Role of Sertoli Cells in Injury-Associated Testicular Germ Cell Apoptosis

Boekelheide, Kim; Fleming, Shawna L.; Johnson, Kamin J.; Patel, Sutchin R.; Schoenfeld, Heidi A.

doi:10.1046/j.1525-1373.2000.22513.x

Cited by 98 publications

(53 citation statements)

References 82 publications

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“…The presence of vacuoles in the cytoplasm of Sertoli cells shows direct damage to these cells. These lesions are early morphological signs of testicular injury and are considered as the main Sertoli cell response to many xenobiotics [2,25,26].…”

Section: Discussionmentioning

confidence: 99%

“…This elaborate process is dependent on hormonal and dynamic interactions between the Sertoli cells and the germ cells [1,2]. Tight junctions between adjacent Sertoli cells create two separate compartments within the seminiferous epithelium: a basal compartment below the tight junction and an adluminal compartment above.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, Sertoli cells provide efficient paracrine signaling mechanisms between these cells as well as physical support to developing germ cells [3]. The intricate regulation and cellular interactions that occur in the testis provide multiple distinct targets by which toxicants can disrupt spermatogenesis [2].…”

Section: Introductionmentioning

confidence: 99%

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The effect of zinc oxide nanoparticles on mouse spermatogenesis

Talebi

Khorsandi

Moridian³

2013

J Assist Reprod Genet

126

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Objective To evaluate the effects of zinc oxide nanoparticles on mouse spermatogenesis. Methods Thirty two adult male NMRI mice were used. Experimental Groups (ZNP-1-ZNP-3) received one of the following treatments daily for 35 days: 5, 50 and 300 mg/kg zinc oxide nanoparticles respectively. Control group received only distilled water. Epididymal sperm parameters, testicular histopathology, morphometric analysis and spermatogenesis assessments were performed for evaluation of the zinc oxide nanoparticles effects on testis. Results Epididymal sperm parameters including sperm number, motility and percentage of abnormality were significantly changed in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.01). Histopathological criteria such as epithelial vacuolization, sloughing of germ and detachment were significantly increased in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.001). 300 mg/kg zinc oxide nanoparticles induced formation of multinucleated giant cells in the germinal epithelium. 50 and 300 mg/kg zinc oxide nanoparticles also caused a significant decrease in seminiferous tubule diameter, seminiferous epithelium height and maturation arrest (p<0.001). Conclusion Zinc oxide nanoparticles act as testicular toxicant and further studies are needed to establish its mechanism of action upon spermatogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of zinc oxide nanoparticles on mouse spermatogenesis

Talebi

Khorsandi

Moridian³

2013

J Assist Reprod Genet

126

View full text Add to dashboard Cite

show abstract

“…This elaborate process is dependent on hormonal or dynamic interactions between the Sertoli cells and the germ cells [1,2] as well as between Leydig cells and germ cells. Tight junctions between adjacent Sertoli cells create two separate compartments within the seminiferous epithelium: a basal compartment below the tight junction and an adluminal compartment above.…”

Section: Introductionmentioning

confidence: 99%

“…Leydig cells synthesis and release testosterone. Testosterone is an essential factor for spermatogenesis process [2]. The intricate regulation and cellular interactions that occur in the testis provide multiple distinct targets by which toxicants can disrupt spermatogenesis [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of beta-carotene on titanium oxide nanoparticles-induced testicular toxicity in mice

Orazizadeh

Khorsandi

Absalan

et al. 2014

J Assist Reprod Genet

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Objective This study evaluated the protective effect of betacarotene (BC) on titanium oxide nanoparticle (TNP) induced spermatogenesis defects in mice. Materials and methods Thirty-two NMRI mice were randomly divided into four groups. BC group received 10 mg/kg of BC for 35 days. TNP group received 300 mg/kg TNP for 35 days. TNP+BC group initially received 10 mg/kg BC for 10 days and was followed by concomitant administration of 300 mg/kg TNP for 35 days. Control group received only normal saline for 35 days. Epididymal sperm parameters, testicular histopathology, spermatogenesis assessments and testosterone assay were performed for evaluation of the TNP and BC effects on testis. Results Serum testosterone levels were markedly decreased in TNP-intoxicated mice. Epididymal sperm parameters including sperm number, motility and percentage of abnormality were significantly changed in TNP-intoxicated mice (p < 0.01). Histopathological criteria such as epithelial vacuolization, sloughing of germ cells and detachment were significantly increased in TNP-intoxicated mice (p<0.001). BC+TNP treatment significantly prevented these changes (p<0.05). BC also significantly elevates testosterone levels in BC+TNP group compared to TNP-treated mice (p<0.01). Discussion and conclusion The results of this study demonstrated that BC improved the spermatogenesis defects in TNPtreated mice. BC had a potent protective effect against the testicular toxicity and might be clinically useful.

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Apoptosis and blood–testis barrier during the first spermatogenic wave in the pubertal rat

Morales

Mohamed

Cavicchia

2007

The Anatomical Record

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This research explores the initial assembly of the blood-testis barrier (BTB) during puberty, when a massive physiological apoptosis in the first spermatogenic wave takes place. Fragments of testis from 14-to 20-dayold rats were studied by conventional transmission electron microscopic techniques. Lanthanum hydroxide was used as an intercellular tracer. Light microscopy was used to confirm apoptotic death when paraffin-embedded sections were studied by TUNEL analysis. When the seminiferous cords reached the zygotene-pachytene spermatocyte level, they exhibited abundant apoptotic figures, whereas the remaining segments showed sporadic apoptosis. We found a BTB not yet assembled in the cords with zygotene-pachytene spermatocytes and abundant apoptosis. The observed apoptosis frequency diminished drastically when BTB was organized, as confirmed by the use of the tracer. Our conclusion is that the massive apoptosis found in the zygotene-pachytene spermatocytes between days 14 and 20 coincides with an open BTB. The absence of BTB could be one of the factors causing massive apoptosis of zygotene-pachytene spermatocytes, at least within the time span analyzed. The zygotene-pachytene spermatocytes are left exposed in an open environment instead of being isolated in the adluminal compartment to which they are destined. Anat

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Role of Sertoli Cells in Injury-Associated Testicular Germ Cell Apoptosis

Cited by 98 publications

References 82 publications

The effect of zinc oxide nanoparticles on mouse spermatogenesis

The effect of zinc oxide nanoparticles on mouse spermatogenesis

Effect of beta-carotene on titanium oxide nanoparticles-induced testicular toxicity in mice

Apoptosis and blood–testis barrier during the first spermatogenic wave in the pubertal rat

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