LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

Abstract

a b s t r a c tNucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is simpler in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.

Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

“…SENSR can be implemented in any diagnostic laboratory with commonly available instruments such as a water bath and a spectrophotometer. In addition, the full potential of SENSR would be realized through the development of field-deployable devices for isothermal incubation and fluorescence measurement 37,[40][41][42][43] . Due to the simplicity of the operating mechanism, SENSR can be further developed into a portable, easy-to-use test of paper-based or lateral-flow type, as exemplified by recent developments in nucleic acid diagnostics 9,[44][45][46][47] .…”

Recent advances in lab-on-a-chip technologies for viral diagnosis

“…Isothermal NA amplification methods (Zanoli and Spoto, 2013;Zhang et al, 2019) are carried out at a constant temperature, typically between �37 � C and �65 � C, making the isothermal amplification system hardware simpler than the one used for PCR. Many isothermal amplification methods have been reported and can be grouped based on the reaction principle.…”

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

“…Loop-mediated isothermal amplification (LAMP) is another promising technique to quickly preconcentrate nucleic acids developed by Notomi et al in 2000 [156]. The advantage of LAMP over traditional PCR methods is the completion at a single set temperature (approximately 65 • C) within a single tube [157,158]. It involves a three-step process starting with sample material priming, amplification, and then elongation and further exponential amplification.…”