Role of oxidative stress, infection and inflammation in male infertility

Agarwal, Ashok; Rana, Mohit; Qiu, Emily; AlBunni, Hashem; Bui, Albert; Henkel, Ralf

doi:10.1111/and.13126

Cited by 245 publications

(222 citation statements)

References 152 publications

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“…Otherwise, pathological elevated ROS levels generate a cascade of events leading to plasma membrane lipid peroxidation. The major ROS sources are represented by sperm mitochondria morphologically abnormal spermatozoa and activated leukocytes in the seminal plasma , in response to a wide range of infection/inflammation‐related stimuli in the male genital tract …”

Section: Introductionmentioning

confidence: 99%

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

et al. 2019

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Background Oxidative stress (OS) is one of the most prevalent causes of sperm damage, through the toxic effects of endogenously generated hydrogen peroxide, superoxide anion, and hydroxyl radicals. Peripheral leukocytes represent a feasible model for studying the pathophysiology of OS‐mediated homeostasis, which can be responsible for cell dysfunction and cell injury. Objective To evaluate the redox status in patients with non‐obstructive azoospermia (NOA), establishing the potential role exerted by reactive oxygen species (ROS) in the genesis of testicular secretory injury. Material and methods From May 2018 to March 2019, 39 patients were enrolled in this prospective single‐center cohort study and divided into two groups. Group 1 included 19 patients with NOA, and Group 2 included 20 normozoospermic men, partners of women with infertility tubal factor. All patients underwent serum blood tests. NOA underwent testicular sperm extraction (TeSE). ROS production (in lymphocytes, monocytes, and granulocytes) was assessed by fluorescence‐activated cell sorting (FACS) analysis. Plasma oxidative stress was evaluated by lipid peroxidation markers (MDA) and total antioxidant capacity (TAC) both assessed by fluorometric techniques. Results Mean lymphocyte ROS production resulted 967.0 ± 224.5 vs 728.0 ± 98.0 (NOA vs Controls, P < .001), monocyte ROS resulted 2102.5 ± 517.5 vs 1253 ± 171 (P < .001), and granulocyte ROS were 2366.5 ± 595.4 vs 1751.0 ± 213.0 (P < .001). Significant increases plasma lipid peroxidation markers were found in NOA patients compared with controls (2.7 ± 0.8 vs 0.37 ± 0.2 nmol/mL, P < .001). Significant decreased TAC was evident in NOA compared with controls (13.4 ± 3.9 vs 3.0 ± 0.2 µmol/mL Trolox equivalents, P < .001). No significant differences were found in blood leukocyte subpopulations ROS production, plasma lipid peroxidation, and TAC comparing groups (positive vs negative sperm retrieval, P > .05). Conclusion ROS production can be directly related to disorders of spermatogenesis, leading to severe conditions of male infertility, including azoospermia.

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Section: Introductionmentioning

confidence: 99%

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

et al. 2019

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“…(Agarwal et al, 2018). Interestingly, OS has been associated with sperm alterations and consequently to male infertility.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, OS and inflammation have been suggested to be involved in different pathologies related to male fertility including varicocele, leucocytospermia, sexually transmitted disease, bacterial prostatitis, etc. (Agarwal et al, 2018). This paper aims to investigate the relevance of cPLA 2 and F 2 -IsoP levels in semen and spermatozoa of infertile men affected by leucocytospermia, varicocele or idiopathic infertility; in each group, light and transmission electron microscopies allowed the evaluation of semen quality.…”

Section: Introductionmentioning

confidence: 99%

Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Collodel

Castellini

Iacoponi³

et al. 2019

Andrologia

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Phospholipase A 2 (PLA 2 ) is involved in eicosanoid release, and F 2 -isoprostanes (F 2 -IsoPs), as free radical-generated eicosanoids released by PLA 2 , are indicators of oxidative stress in different human conditions. This study investigated the interplay between cytosolic PLA 2 (cPLA 2 ), F 2 -IsoPs and sperm features in male infertility, when the involvement of oxidative stress has been reported. Semen evaluation was performed following WHO guidelines, sperm ultrastructure was detected by transmission electron microscopy indicating a fertility index, and the percentages of sperm immaturity, apoptosis and necrosis. In sperm cells and seminal plasma, cPLA 2 levels were determined by immunological method, whereas F 2 -IsoPs by mass spectrometry.Sperm concentration, morphology, vitality and fertility index values were significantly lower in infertile groups compared with fertile men. An increase in sperm apoptosis and necrosis (p < .01), apoptosis (p < .01) and immaturity (p < .001) was detected in leucocytospermia, idiopathic infertility and varicocele, respectively. Seminal cPLA 2 showed the highest value in varicocele group (p < .05), whereas seminal F 2 -IsoPs increased in varicocele (p < .001) and leucocytospermia (p < .05) groups. In the whole population, F 2 -IsoP and cPLA 2 levels were positively correlated (p < .05). On the contrary, F 2 -IsoPs and cPLA 2 were not significantly different when investigated in sperm cells. Our data indicate that fatty acid oxidation/metabolism plays a role in different male reproductive pathological conditions. K E Y W O R D Scytosolic PLA 2 , human semen, leucocytospermia, sperm cells, varicocele

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“…Human fertility has declined markedly due to various factors, such as ecological environmental pollution, an increase in work-related stress, unhealthy living habits, and sexually transmitted diseases [1][2][3][4]. The prevalence of infertility ranges from 10 to 15% among couples.…”

Section: Introductionmentioning

confidence: 99%

“…Many factors, such as urogenital infections, endocrine disorders, immunological factors and drug-related damage, affect the male reproductive system and lead to infertility [1,2]. The specific mechanisms underlying the effects of these factors on male infertility may include spermatogenesis, hormone regulation, oxidative stress, and the regulation of spermatogenesis-related genes [16,17].…”

Section: Introductionmentioning

confidence: 99%

Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia

Zhang

et al. 2020

BMC Complement Med Ther

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AbstractsBackground: Qilin pills (QLPs), a classic Traditional Chinese Medicine (TCM) formula for treating male infertility, effectively improve semen quality in clinical trials. This study was designed to evaluate the effects of QLPs on spermatogenesis, reproductive hormones, oxidative stress, and the testis-specific serinekinase-2 (TSSK2) gene in a rat model of oligoasthenospermia. Methods: Forty adult male Sprague-Dawley (SD) rats were randomly divided into four groups. The rat model with oligoasthenospermia was generated by intragastric administration of tripterygium glycosides (TGs) once daily for 4 weeks. Then, two treatment groups were given different doses (1.62 g/kg and 3.24 g/kg) of QLPs once daily for 60 days. Sperm parameters, testicular histology and reproductive hormone measurements, oxidative stress tests, and TSSK2 expression tests were carried out.Results: QLPs effectively improved semen parameters and testicular histology; restored the levels of FSH, LH, PRL, fT, and SHBG; reduced the levels of oxidative stress products (ROS and MDA); increased testicular SOD activity; and restored the expression of spermatogenesis-related gene TSSK2. Conclusion:QLPs have a therapeutic effect on a rat model of oligoasthenospermia, and this effect is manifested as improvement of semen quality and testis histology, gonadal axis stability, decreased oxidative stress, and the regulation of testis-specific spermatogenesis-related gene TSSK2.

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Role of oxidative stress, infection and inflammation in male infertility

Cited by 245 publications

References 152 publications

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia

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Role of oxidative stress, infection and inflammation in male infertility

Cited by 245 publications

References 152 publications

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Cytosolic phospholipase A 2 and F 2 isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia

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Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility