Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia

Zhang, Kaishu; Fu, Ling; An, Qi; Hu, Weihong; Liu, Jianxin; Tang, Xiuming; Ding, Yajie; Lü, Wei; Liu, Xiaowei; Shang, Xue‐Jun; Gu, Yiqun

doi:10.1186/s12906-019-2799-7

Cited by 23 publications

(17 citation statements)

References 41 publications

(44 reference statements)

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“…Using a rat model of oligoasthenospermia, a very recent study by Zhang and colleagues has demonstrated that treatment with QLPs promotes a significant improvement in male reproductive parameters, including spermatogenesis. In this study, testis expression of Tssk2, both at mRNA and protein levels, was decreased in oligoasthenospermia-induced male rats and restored by treatment with QLPs [ 80 ]. Overall, analysis of TSSK deficiencies in infertile men might result in improved therapies or diagnostic tools for male infertility.…”

Section: Clinical Relevance Of Tssk Kinasesmentioning

confidence: 99%

Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†

Salicioni

Gervasi

Sosnik

et al. 2020

Biology of Reproduction

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Male contraception is a very active area of research. Several hormonal agents have entered clinical trials, while potential non-hormonal targets have been brought to light more recently and are at earlier stages of development. The general strategy is to target genes along the molecular pathways of sperm production, maturation, or function, and it is predicted that these novel approaches will hopefully lead to more selective male contraceptive compounds with a decreased side effect burden. Protein kinases are known to play a major role in signaling events associated with sperm differentiation and function. In this review, we focus our analysis on the testis-specific serine kinase (TSSK) protein family. We have previously shown that members of the family of TSSKs are postmeiotically expressed in male germ cells and in mature mammalian sperm. The restricted postmeiotic expression of TSSKs as well as the importance of phosphorylation in signaling processes strongly suggests that TSSKs have an important role in germ cell differentiation and/or sperm function. This prediction has been supported by the reported sterile phenotype of the Tssk6 knockout (KO) mice and of the double Tssk1 and Tssk2 KO mice and by the male subfertile phenotype observed in a Tssk4 KO mouse model. Summary Sentence The established role of TSSKs in spermiogenesis and in male fertility, together with their unique kinase features, strongly supports this family of protein kinases as a very promising drug discovery target for development of a non-hormonal male contraceptive.

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Section: Clinical Relevance Of Tssk Kinasesmentioning

confidence: 99%

Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†

Salicioni

Gervasi

Sosnik

et al. 2020

Biology of Reproduction

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“…RXFP1 is a receptor for relaxin and is highly expressed in the testis and vas deferens (Ivell et al, 2011). A study by Zhang et al reported that the lncRNA NKILA regulates osteogenic differentiation through the RXFP1/AKT pathway and also supports a new finding that the RXFP1 is the upstream molecule of AKT (Zhang, Fu, et al, 2020;Zhang, Wang, et al, 2020;Zhang, Cao, et al, 2020).…”

Section: Discussionmentioning

confidence: 87%

“…

Male infertility is not only a troublesome medical bottleneck but also a serious social problem, which brings hidden danger to the harmony and stability of the family. Oligoasthenospermia is the most common cause of male infertility in the clinic (Zhang, Fu, et al, 2020;Zhang, Wang, et al, 2020;Zhang, Cao, et al, 2020). The diagnosis of oligoasthenospermia is mainly related to sperm concentration, sperm density, morphology and the percentage of sperm forward motility (PR%), etc (Colpi et al, 2018).

…”

mentioning

confidence: 99%

Runjing Decoction alleviated cyclophosphamide‐induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway

Dong

Xia²,

et al. 2021

Andrologia

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Runjing Decoction (RJD) is a prescription of traditional Chinese medicine for the treatment of oligoasthenospermia. However, the molecular mechanism of RJD on oligoasthenospermia still remains unknown. A model of oligoasthenospermia was induced in 30 Sprague Dawley rats by intraperitoneal injection of cyclophosphamide at 35 mg/kg per day for 5 days and treated by intragastric RJD (13.5 g/kg) or L‐carnitine (100 mg/kg) for 14 days. The body weight, testis and epididymis weight, grade A spermatozoa, grade B spermatozoa, the percentage of sperm forward motility (PR%), the sperm activity rate and the sperm density of rats were evaluated before and after RJD treatment. The testis apoptosis was determined by TUNEL staining. The expressions of RXFP1, FoxO1, PI3K, Akt, Bax and Bcl‐2 were determined by qRT‐PCR and Western blot, respectively. After RJD treatment, the grade A spermatozoa, sperm PR%, sperm activity and sperm density were significantly increased relative to those in model rats. Cell apoptosis of testis tissue was reversed by RJD. RJD suppressed cell apoptosis, inhibited the expression of RXFP1, FOXO1, PI3K, AKT and Bax, and promoted the expression levels of Bcl‐2 in testicular tissue of oligoasthenospermia rats. RJD could alleviate sperm quality and testis damage in oligoasthenospermia rats by inhibiting RXFP1/AKT/FOXO1 pathway.

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“…Testosterone and oxidative stress reportedly improved in i n vitro models of Leydig cells treated with different antioxidant compounds [ 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 ]. Similar results were observed across various animal models of induced hypogonadism by using vitamins A and C [ 171 ], zinc [ 172 ], N -acetylcysteine (NAC) [ 173 , 174 ], gallic acid [ 175 ], lycopene [ 176 ], forskolin [ 177 ], resveratrol [ 167 , 178 ], Nigella sativa seed oil [ 179 ], Lycium chinense Mill [ 180 ], Eruca sativa seeds [ 181 ], Moringa oleifera leaves [ 182 ], Schisandra chinensis [ 183 ], Ojayeonjonghwan [ 184 , 185 ], and Qilin pills [ 186 ] ( Supplementary Table S2 ).…”

Section: Management Of Male Hypogonadism Associated With Non-communicable Chronic Diseasesmentioning

confidence: 99%

The Mechanisms and Management of Age-Related Oxidative Stress in Male Hypogonadism Associated with Non-communicable Chronic Disease

Leisegang¹,

Roychoudhury

Sláma

et al. 2021

Antioxidants

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Androgens have diverse functions in muscle physiology, lean body mass, the regulation of adipose tissue, bone density, neurocognitive regulation, and spermatogenesis, the male reproductive and sexual function. Male hypogonadism, characterized by reduced testosterone, is commonly seen in ageing males, and has a complex relationship as a risk factor and a comorbidity in age-related noncommunicable chronic diseases (NCDs), such as obesity, metabolic syndrome, type 2 diabetes, and malignancy. Oxidative stress, as a significant contributor to the ageing process, is a common feature between ageing and NCDs, and the related comorbidities, including hypertension, dyslipidemia, hyperglycemia, hyperinsulinemia, and chronic inflammation. Oxidative stress may also be a mediator of hypogonadism in males. Consequently, the management of oxidative stress may represent a novel therapeutic approach in this context. Therefore, this narrative review aims to discuss the mechanisms of age-related oxidative stress in male hypogonadism associated with NCDs and discusses current and potential approaches for the clinical management of these patients, which may include conventional hormone replacement therapy, nutrition and lifestyle changes, adherence to the optimal body mass index, and dietary antioxidant supplementation and/or phytomedicines.

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Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia

Cited by 23 publications

References 41 publications

Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†

Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†

Runjing Decoction alleviated cyclophosphamide‐induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway

The Mechanisms and Management of Age-Related Oxidative Stress in Male Hypogonadism Associated with Non-communicable Chronic Disease

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