Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

Abstract

A system for growing in liquid medium whole plants of Valeriana glechomifolia, endemic to southern Brazil and capable of accumulating bioactive valepotriates, is described. Murashige and Skoog (MS) and Gamborg B5 (B5) media (1.0·, 0.3· and 0.1· strength) without phytohormones were evaluated after four weeks of culture in relation to growth and valepotriate yield. Plants grown in 1.0· MS displayed greatest growth and valepotriate yields and the study of the light condition showed that plants grown under light and dark had similar weight increase and maximum valepotriate yield, 27.2 mg/g DW and 25.0 mg/g DW, respectively. Valtrate was the most abundant valepotriate, followed by acevaltrate and didrovaltrate.

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

“…In both media higher dry weight values were obtained by roots grown in half strength MS and MSV media, 2.22 to 1.62 g l -1 and 2.658 to 2.01 g l -1 , respectively. These results are in contrast with the results of Russowski et al (2006) that studied the growth of whole plant in liquid culture and Chen et al (2003) for taxol production in cell cultures of Taxus yunnanensis. High concentrations of inorganic nutrients in the full strength MS and MSV media may be the cause of these results.…”

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

“…The overall results confirm, for long-term cultures in solid media, the direct relationship between growth and valepotriate production previously established for shorter term whole-plant, cell and root cultures of V. glechomifolia grown in liquid media Russowski et al 2006). Moreover, the media modifications in M Δ were able to significantly enhance growth and production of valepotriates, maintaining physiological viability and providing an adequate formulation for long-term stock cultures.…”

“…Further studies analyzed the growth kinetics and the influence of the auxins indole-3-acetic acid, indole-3-butyric acid, and α-naphthaleneacetic acid in micropropagated V. glechomifolia and survival under ex vitro conditions after plant acclimatization (De Carvalho et al 2004). Production of valepotriates has also been studied in V. glechomifolia tissue cultures, such as callus, cell suspension, and root cultures ; the roles of light and medium composition on growth and valepotriate contents in whole plants cultured in liquid medium were also investigated (Russowski et al 2006).…”

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

“…Several authors have reported the value of liquid media for shoot multiplication (Russowski et al 2006;Pati et al 2011;Cavallaro et al 2014). However, S. alpina shoot tip explants placed in liquid culture, either agitated or stationary, did not derive shoots, and tissue necrosis was observed, probably caused by the continuous contact between the plant tissue and the liquid medium.…”