Role of IL-13 in a model of Strongyloides venezuelensis infection in rats

Ferreira, Caroline M.; Pereira, Ana Terezinha M.; Coelho, Fernanda; Poole, Stephen; Teixeira, Mauro Martins; Negrão-Corrêa, Déborah

doi:10.1016/j.micinf.2010.01.012

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…In vivo animal experiments have shown the critical role of IL-13 in the development of asthma. 15 16 In vitro , IL-13 showed a significant effect on various cellular processes within the airway and correlated with the clinical aspects of airway diseases. 17 Specifically, IL-13 is found to induce apoptosis and fibrosis in lung fibroblasts 18 and cooperates with alveolar macrophages and dendritic cells to promote inflammation and chemokine release.…”

Section: Introductionmentioning

confidence: 98%

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Shi

Cao

et al. 2018

Allergy Asthma Immunol Res

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PurposeMolecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism.MethodsThe effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated. The effects of miR-155-5p on the proliferation and migration of interleukin (IL)-13-induced BSMCs was determined in vitro using BSMCs transfected with miR-155 mimic/inhibitor and induced by a high concentration of IL-13. The quantitative real-time polymerase chain reaction (qRTPCR) was employed for determining the expression of miR-155-5p and TAB2. Western blotting was applied to analyze the expression of TAB2 at the protein level. Cell proliferation and migration were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays, respectively.ResultsThe proliferation and migration of BSMCs were dose-dependently increased with IL-13 treatment. Contrariwise, IL-13 dose-dependently inhibited the expression of miR-155-5p in BSMCs. Mechanistic studies showed that inhibition of miR-155-5p further promoted the stimulatory effects of IL-13, whereas overexpression of miR-155 significantly inhibited these effects. In silico studies and luciferase reporter assays indicated that TAB2 was a negatively regulated miR-155-5p target.ConclusionsThese results suggested that miR-155-5p-inhibit the IL-13-induced proliferation and migration of BSMCs by targeting TAB2 and that the IL-13/miR-155/TAB2 pathway could serve as a therapeutic target for pulmonary diseases, especially asthma.

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Section: Introductionmentioning

confidence: 98%

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Shi

Cao

et al. 2018

Allergy Asthma Immunol Res

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“…Strongyloides venezuelensis tem sido utilizado como modelo experimental para estudo da estrongiloidíase humana pela capacidade natural de infectar roedores (MARRA et al, 2011;NAKANISHI, 2014) e pela semelhança do ciclo biológico com S. stercoralis. As larvas de S. venezuelensis penetram por via cutânea, migram para os pulmões, são deglutidas e se instalam na mucosa do intestino delgado (SATO; TOMA, 1990;FERREIRA et al, 2010). Por possuir essas características, a utilização de larvas filarioides de S. venezuelensis constitui uma alternativa para obtenção de antígenos heterólogos utilizados no diagnóstico sorológico da estrongiloidíase humana.…”

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Avaliação parasitológica e sorológica de coinfecção por Syphacia muris e Strongyloides venezuelensis

Sousa¹

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Agradeço, primeiramente, a Deus por me proteger e sempre estar ao meu lado, em todos os momentos da minha vida.Aos meus pais Mônica e José Carlos que não mediram esforços para que eu chegasse até esta etapa de minha vida. Obrigado por ter me fornecido está oportunidade. Gostaria de agradecer especialmente minha mãe mesmo à distância, seu cuidado е dedicação foi que deram, em alguns momentos, а esperança para seguir. Aos meus irmãos Wanderlei, Diego e Karla por serem acima de tudo, meus amigos. A minha família principalmente meus avós paternos José Eduardo e Sebastiana e aos maternos Otacílio e Ivete, por serem exemplos de pessoas humildes e honestas. Aos meus sobrinhos Camila e Vinícius. A todos os meus tios (as) e primos (as). Devo tudo o que sou hoje, a minha família. Muito obrigado a todos. À Profa. Dra. Julia Maria Costa Cruz, pela orientação, oportunidade e dedicação, mostrando-se sempre disposta a resolver os problemas que surgiram neste período de dois anos. Quero expressar o meu reconhecimento e admiração pela sua competência profissional, por ser uma profissional extremamente qualificada e pela forma humana que conduziu minha orientação. Muito Obrigado por tudo. A toda equipe do laboratório Daniela, Gabriela, Lucas, Renata, Camila, Nágilla e Henrique que se tornaram grandes companheiros e amigos. Agradeço em especial meu colega Edson por todo apoio e cumplicidade e que durante esse período acabou se transformando em um grande amigo. As minhas colegas de mestrado Elivaine, Layane, Leilaine, Melina, Pâmela e Letícia. Agradeço a todas pelo convívio e troca de experiências. Gostaria de agradecer especialmente aos colegas Murilo e Eliezer que se tornaram grandes amigos no decorrer destes dois anos. Aos funcionários do laboratório de Parasitologia Juliana, Scheila e Elaine. Agradeço em especialmente a Maria do Rosário e ao Marcelo pela paciência e disponibilidade nunca negada.As pessoas que ao longo do tempo, desde a infância viram meu crescimento enquanto pessoa, enquanto amigo, enquanto estudante e em busca de novos sonhos e horizontes,

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The role of IL-33/ST2, IL-4, and eosinophils on the airway hyperresponsiveness induced by Strongyloides venezuelensis in BALB/c mice

Araújo

Pereira

et al. 2016

Parasitol Res

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Strongyloidiasis is a neglected chronic nematode infection, in which the control of autoinfection rate and severity of disease is dependent on type 2 immune responses. Strongyloides also causes Th2 responses in the lung of infected animals and changes in airway function, including airway hyperresponsiveness (AHR). Mechanisms of AHR during Strongyloides venezuelensis infection are not entirely known, and we investigate here the role of IL-4, eosinophils, and IL-33/ST2. AHR was evaluated in infected mice by determining changes in lung function after increasing doses of methacholine. Balb/C, but no C57Bl/6, mice developed AHR, tissue eosinophilia, and increased local IL-4 and IL-5 production. Functional changes peaked at day 4 and 7, after the larva had left the lungs. AHR was clearly dependent on IL-4 but not on eosinophils, as evaluated by experiments in IL-4 and Gata-1-deficient mice. Experiments in ST2-deficient mice showed that this pathway was not needed for induction of AHR but was necessary for the maintenance of AHR and for Th2 responses in the lung. These studies clearly show a crucial role for IL-4 in the induction of AHR following S. venezuelensis infection and for IL-33/ST2 in maintaining AHR and lung Th2 responses.

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Role of IL-13 in a model of Strongyloides venezuelensis infection in rats

Cited by 4 publications

References 21 publications

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Avaliação parasitológica e sorológica de coinfecção por Syphacia muris e Strongyloides venezuelensis

The role of IL-33/ST2, IL-4, and eosinophils on the airway hyperresponsiveness induced by Strongyloides venezuelensis in BALB/c mice

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