Role of the shape of various bacteria in their separation by Microthermal Field-Flow Fractionation

Janča, Josef; Halabalová, Věra; Růžička, Jan

doi:10.1016/j.chroma.2010.10.082

Cited by 19 publications

(12 citation statements)

References 17 publications

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“…Finally, it was concluded that the retention of particles larger than 200 nm in diameter is strongly increased by increasing the ionic strength in moderate salinity conditions due to the presence of gravitational and resulting buoyancy forces. These results were supported by the same research group, demonstrating consistent effects by variation of NaCl concentration in the application of bacteria cell separation according to varying cell shapes in microthermal FFF 46…”

Section: Theorysupporting

confidence: 65%

Ion Effects in Field‐Flow Fractionation of Aqueous Colloidal Polystyrene

Lang¹,

Eslahian²,

Maskos

2012

Macro Chemistry & Physics

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We discuss the effect of electrolytes on retention of aqueous colloidal polystyrene particles in asymmetrical flow (AF‐FFF) and thermal field‐flow fractionation (ThFFF). In both FFF subtechniques, interparticle interaction leads to non‐ideal fractionation behavior, which can result in a sample load dependency. Electrostatic repulsion is reduced with increasing electrolyte concentration, resulting in a pronounced increase of retention. At higher salinities, hydrophobic interactions dominate, thus applications of AF‐FFF under physiological conditions are limited. In ThFFF, also the separation mechanism of thermophoresis is affected by ionic shielding and experimental data are in accordance with recent theoretical models of thermophoresis.

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Section: Theorysupporting

confidence: 65%

Ion Effects in Field‐Flow Fractionation of Aqueous Colloidal Polystyrene

Lang¹,

Eslahian²,

Maskos

2012

Macro Chemistry & Physics

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“…The cross‐flow filtration discussed in Qualitative Filtration Principles section is a type of drag‐force‐FFF. Various types of FFF have been used to separate blood components, and separate bacteria from each other, but we have found no reports of its use in separation of bacteria from blood. There are a few reports of separating blood‐dwelling parasites from blood by FFF methods, but these reports take advantage of the much larger size and motility of filamentous parasites compared to RBCs .…”

Section: Other Methodsmentioning

confidence: 99%

Rapid separation of bacteria from blood—review and outlook

Pitt

Alizadeh

Husseini

et al. 2016

Biotechnology Progress

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The high morbidity and mortality rate of bloodstream infections involving antibiotic-resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplification by PCR in the presence of blood components has been problematic. The rapid separation of bacteria from blood would facilitate their genetic identification by PCR or other methods so that the proper antibiotic regimen can quickly be selected for the septic patient. Microfluidic systems that separate bacteria from whole blood have been developed, but these are designed to process only microliter quantities of whole blood or only highly diluted blood. However, symptoms of clinical blood infections can be manifest with bacterial burdens perhaps as low as 10 CFU/mL, and thus milliliter quantities of blood must be processed to collect enough bacteria for reliable genetic analysis. This review considers the advantages and shortcomings of various methods to separate bacteria from blood, with emphasis on techniques that can be done in less than 10 min on milliliter-quantities of whole blood. These techniques include filtration, screening, centrifugation, sedimentation, hydrodynamic focusing, chemical capture on surfaces or beads, field-flow fractionation, and dielectrophoresis. Techniques with the most promise include screening, sedimentation, and magnetic bead capture, as they allow large quantities of blood to be processed quickly. Some microfluidic techniques can be scaled up.

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“…Several FFF-based methods were developed to separate bacteria according to their differences in shape and morphology from environmental and clinical samples, with short analysis times and high sensitivity [120][121][122][123][124] Being FFF characterized by a "soft" separation mechanism, bacteria are separated without modifications of their native properties (e.g., enzymatic activity, cell vitality, quaternary proteins structure) and they can thus be collected and re-cultivated or subjected to further analysis [125,126].…”

Section: Field-flow Fractionationmentioning

confidence: 99%

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

et al. 2012

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Foodborne illnesses caused by pathogenic bacteria represent a widespread and growing problem to public health, and there is an obvious need for rapid detection of food pathogens. Traditional culture-based techniques require tedious sample workup and are time-consuming. It is expected that new and more rapid methods can replace current techniques. To enable large scale screening procedures, new multiplex analytical formats are being developed, and these allow the detection and/or identification of more than one pathogen in a single analytical run, thus cutting assay times and costs. We review here recent advancements in the field of rapid multiplex analytical methods for foodborne pathogenic bacteria. A variety of strategies, such as multiplex polymerase chain reaction assays, microarray-or multichannel-based immunoassays, biosensors, and fingerprint-based approaches (such as mass spectrometry, electronic nose, or vibrational spectroscopic analysis of whole bacterial cells), have been explored. In addition, various technological solutions have been adopted to improve detectability and to eliminate interferences, although in most cases a brief pre-enrichment step is still required. This review also covers the progress, limitations and future challenges of these approaches and emphasizes the advantages of new separative techniques to selectively fractionate bacteria, thus increasing multiplexing capabilities and simplifying sample preparation procedures.

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Role of the shape of various bacteria in their separation by Microthermal Field-Flow Fractionation

Cited by 19 publications

References 17 publications

Ion Effects in Field‐Flow Fractionation of Aqueous Colloidal Polystyrene

Ion Effects in Field‐Flow Fractionation of Aqueous Colloidal Polystyrene

Rapid separation of bacteria from blood—review and outlook

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

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