Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

Abstract

bcde Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, userfriendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS-paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

Perovskite-Inspired Lead-Free Ag2BiI5 for Self-Powered NIR-Blind Visible Light Photodetection

“…Visible light photodetection is central to many emerging optoelectronic applications (e.g., wearable optoelectronics [1], lab-on-chip and biomedicine [2,3], and the Internet of Things (IoT) [4]). Emerging solution-processed semiconductors such as perovskite or perovskite-inspired semiconductors [5][6][7][8][9] are highly attractive to this end, as they are compatible with unconventional substrates and facile, lowcost manufacturing.…”

Efficiency and spectral performance of narrowband organic and perovskite photodetectors: a cross-sectional review

“…Within the visible range (wavelength λ≈400-740 nm), this functionality has been conventionally employed to determine the colour of light with respect to a set of primaries (e.g., three colour bands of ≈100-150 nm width), as needed for colorimetry and digital colour photography, for example. This is now highly sought after for a number of growing or emerging areas, e.g., the Internet of Things (IoT), artificial intelligence, computer vision, biosensing [1][2][3]. Many such emerging areas require tighter performance, e.g., often demanding spectral widths of 100 nm or less [4,5], and significantly smaller (down to ≈10 nm) for multispectral and hyperspectral detection/ imaging.…”

LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

“…The probe's fluorescence was detected using a hand-held ultraviolet source, achieving an LOD of z5 cells. Roll-to-roll thermal imprinting (molding) technology was proposed for the fabrication of integrated PDMSepaper microfluidic device for molecular diagnostics [53]. This technology enabled the scaling up of production to thousands of devices in an hour (Fig.…”