Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

Roda, Aldo; Mirasoli, Mara; Roda, Barbara; Bonvicini, Francesca; Colliva, Carolina; Reschiglian, Pierluigi

doi:10.1007/s00604-012-0824-3

Cited by 100 publications

(50 citation statements)

References 127 publications

(152 reference statements)

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“…Gold Nps have been intensively studied in the past decade due to their potential in different applications [8,9] such as chemical catalysis, [10] optoelectronic devices, pathogenic bacteria, [11] chemical and bio-sensors, [10,12] catalysts for the growth of aligned one-dimensional (1D) nanostructures [13,14] and biofuel cells. [15] One interesting property of AuNPs is their aggregations when salt is introduced.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles for the bare-eye based and spectrophotometric detection of proteins, polynucleotides and DNA

et al. 2014

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We have explored the potential of using gold nanoparticles (Au-NPs) in optical and bare-eye discrimination of (a) proteins (such as bovine serum albumin and lysozyme), (b) homo-polynucleotides (such as poly-adenylic acid, polycytidylic acid, poly-uridylic acid and poly-inosinic acid), and (c) long chain DNA (from salmon, herring and thym). Such biomacromolecules can be detected and discriminated due to their ability to prevent the formation of blue aggregates from red (non-aggregated) citrate capped Au-NPs on addition of NaCl. The effect of these biomacromolecules on the aggregation was investigated by colorimetry and UV-vis spectrometry. The results show that the two proteins can be differentiated by colorimetry, and also salmon ssDNA and dsDNA. The AuNPs can also discriminate the dsDNAs of salmon and herring. We conclude that the use of Au-NPs represent a viable candidate to future methods of DNA analysis on the basis of visual testing, particularly in the area of food analysis.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticles for the bare-eye based and spectrophotometric detection of proteins, polynucleotides and DNA

et al. 2014

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“…However, the conventional culturebased way for Salmonella detection, which requires enrichment and isolation steps followed by standard plate counts, is timeconsuming and low-efficiency [6]. To date, detection technology for food-borne pathogens has developed from the culture level to the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

et al. 2015

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We describe a Salmonella biosensor that was obtained by electrochemical immobilization of a nanocomposite consisting of reduced graphene oxide (rGO) and carboxymodified multi-walled carbon nanotubes (MWCNTs) directly on the surface of a glassy carbon electrode (GCE). An aminomodified aptamer specific for Salmonella was covalently bound to the rGO-MWCNT composite via amide bonds. The morphology of the rGO-MWCNT nanocomposite was characterized by transmission electron microscopy and scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy were used to monitor all steps during assembly. When exposed to samples containing Salmonella, the anti-Salmonella aptamer on the electrode captures its target. Hence, electron transfer is blocked, and this results in a large increase in impedance. Salmonella can be quantified by this aptasensor, typically operated at a working voltage of 0.2 V (vs. Ag/AgCl), in the range from 75 to 7.5×10 5 cfu⋅mL −1 and detection limit of 25 cfu⋅mL −1 (at an S/N of 3). The method is perceived to have a wide scope in that other bacteria may be detected by analogy to this approach and with very low limits of detection by applying respective analyte-specific aptamers.

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“…They are also a source of foodborne transmission of disease to humans [3][4][5]. Therefore, establishing an effective and fast detection method for these two pathogens is required [6]. Multilocus enzyme electrophoresis and sequence analysis showed that S. pullorum and S. Gallinarum have the same antigen O 1 , O 9 and O 12 , and exhibit high cross-reactivity with each other, so they can be simultaneously detected [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

A sandwich electrochemical immunosensor for Salmonella pullorum and Salmonella gallinarum based on a screen-printed carbon electrode modified with an ionic liquid and electrodeposited gold nanoparticles

2015

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This article describes an electrochemical immunosensor for rapid determination of Salmonella pullorum and Salmonella gallinarum. The first step in the preparation of the immunosensor involves the electrodeposition of gold nanoparticles used for capturing antibody and enhancing signals. In order to generate a benign microenvironment for the antibody, the ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate was used to modify the surface of a screen-printed carbon electrode (SPCE). The single steps of modification were monitored via cyclic voltammetry and electrochemical impedance spectroscopy. Based on these findings, a sandwich immunoassay was worked out for the two Salmonella species by immobilizing the respective unlabeled antibodies on the SPCE. Following exposure to the analytes, secondary antibody (labeled with HRP) is added to form the sandwich. After adding hydrogen peroxide and thionine, the latter is oxidized and its signal measured via CV. A linear response to the Salmonella species is obtained in the 10 4 to 10 9 cfu·mL −1 concentration range, and the detection limits are 3.0×10 3 cfu·mL −1 for both species (at an SNR of 3). This assay is sensitive, highly specific, acceptably accurate and reproducible. Given its low detection limit, it represents a promising tool for the detection of S. pullorum, S. gallinarum, and -conceivably -of other food-borne pathogens by exchanging the antibody.

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Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

Cited by 100 publications

References 127 publications

Gold nanoparticles for the bare-eye based and spectrophotometric detection of proteins, polynucleotides and DNA

Gold nanoparticles for the bare-eye based and spectrophotometric detection of proteins, polynucleotides and DNA

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

A sandwich electrochemical immunosensor for Salmonella pullorum and Salmonella gallinarum based on a screen-printed carbon electrode modified with an ionic liquid and electrodeposited gold nanoparticles

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