Role of water‐soluble polysaccharides in bacterial cellulose production

Ishida, Takehiko; Mitarai, Makoto; Sugano, Yasushi; Shoda, Makoto

doi:10.1002/bit.10690

Cited by 50 publications

(29 citation statements)

References 18 publications

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“…As other polymeric additives such as agar, acetan, and xanthan, CMC exhibited its ability to hinder formation of large clumps of BC and enhanced BC production during submerged fermentation (Ishida et al 2003;Bae et al 2004;Zhou et al 2007). This can possibly be attributed to an increase in the solubility of BC in the presence of the highly soluble CMC, which may bind to the surface of the BC fibrils.…”

Section: Bc Structure and Physical Propertiesmentioning

confidence: 99%

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Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

2009

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Bacterial cellulose (BC) demonstrates unique properties including high mechanical strength, high crystallinity, and high water retention ability, which make it an useful material in many industries, such as food, paper manufacturing, and pharmaceutical application. In this study, different additives including agar, carboxymethylcellulose (CMC), microcrystalline cellulose, and sodium alginate were added into fermentation medium in agitated culture to enhance BC production by Acetobacter xylinum. The optimal additive was chosen based on the amount of BC produced. The produced BC was analyzed by using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), and dynamic mechanical analysis (DMA). Among the evaluated additives, CMC yielded highest BC production (8.2 g/L) compared to the control (1.3 g/L). The results also indicated that CMC-altered BC production increased with CMC addition and reached saturation around 1%. The variation between replicates for all analysis was \5%. From XRD analysis, however, the crystallinity and crystal size decreased as CMC addition increased. FESEM results showed CMC-altered BC produced from agitated culture retained its interweaving property. TGA results demonstrated that CMC-altered BC had about 98% water retention ability, which is higher than BC pellicle produced with static culture. CMC-altered BC also exhibited higher T max compared to control. Finally, DMA results showed that BC from agitated culture loses its mechanical strength in both stress at break and Young's modulus when compared to BC pellicle. This study clearly demonstrated that addition of CMC enhanced BC production and slightly changed its structure.

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Section: Bc Structure and Physical Propertiesmentioning

confidence: 99%

“…Water content of CMC-altered BC pellicle also increased from 73 to 96% (w/w). Addition of other water soluble polysaccharides such as agar, sodium alginate was also reported to enhance BC production in agitated cultivation (Ishida et al 2003;Bae et al 2004;Zhou et al 2007).…”

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confidence: 97%

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

2009

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“…Of all, adding appropriate stimulatory factors has received much attention for its simplicity and already observed significant effects on BC production. At present, studies on stimulatory factors mainly focus on ethanol (Naritomi et al 1998a), organic acids (Embuscado et al 1994;Toda et al 1997;Naritomi et al 1998b), Water-Soluble Polysaccharides (Bae et al 2004;Ishida et al 2003), and lignin sulfonate (Keshk and Sameshima 2006). Among them, ethanol has been shown to be a good stimulatory factor for BC production, and the underlying mechanism has also been investigated (Yunoki et al 2004).…”

Section: Introductionmentioning

confidence: 97%

Effects of alcohols on bacterial cellulose production by Acetobacter xylinum 186

Zhang

Chi

et al. 2011

World J Microbiol Biotechnol

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To improve the yield of cellulose production in bacteria, we investigated the stimulatory effects of six different alcohols during fermentation of Acetobacter xylinum 186. Our study showed that after static fermentation at 30°C for 6 days, bacterial culture with 1.0% (v/v) of methanol added in the medium produced the highest bacterial cellulose (BC) yield at 103.5 mg/100 ml, which was 21.8% higher than the control group. Addition of 0.5% of ethylene glycol in the culture yielded 105.5 mg/100 ml BC, 24.1% higher than the control group. Adding 0.5% of n-propanol yielded 96.4 mg/100 ml BC, 13.4% higher; 3.0% of glycerol yielded 108.3 mg/100 ml BC, 27.4% higher; 0.5% of n-butanol yielded 132.6 mg/100 ml BC, 56.0% higher; and 4.0% of mannitol in the culture yielded 125.2 mg/100 ml BC, 47.3% higher, respectively. The rate of bacterial cellulose production increased with the growth rate of the bacteria. The stimulatory effects of these alcohols that we observed were significant in the later stage of fermentation, which was considered to be important for the biosynthesis of bacterial cellulose.

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“…At Shoda laboratory at the Tokyo Institute of Technology, it was reported that BC productivity was 5.1 g/L in a 50 L Internal-Loop Airlift Reactor using Corn steep liquor-fructose (CSL-Fru) added with agar for 67 hours culture [12]. And Chao etc.…”

Section: Productivity Of Bc Depending Upon the Culture Modesmentioning

confidence: 99%

Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor

Choi

Song

Kim

et al. 2009

Korean J. Chem. Eng.

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The saccharogenic liquid obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes (SFW) was carried out by the cultivation supernatant of Trichoderma inhamatum KSJ1. 5.6 g/L of BC was produced in a new modified 50 L bubble column bioreactor by Acetobacter xylinum KJ1. The productivity was similar to that of a modified 10 L bubble column bioreactor (5.8 g/L). When pure oxygen was supplied into the scaled-up culture conditions, 6.8 g/L (12% enhancement) of BC was produced, indicating a very useful method for BC mass production. The oxygen uptake rate (OUR) and q O2 (specific oxygen uptake rate) were 0.214 mg-DO/L·min and 0.257 mg-DO/g-cell·min, respectively. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BCs produced in static culture (A), 10 L (B) and 50 L (C) modified bubble column cultures were investigated. All BCs showed fibrils with highly networking structure. The number average molecular weight of BCs in A, B and C was 2,314,000, 1,878,000, and 1,765,000, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 79.6-86.0%. Tensile strengths of BC sheet in A, B and C were 1.75, 1.21, and 1.19 kg/mm 2 , respectively. In conclusion, BC production by the modified bubble column culture mode of 50 L brought more favorable results in terms of the physical properties and its ease of scale-up.

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Role of water‐soluble polysaccharides in bacterial cellulose production

Cited by 50 publications

References 18 publications

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property

Effects of alcohols on bacterial cellulose production by Acetobacter xylinum 186

Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor

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