Role of the SPS Gene Families in the Regulation of Sucrose Accumulation in Sugarcane

Huang, Dongliang; Qin, Cui-Xian; Gui, Yiyun; Zhao, Lingna; Chen, Zhongliang; Wang, Miao; Sun, Yue; Liao, Qing; Li, Yang–Rui; Lakshmanan, Prakash

doi:10.1007/s12355-016-0454-x

Cited by 12 publications

(11 citation statements)

References 19 publications

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“…6 ). In sugarcane, Huang et al (2017) found the intensity of sucrose synthesis is controlled by regulating SPSC and SPSA genes expression. The role of SPS C in tobacco is postulated to participate in sucrose synthesis in the phase of starch mobilisation at night ( Gibon et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

“… Castleden et al (2004) carried out phylogenetic analysis of all the known SPS genes and found that they could cluster into four distinct families: A, B, C, and D. Family D is only found in the Poaceae ( Castleden et al, 2004 ; Lutfiyya et al, 2007 ; Huang et al, 2017 ). Our results support plant SPS genes could be divided into four groups ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…LcSPS3 and LcSPS4 were clustered into B and C families, respectively, indicating there is at least one SPS gene from each family. In the previous studies, it was shown that the expression patterns and functional features of SPS genes vary among different families from different plants ( Reimholz et al, 1997 ; Huang et al, 2017 ). LcSPS1 - 4 gene belonged to different family, suggesting functional divergence.…”

Section: Discussionmentioning

confidence: 99%

“…OsSPS1 in rice belonging to B family was also reported to be present in source tissues, particularly in leaf blades ( Chávez-Bárcenas et al, 2000 ; Okamura et al, 2011 ). However, the expression level of SofSPSB (family B) in sugarcane is negligible in fully expanded leaves, indicating that SofSPSB is not involved in sucrose synthesis in source leaf ( Huang et al, 2017 ). Further study is needed to investigate the expression patterns of B family SPS genes in more plant species to figure out whether it could be used as a source-specific gene.…”

Section: Discussionmentioning

confidence: 99%

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Identification and expression profile analysis of the sucrose phosphate synthase gene family inLitchi chinensisSonn.

Wang

Zhao

et al. 2018

PeerJ

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Sucrose phosphate synthase (SPS, EC 2.4.1.14) is a key enzyme that regulates sucrose biosynthesis in plants. SPS is encoded by different gene families which display differential expression patterns and functional divergence. Genome-wide identification and expression analyses of SPS gene families have been performed in Arabidopsis, rice, and sugarcane, but a comprehensive analysis of the SPS gene family in Litchi chinensis Sonn. has not yet been reported. In the current study, four SPS gene (LcSPS1, LcSPS2, LcSPS3, and LcSPS4) were isolated from litchi. The genomic organization analysis indicated the four litchi SPS genes have very similar exon-intron structures. Phylogenetic tree showed LcSPS1-4 were grouped into different SPS families (LcSPS1 and LcSPS2 in A family, LcSPS3 in B family, and LcSPS4 in C family). LcSPS1 and LcSPS4 were strongly expressed in the flowers, while LcSPS3 most expressed in mature leaves. RT-qPCR results showed that LcSPS genes expressed differentially during aril development between cultivars with different hexose/sucrose ratios. A higher level of expression of LcSPS genes was detected in Wuheli, which accumulates higher sucrose in the aril at mature. The tissue- and developmental stage-specific expression of LcSPS1-4 genes uncovered in this study increase our understanding of the important roles played by these genes in litchi fruits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification and expression profile analysis of the sucrose phosphate synthase gene family inLitchi chinensisSonn.

Wang

Zhao

et al. 2018

PeerJ

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“…The methods described by (Chen 2017(Chen , 2019) and Huang 2017 were used for ScSuSy1 and ScSuSy4 expression analysis. RNA for qPCR was extracted from sampled tissues using Spin Column Plant total RNA Purification Kit (Songon Biotech, Shanghai, China).…”

Section: Expression Analysis Of Scsusy1 and Scsusy4 Genesmentioning

confidence: 99%

Sucrose Synthase Genes Showed Genotype-Dependent Expression in Sugarcane Leaves in the Early Stage of Growth

Chen¹,

Gui²,

Wang³

et al. 2021

IJAB

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Sucrose synthase (SuSy) is one of the key enzymes regulating sucrose metabolism in plants. There are at least five different SuSy genesexist in sugarcane, and their biological functions are not fully understood. In this paper, the phylogenetic analysis of all plantSuSygenes published to-date (109) has been grouped into three classes: SuSyI, SuSyII and SuSyIII. SuSyIwas further divided into monocot and eudicot SuSyI, indicating their independent evolutionary trajectory paralleling monocot and dicot divergence. The leaf total sugar content of high-, medium-and low-sugar sugarcane genotypes varied at the early stage of plant growth. However, there was no strong correlation between early-stage leaf sucrose content and the final stem sugar content and sugar yield of mature crop. The proportion of leaf sucrose and fructose content in most sugarcane genotypes in the early stage of growth was about 30%each, and that of glucose was about 40%. The SuSy enzyme activity and gene expression of sugarcane sucrose synthase gene ScSuSy1and ScSuSy4in those genotypes also showed a remarkable variation during the same growth period. The expression profiles of these genes in high-sugar, medium-and low-sugar genotypes were complex with no clear association between their activity at the early stage of plant growth and final sugar yield of those genotypes. The biological role of ScSuSy1and ScSuSy4gene may change with sugarcane plant development, and may be involved in both the synthesis and decomposition of sucrose.© 2021 Friends Science Publishers

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Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source–sink perturbed conditions

et al. 2017

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Role of the SPS Gene Families in the Regulation of Sucrose Accumulation in Sugarcane

Cited by 12 publications

References 19 publications

Identification and expression profile analysis of the sucrose phosphate synthase gene family inLitchi chinensisSonn.

Identification and expression profile analysis of the sucrose phosphate synthase gene family inLitchi chinensisSonn.

Sucrose Synthase Genes Showed Genotype-Dependent Expression in Sugarcane Leaves in the Early Stage of Growth

Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source–sink perturbed conditions

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