Role of osteopontin in diet-induced brown gallstone formation in rats

Jin, Chang-Guo; Jiang, Fengru; Zhang, Jie; Ma, Junren; Ling, Xiaofeng

doi:10.1097/cm9.0000000000001519

Cited by 3 publications

(2 citation statements)

References 38 publications

(67 reference statements)

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“…Also, when comparing the GII, GIV, and GI, there is an increase in the mRNA OPN expression in their hepatic tissues. The results reported by Jin et al [ 10 ] and Lin et al [ 11 ] agreed with our data and suggested that hepatic mRNA OPN expression was significantly higher in the GSDs group than in GI. Whereas the results of the study by Yang et al [ 44 ] disagreed with our data, which mentioned that protein and mRNA OPN expression was higher in normal tissue than in lithogenic gallbladder tissue.…”

Section: Discussionsupporting

confidence: 93%

“…In 1979, Osteopontin (OPN) was first noticed by Senger, and in 1985 was named by Franzen [ 1 ], OPN is a Latin word osteo means bone and pontin means Bridge, which has a relation with the lusty role of OPN in the body, sometimes OPN mentioned as an early T-lymphocyte activation protein 1 [ 2 ], or secreted phosphoprotein 1 (SPP-1) [ 2 , 3 ], OPN is encoded by SPP1 gene that is a multifunctional soluble cytokine and a matrix-related glycoprotein belongs to the small integrin-binding ligand N-linked glycoprotein [ 4 , 5 ], existing in some fluids of the body such as blood, urine, and milk, and a plurality of tissues [ 6 ] and in many organs like gall bladder, kidney, lungs, breasts, brain, placenta, salivary and sweat glands, pancreas, inner ear, arteries, nerve cell, an immune cell, urinary and reproductive tracts, epithelial cells of the digestive system [ 7 , 8 ], OPN has participated in a different number of physiological processes including the organizing of bone formation/resorption, organizing of the immune system, brain and gut development [ 6 ], OPN has also a pathogenic role in atherosclerosis, tumorigenesis, metastasis, and liver inflammation and injury [ 7 , 1 ], it canchange the hepatic cholesterol metabolism thus participating in the pathogenesis of cholelithiasis in experimental animals [ 9 ], and because of its increased expression in the epithelium of intrahepatic bile stone-loaded ducts, it is closely related to hepatolithiasis [ 10 ]. However, the mechanism and role of OPN in forming cholesterol gallstone (GS) are undetermined [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Abdal,

Naemi

2023

J Adv Vet Anim Res

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Objective: This research was designed to assess the influence of the administration of a lithogenic diet, hydrogen peroxide, and vitamin AD3E on rabbits’ gallstone formation and to envisage the expression of osteopontin (OPN) in their hepatic tissues. Materials and Methods: Twenty-four healthy local mature rabbits of both genders were divided into four equal groups. At the end of the feeding period, samples of blood were taken from all rab¬bits after they had fasted overnight to estimate the serum lipid profile. And some of the hepatic tissue has been preserved at −28°C for molecular analysis and gene expression. Results: The gallstones were formed 100% in the GIII and 50% in the GIV. The mRNA OPN expression showed a significant increase in the GIII when compared with other groups. In Groups III and II, the serum levels of total cholesterol, Triglyceride, L-C, low-density lipoprotein-choles, and VLDL-C were significantly increased when compared with GI, while in GIII, the serum level of high-density lipoprotein-cholesterol was significantly decreased when compared with GI. Conclusion: It was concluded that the expression of the mRNA OPN was increased in the hepatic tissue of gallstone-formed rabbits.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Abdal,

Naemi

2023

J Adv Vet Anim Res

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Macrophages and derived-TNF-α promote lipopolysaccharide-induced upregulation of endogenous β-glucuronidase in the epithelial cells of the bile duct: A possible facilitator of hepatolithiasis formation

Yang

Wang

et al. 2023

Clinics and Research in Hepatology and Gastroenterology

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Effect of Clostridium butyricum on the formation of primary choledocholithiasis based on intestinal microbiome and metabolome analysis

et al. 2023

Journal of Applied Microbiology

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Aims To investigate the function and probable mechanism of Clostridium butyricum in the development of choledocholithiasis. Methods and results The lithogenic diet group and the lithogenic diet + C. butyricum group were used to develop the choledocholithiasis model. During the experiment, Clostridium butyricum suspension was administered to the rats in the lithogenic diet + C. butyricum group. The findings demonstrated that the C. butyricum intervention decreased the Firmicutes/Bacteroidetes ratio in the colon of experimental animals given a lithogenic diet. The relative levels of Desulfovibrio (0.93%) and Streptococcus (0.38%) fell, whereas Lactobacillus (22.36%), Prevotella (14.09%), and bacteria that produce short-chain fatty acids increased. Finally, 68 distinct metabolic products were found based on nontargeted metabonomics, and 42 metabolic pathways associated to the various metabolites were enriched. Conclusions We found that C. butyricum decreased the development of choledocholithiasis. It keeps the equilibrium of the rat's gut microbiome intact and lowers the danger of bacterial infections of the gastrointestinal and biliary systems. It is hypothesized that by controlling lipid metabolism, it may also have an impact on the development of cholelithiasis.

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Role of osteopontin in diet-induced brown gallstone formation in rats

Abstract: Supplemental Digital Content is available in the text

Cited by 3 publications

References 38 publications

Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet

Macrophages and derived-TNF-α promote lipopolysaccharide-induced upregulation of endogenous β-glucuronidase in the epithelial cells of the bile duct: A possible facilitator of hepatolithiasis formation

Effect of Clostridium butyricum on the formation of primary choledocholithiasis based on intestinal microbiome and metabolome analysis

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