Role of Lytic Enzymes Secreted by Lysobacter capsici YS1215 in the Control of Root-Knot Nematode of Tomato Plants

Lee, Yong Seong; Nguyen, Xuan Hoa; Naing, Kyaw Wai; Park, Yun Suk; Kim, Kil Yong

doi:10.1007/s12088-014-0499-z

Cited by 31 publications

(15 citation statements)

References 26 publications

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“…Therefore, this result shows that the cuticle of the eggs could be degraded by chitinase secreted by microorganisms. [50] Studies concerning the inhibition of egg hatching and degradation of nematodes eggshells show that the application of chitinase in the biological control of plant pathogenic diseases is possible.…”

Section: Inhibition Of Egg Hatching and Degradation Of Nematodes Eggsmentioning

confidence: 99%

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Lee

Kim

2015

Biotechnology & Biotechnological Equipment

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In the present research, statistical PlackettÀBurman design (PBD) and central composite design (CCD) were used to optimize the medium's components in order to enhance the chitinase activity of Pseudomonas fluorescens strain HN1205. Among the seven nutritional elements that were studied, crab shell powder, CaCl 2 ¢2H 2 O and yeast extract were proved using PBD to have significant effect on chitinase activity. An optimal medium was obtained by applying a three-factor central composite design, which consisted of crab shell powder (1.0 g/L), CaCl 2 (0.5 g/L) and yeast extract (0.5 g/L), with the highest chitinase activity of 1.03 U/mL. This value was 2.87-fold higher than the activity obtained in the lowest productive medium in the design matrix. The chitinase produced by the HN1205 strain was partially purified with 80% ammonium sulphate and anion-exchange chromatography and assessed for inhibition of hatching of nematode eggs. The partially purified chitinase significantly reduced the hatch of Meloidogyne incognita eggs (8.1%), as compared to the effect of 10 mmol/L Tris-HCl buffer (pH 8.0) (49.4%) and boiled chitinase (54.7%). This study demonstrated the role of chitinolytic enzymes produced by P. fluorescens strain HN1205. The obtained optimal activity proved that the enzymes could be potential biological control agents.

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Section: Inhibition Of Egg Hatching and Degradation Of Nematodes Eggsmentioning

confidence: 99%

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Lee

Kim

2015

Biotechnology & Biotechnological Equipment

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“…The direct promotion of plant growth by PGPR provides the plant with phytohormones [24][25][26], which facilitate the uptake of nutrients from the soils [24,25,27,28] and help establish a symbiosis with rhizobia or mycorrhiza [29]. The indirect promotion of plant growth occurs when PGPR lessen or prevent the deleterious effects of pathogenic organisms [30][31][32][33][34][35][36][37], nematodes, and insects [38][39][40] by producing antagonistic substances, such as hydrolytic enzymes, including chitinases, glucanases, proteases, and lipases. The Bacillus species is one of the most important microorganisms in PGPR and is isolated from rhizosphere soil [25,26,34,37,39,41].…”

Section: Introductionmentioning

confidence: 99%

“…The Bacillus species is one of the most important microorganisms in PGPR and is isolated from rhizosphere soil [25,26,34,37,39,41]. The benefits of Bacillus licheniformis strains include the production of plant hormones, such as auxin and gibberellin, nitrogen fixation, and the solubilization of inorganic phosphate, which lead to increased Plant growth-promoting rhizobacteria (PGPR) are beneficial native soil bacteria that are an environmentally sound way of increasing plant yields by facilitating plant growth through either a direct or an indirect mechanism [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. The direct promotion of plant growth by PGPR provides the plant with phytohormones [24][25][26], which facilitate the uptake of nutrients from the soils [24,25,27,28] and help establish a symbiosis with rhizobia or mycorrhiza [29].…”

Section: Introductionmentioning

confidence: 99%

The Control of Fusarium Root Rot and Development of Coastal Pine (Pinus thunbergii Parl.) Seedlings in a Container Nursery by Use of Bacillus licheniformis MH48

