Curculigo orchioides and C. latifolia have been used as traditional medicines such as antidiabetic and anticancer. This study measured the total phenolics and flavonoid contents as well as analyzed the functional groups and chemical compounds using Fourier-transform infrared (FTIR) spectra and UHPLC-Q-Orbitrap-HRMS profiling for the discrimination of plant parts, geographical origin, and compounds that presumably have a significant contribution as antioxidant and α-glucosidase inhibitors on both plants. The total phenolics and flavonoids contents in Curculigo species varied from 142.09 to 452.47 mg gallic acid equivalent (GAE/g) and from 0.82 to 5.44 mg quercetin equivalent (QE/g), respectively. The lowest IC50 for antioxidant and α-glucosidase inhibitory activities is presented by C. latifolia from a higher altitude region. Principal component analysis (PCA) from FTIR and UHPLC-Q-Orbitrap-HRMS data could discriminate the plant parts and geographical origin. Partial least squares (PLS) analysis has identified several functional groups, such as O–H, C–H, C=O, C–C, C–O, and chemical compounds, unknown-185 and unknown-85, that are most likely to contribute to the antioxidant and α-glucosidase inhibitory activities.
Curculigo orchioides and Curculigo latifolia (Hypoxidaceae) have been widely used as traditional medicines in Indonesia and other Asian countries for antihyperglycemic, aphrodisiac, antioxidant, and antimicrobial treatments. This work aimed to determine the distribution of secretory structures and metabolites. Metabolite profiling approach of the plant organs was determined by UHPLC-Q-Orbitrap-HRMS. Histochemical-based techniques on rhizome, root, petiole, and leaf, with transversal sections on fresh samples, were prepared using a razor blade to determine the secretory structures of the plants, followed by plant anatomy procedures. Histochemical analysis was carried out using several reagents to detect the metabolites. Metabolite profiling revealed several classes of compounds, i.e. phenolics, alkaloids, terpenes, essential oils, and lipophilic. Secretory cavities and idioblast cells, were detected in both species and localize a diverse metabolites. Additionally to the specialized structures, hypodermis, epidermis, intercellular spaces, and cuticle also contain some of those compounds. The secretory structures spread over the different organs. This discovery may be useful to distinguish particular organs as medicine source, which does not only depend on the availability of rhizome. The secretory structures and the chemical compounds of the two species, described herein for the first time, can be used further in plant identification purposes based on chemical markers.
This research was aimed at evaluating the pH of beef, as well as its cook loss value and water holding capacity, as well as its proximate composition of the beef from the Kano State abattoir. The proximate composition of the beef was also analyzed using standard procedures described by the Association of the Official Analytical Chemists (AOAC). The beef samples were aseptically obtained from the Kano state abattoir during the month of September when the animals were well fed and healthy, with five cows randomly selected and used for the analysis. The results indicate the pH for the beef to be 5.9, while the cook loss and water-holding capacity were found to be 32.71% and 24.33% respectively. The proximate contents were also reported as follows; protein content (21.50%); fat content (6.75%); ash content (0.92%) and moisture content (68.19%). The results in indicate that all the parameters analyzed for the beef obtained from the Kano abattoir are comparable with the results reported by various researchers, and are all within the standard acceptable limits. Consequently, this impart positive economic implications to the both seller and the consumer, as well as enhancing the meats taste and palatability. The present study concluded that the meat from the Kano State abattoir can be safely consumed by the consumers.
Mulberry (Morus alba L.) leaf has been used as a traditional medicine for treatment of some tropical diseases and also popular using for silkworm feed. On this research, anti-inflammatory and wound healing activities of the bark extract has been investigated in vivo. The bark was extracted with three organic solvent in a different polarity to carry out a group compounds in n-hexane extract (EH), Eto-Ac extract (EE), and EtOH extract (EO). Anti-inflammatory was investigated on inflammatory mice induced by 1% carrageenan. Among the extract (200mg/kg BW), EE showed strongly decrease inflammation by 42% than the other extract and stronger than the positive control, Na-diclofenac. Further investigation on healing effect of wound and burn injury which was tested at concentration of 1% topically to each extract. All the extract significantly showed healing activity, among them EE was observed as the stronger one while slightly less than the positive control. This result indicated that the most active compounds on anti-inflammatory and healing wounded was extracted with ethyl acetate that could be used for drug discovery.
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