Effects of alcohols on bacterial cellulose production by Acetobacter xylinum 186

Abstract

To improve the yield of cellulose production in bacteria, we investigated the stimulatory effects of six different alcohols during fermentation of Acetobacter xylinum 186. Our study showed that after static fermentation at 30°C for 6 days, bacterial culture with 1.0% (v/v) of methanol added in the medium produced the highest bacterial cellulose (BC) yield at 103.5 mg/100 ml, which was 21.8% higher than the control group. Addition of 0.5% of ethylene glycol in the culture yielded 105.5 mg/100 ml BC, 24.1% higher than the control group. Adding 0.5% of n-propanol yielded 96.4 mg/100 ml BC, 13.4% higher; 3.0% of glycerol yielded 108.3 mg/100 ml BC, 27.4% higher; 0.5% of n-butanol yielded 132.6 mg/100 ml BC, 56.0% higher; and 4.0% of mannitol in the culture yielded 125.2 mg/100 ml BC, 47.3% higher, respectively. The rate of bacterial cellulose production increased with the growth rate of the bacteria. The stimulatory effects of these alcohols that we observed were significant in the later stage of fermentation, which was considered to be important for the biosynthesis of bacterial cellulose.

Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

“…Similarly, the addition of agar at a concentration of 0.6% in the stirred-tank reactor generated 11.6 g L − 1 of BC by Acetobacter xylinum BPR2001 [49]. In the present study, addition of 1% PEG 6000 as polymer additive in HS medium enhanced the BC yield as reported earlier [28,50].…”

Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus

“…It seems that butanol was a more suitable carbon source than ethanol for BC production by G. xylinus in the ABE fermentation wastewater. In other researches, both ethanol and butanol have been shown that could improve the BC yield (Yunoki et al 2004;Lu et al 2011).…”

Insights into Bacterial Cellulose Biosynthesis from Different Carbon Sources and the Associated Biochemical Transformation Pathways in Komagataeibacter sp. W1

“…More often, both ethanol and acetate are spiked in media to enhance BC production by improving adenosine triphosphate (ATP) production, which is responsible for energy supply in the tricarboxylic acid (TCA) cycle and sugar metabolisms. Specifically, these processes are achieved by promoting the activities of glucokinase and fructokinase for BC production and inhibiting the activities of gluconokinase and glucose-6-phosphate dehydrogenase in pentose phosphate metabolism for energy production [17,18,19,20,21,22].…”