Apoptosis and blood–testis barrier during the first spermatogenic wave in the pubertal rat

Morales, Alfonsina; Mohamed, Fabián; Cavicchia, Juan C.

doi:10.1002/ar.20417

Cited by 33 publications

(32 citation statements)

References 43 publications

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“…Notably, the majority of apoptotic events in null testes occurred in or around stage IX (supplementary material Fig. S2), a period where spermatogonia stem cells differentiating into spermatocytes cross the tightly regulated blood-testis barrier (BTB) (Bordlein et al, 2011;Cheng and Mruk, 2012;Morales et al, 2007;Mruk and Cheng, 2004;Oishi et al, 2004). Downregulated genes in null testes are expressed normally in spermatogonia, a cell type that in null mice also displayed increases in H3K9me3, indicating that an aberrant repressive chromatin state established in the early stages of null spermatogonia development might impede the expression of genes necessary for differentiation into pre-leptotene spermatocytes and the BTB transition.…”

Section: Discussionmentioning

confidence: 99%

Histone H3.3 regulates dynamic chromatin states during spermatogenesis

et al. 2014

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The histone variant H3.3 is involved in diverse biological processes, including development, transcriptional memory and transcriptional reprogramming, as well as diseases, including most notably malignant brain tumors. Recently, we developed a knockout mouse model for the H3f3b gene, one of two genes encoding H3.3. Here, we show that targeted disruption of H3f3b results in a number of phenotypic abnormalities, including a reduction in H3.3 histone levels, leading to male infertility, as well as abnormal sperm and testes morphology. Additionally, null germ cell populations at specific stages in spermatogenesis, in particular spermatocytes and spermatogonia, exhibited increased rates of apoptosis. Disruption of H3f3b also altered histone post-translational modifications and gene expression in the testes, with the most prominent changes occurring at genes involved in spermatogenesis. Finally, H3f3b null testes also exhibited abnormal germ cell chromatin reorganization and reduced protamine incorporation. Taken together, our studies indicate a major role for H3.3 in spermatogenesis through regulation of chromatin dynamics.

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Section: Discussionmentioning

confidence: 99%

Histone H3.3 regulates dynamic chromatin states during spermatogenesis

et al. 2014

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“…Between the first and sixth week, the first wave of spermatogenesis takes place in the rat gonad [1,2]. Throughout this period, spermatozoa production is limited because of massive cell apoptosis [3], and in this way the proper number of SSCs and SCs [4,5] for sperm production in adult gonads is established [6]. Moreover, at about the 15 th to 18 th postnatal day, the physiological blood-testis barrier is created, the lumen of the seminiferous cords open, and the Sertoli cells undertake their secretory function [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Throughout this period, spermatozoa production is limited because of massive cell apoptosis [3], and in this way the proper number of SSCs and SCs [4,5] for sperm production in adult gonads is established [6]. Moreover, at about the 15 th to 18 th postnatal day, the physiological blood-testis barrier is created, the lumen of the seminiferous cords open, and the Sertoli cells undertake their secretory function [6,7]. The morphology and function of the testis is under the control of hypothalamic-pituitary-gonad axis and locally produced steroid hormones such as testosterone (T) and dihydrotestosterone (DHT), which is much more biologically active than T [8].…”

Section: Introductionmentioning

confidence: 99%

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

Kolasa

Misiakiewicz-Has

Baranowska-Bosiacka

et al. 2015

Folia Histochem Cytobiol.

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Introduction. The hormone-dependent events that occur throughout the first wave of spermatogenesis, such as the establishment of the number of Sertoli cells (SCs) and spermatogonial stem cells (SSCs) within the seminiferous cords and the setting up of the blood-testis barrier, are important for adult male fertility. Any changes in the T/DHT ratio can result in male subfertility or even infertility. In this study we aimed to evaluate effects of paternal exposure to 5-alpha reductase type 2 inhibitor, finasteride on litter size, androgen levels and germ cell apoptosis in male offspring during postnatal development. Material and methods. The subjects of the study were 7, 14, 21/22, 28, and 90-day-old Wistar male rats (F1:Fin) born from females fertilized by finasteride-treated rats. Offspring born from untreated parental animals were used as a control group (F1:Control). Animals and the collected testes were weighed, blood and intratesticular levels of T and DHT were measured by ELISA, and the apoptotic index of testicular cells was evaluated by TUNEL technique. Results. We observed difficulties in obtaining male newborns from female rats fertilized by finasteride-treated male rats. In the F1:Fin rats, changes in the body and testes weights occurred, and a lower number of apoptotic cells was found during postnatal maturation of the seminiferous epithelium. Changes in androgen concentrations during the first spermatogenesis wave and adult life were also evident. Conclusion. Finasteride treatment of male adult rats may not only cause a decrease in the fertility of parental rats, but also could lead to incorrect, androgen-sensitive course of spermatogenesis in their offspring.

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“…The BTB allows for the establishment of a specialised epithelial microenvironment, separated from factors found in the interstitium, which is conducive to meiotic and post-meiotic GC development. In the absence of a functional BTB, spermatogenesis continues only as far as spermatocytes in rodents (Cavicchia & Sacerdote 1991, Gow et al 1999, Morales et al 2007, highlighting the indispensability of the BTB. While intensively studied (for reviews see (Lui et al 2003, Turner 2006, Matter & Balda 2007) the regulation of TJ proteins in the testis is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Tarulli¹,

Meachem²,

Schlatt³

et al. 2008

Reproduction

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This study aimed to assess the effect of gonadotrophin suppression and FSH replacement on testicular tight junction dynamics and blood-testis barrier (BTB) organisation in vivo, utilising the seasonal breeding Djungarian hamster. Confocal immunohistology was used to assess the cellular organisation of tight junction proteins and real-time PCR to quantify tight junction mRNA. The effect of tight junction protein organisation on the BTB permeability was also investigated using a biotin-linked tracer. Tight junction protein (claudin-3, junctional adhesion molecule (JAM)-A and occludin) localisation was present but disorganised after gonadotrophin suppression, while mRNA levels (claudin-11, claudin-3 and occludin) were significantly (two-to threefold) increased. By contrast, both protein localisation and mRNA levels for the adaptor protein zona occludens-1 decreased after gonadotrophin suppression. FSH replacement induced a rapid reorganisation of tight junction protein localisation. The functionality of the BTB (as inferred by biotin tracer permeation) was found to be strongly associated with the organisation and localisation of claudin-11. Surprisingly, JAM-A was also recognised on spermatogonia, suggesting an additional novel role for this protein in trans-epithelial migration of germ cells across the BTB. It is concluded that gonadotrophin regulation of tight junction proteins forming the BTB occurs primarily at the level of protein organisation and not gene transcription in this species, and that immunolocalisation of the organised tight junction protein claudin-11 correlates with BTB functionality.

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Apoptosis and blood–testis barrier during the first spermatogenic wave in the pubertal rat

Cited by 33 publications

References 43 publications

Histone H3.3 regulates dynamic chromatin states during spermatogenesis

Histone H3.3 regulates dynamic chromatin states during spermatogenesis

Androgen levels and apoptosis in the testis during postnatal development of finasteride-treated male rat offspring

Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

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