The immediate effect of nitric oxide on the rabbit bladder after ovariectomy

Abstract

Ovariectomy resulted in decreased blood flow and hypoxia to the bladder mucosa and smooth muscle. Nitric oxide (NO) played an important role in regulating bladder function during bladder ischemia and reperfusion. This study was designed to evaluate the role of NO on bladder function in the first few days after ovariectomy. Female rabbits were separated into three groups, one which received no medication, pre-medicated with N(omega) -nitro-L-arginine methyl ester (L-NAME) and the third treated with L-arginine. Non-ovariectomized controls and at 1 and 3 days post-ovariectomy, animals from each group were euthanized. Cystometry and in vitro isometric contractile responses were recorded and the oxidative stress markers, nitrotyrosine and protein carbonylation were determined. L-NAME treatment did not significantly alter bladder function after ovariectomy. L-arginine fed, ovariectomized rabbits had lower intravesical pressure and better contractile responses to all forms of stimulation than the ovariectomized rabbits with or without L-NAME. Furthermore, the ovariectomized ones with or without L-NAME had higher oxidative stress markers than L-arginine fed rabbits. This study clearly demonstrates that feeding rabbits with L-arginine can protect the bladder from oxidative free radical damage following short term ovariectomy.

Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum

“…An initial resting tension of 2 g was applied for 30 min, and contractile responses were recorded isometrically using a force-displacement transducer. The maximal response was determined sequentially for electrical field stimulation (EFS; 2, 8, and 32 Hz), carbachol (20 M), and KCl (120 mM) (32).…”

Therapeutic Effect of Platelet-Rich Plasma Improves Bladder Overactivity in the Pathogenesis of Ketamine-Induced Ulcerative Cystitis in a Rat Model

“…Based on previous described [ 60 , 89 , 90 , 91 ], the bladders were recorded for weight and cut into three longitudinal strips measuring 0.5 × 1.5 cm 2 from the bladder dome to the trigone area. Contractile responses were performed using a force-displacement transducer.…”

Comparative Evaluation of Antioxidant Reactivity between Ovariectomized and Control Urinary Bladder Tissue Using Ferric Reducing Ability of Plasma and Cupric Ion Reducing Antioxidant Capacity Assays

“…11 Previous studies in rabbits showed that ovariectomy causes decreased serum estrogen, decreased urothelial oxygenation and blood flow, ischemia, oxidative stress, hypoxia, decreased contractile responses, increased collagen, decreased free fatty acids and phospholipids and urothelial apoptosis. 11,12 In rat models, ovariectomy caused oxidative stress and unstable vesicular and urethral pressures. 13 Male rabbit models, which showed similar ischemic conditions displayed a clear increase in the oxidation of all subcellular components of the bladder tissue during obstruction.…”