Roles of the Aspergillus nidulans UDP-galactofuranose transporter, UgtA in hyphal morphogenesis, cell wall architecture, conidiation, and drug sensitivity

UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose

“…These results are similar to those that have been reported for bacteria, demonstrating that EPS can protect bacteria against the anionic hydrophobic agent SDS (Liao et al, 2014). In our study, we found that the ugpi strains had thicker and mostly electron-transparent cell walls, similar to results that have been reported for an Aspergillus nidulans UDP-sugar transporter mutant (Afroz et al, 2011). However, these results differed from those of a study of A. thaliana reporting a cell wall defect in an atugp1/atugp2 another double mutant (Chen et al, 2007).…”

Putative Calcium Channels CchA and MidA Play the Important Roles in Conidiation, Hyphal Polarity and Cell Wall Components in Aspergillus nidulans

“…Scanning electron microscopy examination was carried out mainly as described previously [38], [39], [40]. In brief, for sample preparation, sporulating colonies that had grown on MMPDR for 2 d at 37°C were fixed firstly for 2 h in 4% glutaraldehyde with 0.01 M phosphate buffer (pH 7.0) at room temperature, then washed by 0.01 M phosphate buffer (pH 7.0).…”

Overlapping and Distinct Roles of Aspergillus fumigatus UDP-glucose 4-Epimerases in Galactose Metabolism and the Synthesis of Galactose-containing Cell Wall Polysaccharides

“…Indeed, sugar epimerase have important functions outside of cell wall polysaccharide synthesis, such as metabolism. Redundancy in hexose interconversion would ensure that the organism can adapt to different carbon sources and maintain glycolysis and generate derivatives required for other cellular activities (12,13,(61)(62)(63). In contrast, GalNAc seems to be required by A. fumigatus primarily for the synthesis of galactosaminogalactan and is not required for normal growth (22).…”

“…UDP-galactopyranose is in turn modified to UDP-galactofuranose through the activity of the UgmA, an UDP-glucose mutase. UDP-galactofuranose is then transported by the glycosyl transporter, UgtA (13), into the Golgi where the glycosylation of carbohydrates, proteins, and lipids occurs. Although the glycosyltransferases involved in chain formation remain unknown in Aspergillus, galactofuranosyltransferases have been studied in bacteria (14 -17) and protozoans (18).…”