Roles of methionine and cysteine residues of the <i>Escherichia coli</i> sensor kinase <scp>HprS</scp> in reactive chlorine species sensing

Yamaji, Kotaro; Taniguchi, Rumine; Urano, Hiroyuki; Ogasawara, Hiroshi

doi:10.1002/1873-3468.14574

Cited by 3 publications

(2 citation statements)

References 48 publications

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“…For the chlorate biosensor we present in this study, we used the bacteria Escherichia coli . The sensing module comprises an HprSR two-component system, which is triggered by the oxidation of methionine residues located in the periplasmic domain of HprS [ 20 , 26 ]. Meanwhile, the reporting module is represented by the expression level of the hiuH-msrPQ operon.…”

Section: Discussionmentioning

confidence: 99%

Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

et al. 2023

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Chlorate can contaminate food due to the use of chlorinated water for processing or equipment disinfection. Chronic exposure to chlorate in food and drinking water is a potential health concern. The current methods for detecting chlorate in liquids and foods are expensive and not easily accessible to all laboratories, highlighting an urgent need for a simple and cost-effective method. The discovery of the adaptation mechanism of Escherichia coli to chlorate stress, which involves the production of the periplasmic Methionine Sulfoxide Reductase (MsrP), prompted us to use an E. coli strain with an msrP-lacZ fusion as a biosensor for detecting chlorate. Our study aimed to optimize the bacterial biosensor’s sensitivity and efficiency to detect chlorate in various food samples using synthetic biology and adapted growth conditions. Our results demonstrate successful biosensor enhancement and provide proof of concept for detecting chlorate in food samples.

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Section: Discussionmentioning

confidence: 99%

Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

et al. 2023

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“…This is supported by the fact that N279H is the only mutant to not over express hprR/S and hiuH and as a result in DMB, show low efflux pump expression as compared to the N-terminal mutants. Both HprR and CusR have also been shown to collaborate to induce expression of hiuH which is also overexpressed in the three N-terminal mutants (Urano et al, 2015;Yamaji et al, 2023). Crosstalk between these systems may be able to help prepare the cell for increased oxidative stress that would be generated by excess metals in the cell via Fenton reactions (Urano et al, 2015).…”

Section: Evidence Supporting the Maintenance And Occurrence Of R15l I...mentioning

confidence: 99%

Genotype-by-environment interactions govern fitness changes associated with adaptive mutations in two-component response systems

Sanders,

Miller,

Ahmidouch

et al. 2024

Front. Genet.

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Introduction: Two-component response systems (TCRS) are the main mechanism by which prokaryotes acclimate to changing environments. These systems are composed of a membrane bound histidine kinase (HK) that senses external signals and a response regulator (RR) that activates transcription of response genes. Despite their known role in acclimation, little is known about the role TCRS play in environmental adaptation. Several experimental evolution studies have shown the acquisition of mutations in TCRS during adaptation, therefore here we set out to characterize the adaptive mechanism resulting from these mutations and evaluate whether single nucleotide changes in one gene could induce variable genotype-by-environment (GxE) interactions.Methods: To do this, we assessed fitness changes and differential gene expression for four adaptive mutations in cusS, the gene that encodes the HK CusS, acquired by Escherichia coli during silver adaptation.Results: Fitness assays showed that as the environment changed, each mutant displayed a unique fitness profile with greatest fitness in the original selection environment. RNAseq then indicated that, in ± silver nitrate, each mutant induces a primary response that upregulates cusS, its RR cusR, and constitutively expresses the target response genes cusCFBA. This then induces a secondary response via differential expression of genes regulated by the CusR through TCRS crosstalk. Finally, each mutant undergoes fitness tuning through unique tertiary responses that result in gene expression patterns specific for the genotype, the environment and optimized for the original selection conditions.Discussion: This three-step response shows that different mutations in a single gene leads to individualized phenotypes governed by unique GxE interactions that not only contribute to transcriptional divergence but also to phenotypic plasticity.

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TetR and OmpR family regulators in natural product biosynthesis and resistance

Patil,

Sharma,

Bhaskarwar

et al. 2023

Proteins

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This article provides a comprehensive review and sequence‐structure analysis of transcription regulator (TR) families, TetR and OmpR/PhoB, involved in specialized secondary metabolite (SSM) biosynthesis and resistance. Transcription regulation is a fundamental process, playing a crucial role in orchestrating gene expression to confer a survival advantage in response to frequent environmental stress conditions. This process, coupled with signal sensing, enables bacteria to respond to a diverse range of intra and extracellular signals. Thus, major bacterial signaling systems use a receptor domain to sense chemical stimuli along with an output domain responsible for transcription regulation through DNA‐binding. Sensory and output domains on a single polypeptide chain (one component system, OCS) allow response to stimuli by allostery, that is, DNA‐binding affinity modulation upon signal presence/absence. On the other hand, two component systems (TCSs) allow cross‐talk between the sensory and output domains as they are disjoint and transmit information by phosphorelay to mount a response. In both cases, however, TRs play a central role. Biosynthesis of SSMs, which includes antibiotics, is heavily regulated by TRs as it diverts the cell's resources towards the production of these expendable compounds, which also have clinical applications. These TRs have evolved to relay information across specific signals and target genes, thus providing a rich source of unique mechanisms to explore towards addressing the rapid escalation in antimicrobial resistance (AMR). Here we focus on the TetR and OmpR family TRs, which belong to OCS and TCS, respectively. These TR families are well‐known examples of regulators in secondary metabolism and are ubiquitous across different bacteria, as they also participate in a myriad of cellular processes apart from SSM biosynthesis and resistance. As a result, these families exhibit higher sequence divergence, which is also evident from our bioinformatic analysis of 158 389 and 77 437 sequences from TetR and OmpR family TRs, respectively. The analysis of both sequence and structure allowed us to identify novel motifs in addition to the known motifs responsible for TR function and its structural integrity. Understanding the diverse mechanisms employed by these TRs is essential for unraveling the biosynthesis of SSMs. This can also help exploit their regulatory role in biosynthesis for significant pharmaceutical, agricultural, and industrial applications.

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Roles of methionine and cysteine residues of the Escherichia coli sensor kinase HprS in reactive chlorine species sensing

Cited by 3 publications

References 48 publications

Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

Genotype-by-environment interactions govern fitness changes associated with adaptive mutations in two-component response systems

TetR and OmpR family regulators in natural product biosynthesis and resistance

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