2018
DOI: 10.4168/aair.2018.10.3.260
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Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Abstract: PurposeMolecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism.MethodsThe effects of different concentrations of IL-13 on the prolifera… Show more

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Cited by 19 publications
(11 citation statements)
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“…From multitudinous candidate miRNAs, we selected nine miRNAs for further analysis since these miRNAs had been previously reported to be able to regulate the phenotype of asthmatic ASMCs. For example, miR-20b inhibits PDGF-induced human fetal ASMC proliferation by targeting STAT3; miR-155 inhibits IL-13-induced human ASMC proliferation and migration by targeting TGF-β-activated kinase 1/MAP3K7-binding protein 2; and miR-150 and miR-135a, upregulated by Schisandrin, inhibits ASMC proliferation and migration in asthmatic rats (Kuhn et al, 2010; Bartel et al, 2018; Shi et al, 2018). Next, we performed a series of experiments and found that in these miRNAs, Malat1 only could directly target miR-150, and regulate miR-150 expression.…”
Section: Discussionmentioning
confidence: 99%
“…From multitudinous candidate miRNAs, we selected nine miRNAs for further analysis since these miRNAs had been previously reported to be able to regulate the phenotype of asthmatic ASMCs. For example, miR-20b inhibits PDGF-induced human fetal ASMC proliferation by targeting STAT3; miR-155 inhibits IL-13-induced human ASMC proliferation and migration by targeting TGF-β-activated kinase 1/MAP3K7-binding protein 2; and miR-150 and miR-135a, upregulated by Schisandrin, inhibits ASMC proliferation and migration in asthmatic rats (Kuhn et al, 2010; Bartel et al, 2018; Shi et al, 2018). Next, we performed a series of experiments and found that in these miRNAs, Malat1 only could directly target miR-150, and regulate miR-150 expression.…”
Section: Discussionmentioning
confidence: 99%
“…Based on previous studies, it is recognized that abnormal expression profiles of miRNAs are closely related to the development of arteriosclerosis [9,15], and VSMC proliferation and migration occur at every stage of arteriosclerosis [7,16]. Recently, many studies have shown that miRNAs play a critical role in the regulation and control of VSMC proliferation and migration [17][18][19]. Here, miR-125a-3p was observed to be significantly reduced in arteriosclerosis and restenotic arteries compared with that of the control group.…”
Section: Discussionmentioning
confidence: 82%
“…Lower chambers containing media with 0.1% FBS were included to serve as negative controls. Following a 6 hour incubation period, non-migrated cells (upper chamber) were removed with a sterile cotton swab 67 . Media was removed from the lower chamber followed by addition of 500 µL of fresh media and 100 µl of MTS solution and incubated for 4 hours followed by measurement of absorbance at 490 nm through microplate reader (Molecular Devices, California, USA) 68 .…”
Section: Methodsmentioning
confidence: 99%