Won

Choub

Kwon

et al. 2018

Forests

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This study investigated the control of Fusarium root rot and development of coastal pine (Pinus thunbergii) seedlings in a container nursery by using Bacillus licheniformis MH48. High-quality seedlings without infectious diseases cause vigorous growth. Fusarium root rot caused by Fusarium oxysporum is responsible for serious damage to coastal pine seedlings in nurseries. B. licheniformis MH48 produced enzymes that degraded the fungal cell walls, such as chitinase and β-1,3-glucanase. These lytic enzymes exhibited destructive activity toward F. oxysporum hyphae, which were found to play key roles in the suppression of root rot. In addition, B. licheniformis MH48 increased the nitrogen and phosphorus in soils via fixed atmospheric nitrogen and solubilized inorganic phosphate. B. licheniformis MH48 produced the phytohormone auxin, which stimulated seedling root development, resulting in increased nutrient uptake in seedlings. Both the bacterial inoculation and the chemical fertilizer treatments significantly increased seedling growth and biomass, and the bacterial inoculation had a greater effect on seedling development. Based on the results from this study, B. licheniformis MH48 showed potential as a biological agent against Fusarium root rot and as a promoter of growth and development of Pinus thunbergii seedlings.

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“…Purified chitinase from Lysobacter capsici effectively disrupted the egg shell of Meloidogyne spp. and significantly reduced hatching (Lee et al 2015). The present study aims to (a) isolate and identify some lytic bacterial strains from soil adhering to tomato plant roots, (b) evaluate their production of chitinase, chitosanase and protease, and (c) investigate the nematicidal potential of the isolated strains against Meloidogyne incognita and egg hatchability under in vitro conditions.…”

Section: Introductionmentioning

confidence: 93%

“…The basic rationale behind their use is that, the biochemical composition of nematode structure includes collagens and lipids during the mobile stages, as well as chitin, protein, and lipids in the sedentary stages for tylenchoid nematodes such as Meloidogyne spp. The egg shell of root-knot nematode is composed of three main layers: (a) the outer vitelline layer that support the structural uniformity of eggs (Wharton 1980); (b) the middle chitinous layer of the egg shell which contains chitin fibrils embedded in a protein matrix (Lee et al 2015), and (c) the inner lipid layer which maintain the impermeability of the shell (Bird and McClure 1976). The chitinous layer provides protection for the lipid layer and structural strength.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita

et al. 2019

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Background: Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their nematicidal activity against Meloidogyne incognita J 2 mortality and egg hatching in in vitro test. Results: Lytic Rhizobacteria were isolated from soil samples adhering to tomato plant roots from different localities at Giza governorate, Egypt. Six bacterial isolates, exhibited high efficacy against root-knot nematodes, were identified based on the analysis of the 16S rRNA gene sequence as Pseudomonas aeruginosa, Paenibacillus polymyxa, Lysinibacillus sphaericus, Bacillus cereus, Bacillus subtilis, and Achromobacter xylosoxidans. These strains showed high production of chitinase, chitosanase, and protease using colloidal chitin and soluble chitosan as carbon sources. Two strains, Paenibacillus polymyxa and Bacillus subtilis, produced the highest levels of chitinase in the media. Except for Pseudomonas aeruginosa, all the bacterial strains produced high levels of chitosanase. Conclusion: The results revealed that high amounts of protease were, however, secreted by Pseudomonas aeruginosa as compared to the other strains. In in vitro tests, all the bacterial culture filtrates potentially displayed nematicidal effect in M. incognita egg hatching and an obvious increase in J 2 mortality as compared to control. Paenibacillus polymyxa caused 100% juvenile mortality followed by Bacillus subtilis 97.25%, Bacillus cereus 94%, Achromobacter xylosoxidans 93%, Lysinibacillus sphaericus 92%, and Pseudomonas aeruginosa 84.29% after 48 h of exposure, as compared to control.

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Role of Lytic Enzymes Secreted by Lysobacter capsici YS1215 in the Control of Root-Knot Nematode of Tomato Plants

Cited by 31 publications

References 26 publications

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

The Control of Fusarium Root Rot and Development of Coastal Pine (Pinus thunbergii Parl.) Seedlings in a Container Nursery by Use of Bacillus licheniformis MH48

In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita

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