Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source–sink perturbed conditions

Verma, I.; Roopendra, K.; Sharma, Amita; Jain, Rajni; Singh, Ravinder; Chandra, Amaresh

doi:10.1007/s11738-017-2433-6

Cited by 16 publications

(11 citation statements)

References 63 publications

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“…About 120 uniform plants were maintained in each plot and a solution (35 ppm) of GA 3 (Moore ; Verma et al . ) was applied on upper parts of the stalk using a battery‐operated uniform controlled dispensing sprayer. Control plants (drought‐stressed) were sprayed only with distilled water (no GA 3 solution).…”

Section: Methodsmentioning

confidence: 99%

“…; Verma et al . ). Recently, GA 3 has also been proposed to improve tolerance to environmental stresses (Iqbal et al .…”

Section: Introductionmentioning

confidence: 97%

“…Application of GA 3 induces invertase activity, which breaks down sucrose into glucose and fructose, thus increased available hexoses that can easily be transported to sink tissues and increase sink strength (Verma et al . ). Iqbal et al .…”

Section: Introductionmentioning

confidence: 97%

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Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage

2018

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Drought is one of the most serious environmental factors limiting production of sugarcane worldwide. In order to assess the influence of gibberellins (GA 3 ) on drought and plant growth, along with associated physio-biochemical attributes, expression of eight drought-responsive genes were quantified and analysed.• Results demonstrated that exposure of droughted plants to GA 3 not only led to recovery of activity of drought-associated physio-biochemical attributes, but also minimised impact on cane dry weight and quality. Further, GA 3 application caused differential gene expression that possibly triggers increased responsiveness towards drought tolerance in sugarcane.

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Section: Methodsmentioning

confidence: 99%

“…; Verma et al . ). Recently, GA 3 has also been proposed to improve tolerance to environmental stresses (Iqbal et al .…”

Section: Introductionmentioning

confidence: 97%

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Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage

2018

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“…Cell wall invertase plays an important role in phloem loading and assimilate uptake, especially in sink tissues, resulting in steep concentration gradient from source to sink (Verma et al 2017). In the initial growth phase (210 DAP), higher CWI expression was found which declined with maturity in stalk.…”

Section: End-point Pcr Analysis Of Genes Associated With Sucrose Accumentioning

confidence: 99%

Expression analysis of genes associated with sucrose accumulation and its effect on source–sink relationship in high sucrose accumulating early maturing sugarcane variety

Verma

Roopendra

Sharma

et al. 2018

Physiol Mol Biol Plants

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Sucrose synthesis/accumulation in sugarcane depends on the source-sink communication wherein source responds to sink demand for photoassimilate supply. Sucrose in stalk (sink) acts as signal, and sends feedback to restrain further synthesis of sucrose by regulating photosynthetic efficiency of leaves (source). Hence sucrose synthesis/accumulation is controlled by many genes and regulatory sequences including 3 invertases (SAI, CWI, NI), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS). SPS and invertase play key role in enhancing sink strength which ultimately promotes greater sucrose accumulation in the sink tissues. In present study, a significant positive correlation was found between sucrose% of source and sink tissues which was greater in the top (R 2 = 0.679) than middle (R 2 = 0.580) and bottom (R 2 = 0.518) internodes, depicting that sucrose accumulation in the stalk bears a direct relation with sucrose translocation efficiency from source. Results indicated an increased sucrose% with maturity, while reducing sugar content decreased with crop growth. qRT-PCR results exhibited an elevated expression of invertase in immature sink tissues depicting increased sink requirement, which declined with maturity. Similarly, increased PEP carboxylase gene expression as observed supported the fact that higher sink demand results in enhanced photosynthetic rate and thus influences the source activity. SPS was found active at initial stage of cane development indicating its role in sucrose synthesis. Thus by studying expression patterns of the different genes both, in source and sink tissues, a better understanding of the sucrose accumulation pathway in sugarcane is possible, which in turn can help in elucidating ways to enhance sucrose concentration in sink.

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“…As a result, the application of GA3 showed a positive impact on some stages of development leading to an increase in cane yield compared to untreated controls. Various research programs revealed that GA3 had an effect on the activity of sucrose phosphosynthase, one of the major enzymes involved in the synthesis of sucrose [7], and promoted the proliferation of sink tissues, leading to an improved assimilation process in sugarcane [8][9][10]; economic efficiency was thereby enhanced. In addition, GA3 increased abiotic stress tolerance in crops like sugarcane that are grown in, for example, salinated environments [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of GA3 and Gly Plant Growth Regulators on Productivity and Sugar Content of Sugarcane

Nguyen¹,

Dang²,

Nguyen³

et al. 2019

Agriculture

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The use of plant growth regulators is one effective solution to improve sugarcane yields and sugar content in several countries. In this study, we examined the role of gibberellin acid (GA3) and glyphosate (Gly) plant growth regulators to determine the appropriate concentration of GA3 and Gly to increase the yield of sugarcane and sugar accumulation, respectively. The statistical results showed that GA3 was sprayed at 150 ppm to increment the actual yield by 19.94%; sucrose accumulation increased by 2.21%. With Gly treatment, although the yield decreased by 3.17%, sucrose accumulation increased by 11.27% compared to control trials. In this study, the combined concentration of 150 ppm of both GA3 and Gly gave the best results, for which sucrose accumulation increased from 2.21% to 10.74% and from 19.94% to 20.97% for actual yield. The results led to increased net income compared to the control. To address concerns about residues of plant growth regulators, residues of GA3 and Gly were evaluated after the sugarcane harvest using the HPLC and UV-vis methods, respectively. The analyzed results showed that their residues were lower than what is permitted in several countries. This showed the applicability of the study, on a large scale, to increase sucrose accumulation, productivity of sugarcane, and profit for farmers.

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Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source–sink perturbed conditions

Cited by 16 publications

References 63 publications

Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage

Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage

Expression analysis of genes associated with sucrose accumulation and its effect on source–sink relationship in high sucrose accumulating early maturing sugarcane variety

Effect of GA3 and Gly Plant Growth Regulators on Productivity and Sugar Content of Sugarcane

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Expression analysis of genes associated with sucrose accumulation in sugarcane under normal and GA3-induced source–sink perturbed conditions

Cited by 16 publications

References 63 publications

Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA3 at grand growth stage

Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA3 at grand growth stage

Expression analysis of genes associated with sucrose accumulation and its effect on source–sink relationship in high sucrose accumulating early maturing sugarcane variety

Effect of GA3 and Gly Plant Growth Regulators on Productivity and Sugar Content of Sugarcane

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Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage

Physio‐biochemical assessment and expression analysis of genes associated with drought tolerance in sugarcane (Saccharum spp. hybrids) exposed to GA₃ at grand growth stage