Morpho-anatomical and biochemical changes associated with rooting of micropropagated ninebark cuttings

Abstract

Ninebark (Physocarpus opulifolius) is an attractive ornamental shrub with poor rooting characteristics in some cultivars, which is a limiting factor in commercial production This study was designed to optimize rooting conditions of ninebark cuttings and to observe the effect of exogenous auxin IBA on some morpho-anatomical and biochemical changes associated with rhizogenesis in the in vitro conditions. Both auxins under study: the indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) gave comparable effects but the combination of ½ MS + 1 mg·L−1 IBA was the most cost effective for all rooting parameters. Anatomical changes at the cuttings’ bases during root formation were typical for woody plants and they were accelerated by auxin in the culture medium. High levels of the endogenous indole acid and hydrogen peroxide were temporarily associated with intensive cell divisions in cuttings, and the polyphenolic acid contents kept increasing during rooting above the initial levels and those in controls.

henolics profile and antioxidant activities of in vitro propagules and field-raised plant organs of Curculigo latifolia

“…It is generally accepted that phenolic compounds act synergistically with auxin in the developing callus and organs. A high level of phenolics protects auxin from O n l i n e F i r s t oxidation, making it more effective (Jagiełło-Kubiec et al, 2021). The environmental condition and plant growth stage influence the secondary metabolite content and composition (Yeshi et al, 2022).…”