Role of Nitric Oxide in Urinary Bladder Function: Effect of &lt;i&gt;L&lt;/i&gt;-Arginine

Whitbeck, Catherine; Chichester, Paul; Sokol, Rebekah; Levin, Robert M.

doi:10.1159/000096931

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…Regarding the effect of L-arginine treatment, our study did not show a great reduction of the level of dysfunction at 2-wk obstruction, which was different from our previous study (38). We believe that this relates to the severity of the obstruction.…”

Section: Discussioncontrasting

confidence: 99%

“…Our earlier publications studied the acute effects of L-NAME and arginine using 1-and 2-wk obstructions (8,38). These studies demonstrated clearly that both treatments had significant effects on bladder function via both changes in blood flow and changes in the generation of RNS free radicals.…”

Section: Discussionmentioning

confidence: 95%

“…Similarly, other studies have shown that exogenously administered L-arginine decreases oxidative stress in the liver and brain (9) and that an increase in NO formation has a beneficial effect on the progression of cellular and molecular parameters of tubulointerstitial fibrosis caused by obstruction of the ureter (27). With regards to bladder function, our previous study demonstrated that feeding rabbits a diet high in L-arginine was beneficial for both control rabbits and those with 2 wk of severe partial bladder outlet obstruction (PBOO) (38).…”

mentioning

confidence: 99%

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Effects ofl-arginine andl-NAME on chronic partial bladder outlet obstruction in rabbit

Lin

Levin²,

Chichester³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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(NO) is synthesized from L-arginine by nitric oxide synthase (NOS). NOS can be inhibited by N G -nitro-L-arginine methyl ester (L-NAME) and stimulated by supplementing the diet with L-arginine. The aim of this study was to investigate the influence of NOS activity on the response of rabbits to chronic partial bladder outlet obstruction (PBOO). Surgical PBOOs (2 and 8 wk) were performed on male New Zealand White rabbits. Before obstruction, one-third of the animals were premedicated for 7 days with L-NAME and another third with L-arginine. The results are summarized as follows. First, bladder weight after 8-wk PBOO was significantly lower in animals treated with L-arginine compared with both untreated and rabbits treated with L-NAME. Second, contractile function decreased progressively with PBOO duration. However, after 8 wk of PBOO, the L-arginine group had significantly greater contractile function compared with the notreatment group, and the L-NAME group had significantly lower contractile function compared with the no-treatment group. Third, at 8 wk following PBOO, the level of protein oxidation and nitration was lowest for the L-arginine group and highest in the L-NAME group. These studies clearly demonstrated that increasing blood flow by stimulating NOS significantly protected the bladder from PBOO dysfunctions, whereas inhibiting blood flow by L-NAME enhanced the dysfunctions mediated by PBOO. contractility; angiogenesis; nitric oxide NITRIC OXIDE (NO), a neurotransmitter responsible for relaxation activity in the lower urinary tract and corpus cavernosum, is synthesized from L-arginine in a reaction catalyzed by NO synthase (NOS) (6). NO is also known to be a powerful and ubiquitous regulator of vascular tone, and thus in several systems is shown to regulate blood flow. In cardiac studies, impaired release of NO may be an important factor in the development of ischemia-reperfusion (I/R) injury. Conversely, it has been demonstrated that administration of NO donors or L-arginine reduces infarct size and improves the postischemic recovery of cardiac performance and endothelial function in animal models of myocardial ischemia and reperfusion (28,36,37). Similarly, other studies have shown that exogenously administered L-arginine decreases oxidative stress in the liver and brain (9) and that an increase in NO formation has a beneficial effect on the progression of cellular and molecular parameters of tubulointerstitial fibrosis caused by obstruction of the ureter (27). With regards to bladder function, our previous study demonstrated that feeding rabbits a diet high in L-arginine was beneficial for both control rabbits and those with 2 wk of severe partial bladder outlet obstruction (PBOO) (38).Contradicting the protective effects, NO is reported to generate free radicals in I/R injury, which has been shown in other studies to be one of the primary causes of progression of bladder dysfunction (3). It has been demonstrated that NO-related damage due to nitrotyrosine was elevated with PBOO (23). Pretreatment wi...

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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Effects ofl-arginine andl-NAME on chronic partial bladder outlet obstruction in rabbit

Lin

Levin²,

Chichester³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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“…This trend was reversed by L-arginine administration. Similar to our study, rabbits with bladder outlet obstruction undergoing L-arginine stimulation also showed significantly better compliance than the control obstructed rabbits [9]. Prior results suggest that NOS is densely localized in the parasympathetic postganglionic nerve fibers of the lower urinary tract.…”

Section: Discussionsupporting

confidence: 88%

“…L-NAME resulted in more bladder dysfunction 1 day after partial bladder outlet obstruction (PBOO) but protected the bladder from nitric oxide generated free radical damage at later time periods by inhibiting the generation of nitrotyrosine. Another study showed that feeding rabbits a high L-arginine diet can reduce bladder dysfunction following PBOO [9]. These results showed that NO played an important role in regulating bladder function during bladder ischemia and reperfusion.…”

Section: Introductionmentioning

confidence: 90%

The immediate effect of nitric oxide on the rabbit bladder after ovariectomy

Juan¹,

Mannikarottu²,

Schuler³

et al. 2008

Nitric Oxide

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Ovariectomy resulted in decreased blood flow and hypoxia to the bladder mucosa and smooth muscle. Nitric oxide (NO) played an important role in regulating bladder function during bladder ischemia and reperfusion. This study was designed to evaluate the role of NO on bladder function in the first few days after ovariectomy. Female rabbits were separated into three groups, one which received no medication, pre-medicated with N(omega) -nitro-L-arginine methyl ester (L-NAME) and the third treated with L-arginine. Non-ovariectomized controls and at 1 and 3 days post-ovariectomy, animals from each group were euthanized. Cystometry and in vitro isometric contractile responses were recorded and the oxidative stress markers, nitrotyrosine and protein carbonylation were determined. L-NAME treatment did not significantly alter bladder function after ovariectomy. L-arginine fed, ovariectomized rabbits had lower intravesical pressure and better contractile responses to all forms of stimulation than the ovariectomized rabbits with or without L-NAME. Furthermore, the ovariectomized ones with or without L-NAME had higher oxidative stress markers than L-arginine fed rabbits. This study clearly demonstrates that feeding rabbits with L-arginine can protect the bladder from oxidative free radical damage following short term ovariectomy.

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