduction in the exogenous auxin content required for rooting. The highest rooting efficiencies on supplemented media were 28.9 and 27.8 %, in comparison with 33.3 % obtained in the control medium with doubled concentration of exogenous auxins. In the easy-to-root plum cultivar 'Węgierka Dąbrowicka' the rooting rate was slightly reduced in the presence of pineapple dialyzate, while in the presence of algal conditioned medium the rooting rate decreased substantially compared with the nonsupplemented medium. Approximately 30 % of 'Węgierka Dąbrowicka' shoots rooted on supplemented auxin-free media. The content of phenolic compounds accumulated in shoot bases during the root induction stage reflected the differences in rooting ability between both plum cultivars, indicating potential stressful conditions of the culture generated by the presence of phytoactive natural supplements. Anatomical study allowed to recognize the mode of dedifferentiation leading to adventitious rhizogenesis in the common plum. The results are discussed in relation to the composition of medium supplements and their potential root-promoting activity.

Morpho-anatomical and biochemical changes associated with rooting of micropropagated ninebark cuttings

“…The succession of anatomic changes during root development observed here was similar to that described for other woody plants, such as apple (Naija et al 2008), plume (Prunus domestica L.) (Wiszniewska et al 2016), chestnut (Castanea sativa Mill.) (Ballester et al 1999), oak (Quercus robur L.) (Vidal et al 2003) and smoke tree (Cotinus coggygria Scop.)…”

Humic Acid and Auxins Induced Metabolic Changes and Differential Gene Expression during Adventitious Root Development in Azalea Microshoots

“…It was reported that ferulic, gallic, and chlorogenic acid could enhance woody plant rooting (De Klerk et al, 2011). In addition, the levels of endogenous phenolics were considered as well-defined physiological indicators related to adventitious root formation (Curir et al, 1990;de Klerk et al, 1999;Wiszniewska et al, 2016). In this experiment, we deserved that the total phenols, flavonols, polyphenolic acids, and anthocyanins in the studied plants increased in all treatments compared with those in the control until day 21 and then decreased at day 28.…”

“…They are also considered one of the defense groups that inhibit stresses caused by wounding or adverse environmental conditions (Solar et al, 2006). However, the mechanisms of action of phenolic compounds and their accumulation during the induction of adventitious roots are not quite clear (Wiszniewska et al, 2016). Changes in carbohydrate levels have vital roles in plant developmental, physiological, and metabolic processes involved in plant formation (Mishra et al, 2009).…”

Propagation in Vitro and Experimental Field Cultivation ex Situ of Pulsatilla Turczaninovii Kryl. et Serg. (Ranunculaceae)

“…Rooting di culties limit the rate of vegetative propagation of the species, and also are a serious obstacle in its commercial propagation. In order to improve this stage of plantlet development, an insight into rooting physiology of di cult-to-root species is necessary (Wiszniewska et al 2016). The changes in accumulation of phenolic compounds and carbohydrates, as well as in activity of peroxidase are often associated with rooting ability and root formation at the earliest stages of rhizogenesis (de Klerk et al 1999, Günes 2000, Denaxa et al 2012.…”

“…The changes in accumulation of phenolic compounds and carbohydrates, as well as in activity of peroxidase are often associated with rooting ability and root formation at the earliest stages of rhizogenesis (de Klerk et al 1999, Günes 2000, Denaxa et al 2012. Monitoring of their uctuations may allow choosing the most optimal time for plantlet transfer to elongation medium, adjusted to altered auxin requirements of cultured plantlets (de Klerk et al 1999, Wiszniewska et al 2016. We observed that in P. turczaninovii rooting parameters improved when accumulation of soluble sugars was enhanced, similarly to results reported for carnation cuttings (Agulló-Antónet al 2011).…”