Role of Myeloperoxidase, Paraoxonase, and Nitric Oxide System in the Blood and Pericardial Fluid of Patients with Ischemic Heart Disease after Direct Myocardial Revascularization

Eroğlu

et al. 2020

Preprint

Objectives:The purpose ofthis randomized prospective study was to investigate the efficacy and clinical results of del Nido solution (DNS) use in coronary artery bypass grafting (CABG) surgery compared to intermittent warm blood cardioplegia (IWBC). Methods:Thirty-nine adult patients who underwent primary isolated CABG surgery with cardiopulmonary bypass (CPB) between June 2018 and February 2019 were included in the study. DNS was used in 17 patients and IWBC in 22. The clinical and laboratory results were then compared. Results:Aortic cross-clamp time and CPB time were both lower with DNS. However, we observed no statistically significant difference in clinical results between the two methods, including postoperative myocardial enzymes. Conclusion:Single-dose DNS reduced CPB and aortic cross-clamp times, and provided similar short-term clinical and laboratory outcomes to a conventional multi-dose BC strategy in isolated CABG surgery.

Section: Discussioncontrasting

confidence: 64%

Comparison of Del Nido and Intermittent Warm Blood Cardioplegia in Adult Coronary Artery Bypass Surgery: Randomized, Prospective, Single Center Trial

Eroğlu

et al. 2020

Preprint

“…Relaxation of vascular smooth muscle elements under the action of nitroglycerin is explained by the release of NO during the metabolism of this drug. In patients with myocardial infarction, an inverse correlation was found between the level of nitrites/nitrates and risk factors for the development of left ventricular failure and the severe clinical course of myocardial infarction [10].…”

Section: Introductionmentioning

confidence: 94%

Dynamics of the Nitrergic System in Experimental Hypercholesterolemia

Ph.D.

2023

IJSCM

Hypercholesterolemia and atherosclerosis are still the main cause of mortality in Central Asia. Study of the molecular mechanisms of endothelial dysfunction with changes in the nitroergic system in experimental hypercholesterolemia. The experiments were carried out on 28 Chinchilla rabbits with an average weight of 2.5-3.0 kg. The action of drugs was studied in dynamics: the initial 3-month condition and after one month of drug administration. The results obtained were compared with those of the control and intact groups. The activity of the enzyme nitrate reductase in the blood serum on the 30th day of the introduction of exogenous cholesterol increases only 1.15 times, then on the 60th and 90th days of the introduction - 1.3 and 1.76 times. In the dynamics of hypercholesterolemia and atherosclerosis in the blood serum, there are noticeable disturbances in the NO-ergic system.

Ulyanovsk Medico-Biological Journal

Characteristics of Redox Status in Male and Female Rats With Experimental Diabetes Mellitus, Guerin's Carcinoma and Associated Pathology

Goroshinskaya

Frantsiyants

Kaplieva

et al. 2021

Taking into account cancer and diabetes comorbidity, the role of free radicals in these conditions and the dependence of pathological processes on the gender of animals, the aim of the study was to examine lipid peroxidation (LPO) intensity and the activity of the key antioxidant enzyme superoxide dismutase (SOD) in the heart, kidneys, liver, and in tumors of male and female rats with diabetes mellitus (DM), Guerin's carcinoma (GC) or both diseases. Materials and Methods. The study included 80 white nonlinear male and female rats (180–220 g). The animals were divided into 4 groups, each group included 10 animals (either male or female). At the time of GC inoculation, the blood glucose level in animals with alloxan diabetes was 25.4±1.2 mmol/L. The authors used conventional spectrophotometric methods to examine the content of malondialdehyde (MDA), diene conjugates (DC), and SOD activity. Statistical analysis was performed using Statistical analysis software Statistica 10.0. Results. The most pronounced changes in the studied parameters were found in the heart of female rats with isolated GC and associated diabetes mellitus: more than a threefold increase in MDA, a significant increase in DC against the background of an increase in SOD activity (by 5.5–6.3 times in comparison to intact animals). The amount of MDA in GC tissue depended on the tumor size: the maximum increase in both parameters was observed in male rats with GC growth and associated DM. Conclusion. The changes in the content of LPO products and SOD activity in the heart, kidneys, and liver of rats with diabetes mellitus and tumor growth depend on the type of the examined tissue and the gender of the animals. Disorders of the redox status found in the heart tissue can make a significant contribution to cardiopathology, which is often observed in diabetes mellitus and malignant tumor growth. Key words: diabetes mellitus, Guerin's carcinoma, rats, combined pathology, heart, kidneys, liver, malondialdehyde, diene conjugates, superoxide dismutase. Учитывая коморбидность рака и диабета, роль свободнорадикальных процессов при этих состояниях и зависимость течения патологических процессов от пола животных, целью исследования явилось изучение интенсивности перекисного окисления липидов (ПОЛ) и активности ключевого антиоксидантного фермента супероксиддисмутазы (СОД) в сердце, почках, печени, а также в опухоли крыс разного пола при сахарном диабете (СД), карциноме Герена (КГ) и при их сочетании. Материалы и методы. В исследование были включены 80 белых нелинейных крыс обоего пола массой 180–220 г, разделенных на 4 группы по 10 животных каждого пола. На момент перевивки КГ уровень глюкозы в крови животных с аллоксановым СД составил 25,4±1,2 ммоль/л. Содержание малонового диальдегида (МДА), диеновых конъюгатов (ДК) и активность СОД исследованы общепринятыми спектрофотометрическими методами. Статистический анализ проведен с использованием программы Statistica 10.0. Результаты. Наиболее выраженные изменения изученных показателей выявлены в сердце самок при изолированной КГ и КГ, растущей на фоне СД: более чем трехкратное увеличение МДА, значимый прирост ДК на фоне повышения активности СОД в 5,5–6,3 раза относительно интактных животных. В ткани КГ прослеживалась зависимость степени выраженности увеличения содержания МДА от размеров опухоли: максимальное увеличение обоих показателей наблюдалось у самцов при росте КГ на фоне СД. Выводы. Направленность изменения содержания продуктов ПОЛ и активности СОД в сердце, почках и печени крыс при СД и опухолевом росте зависит от типа исследованной ткани и пола животных. Обнаруженные в ткани сердца нарушения редокс-статуса могут вносить значимый вклад в развитие кардиопатологии, часто наблюдаемой при СД и злокачественном росте. Ключевые слова: сахарный диабет, карцинома Герена, крысы, сочетанная патология, сердце, почки, печень, малоновый диальдегид, диеновые конъюгаты, супероксиддисмутаза.