Role of cell block preparation in neoplastic lesions

Basnet, Sahisnuta; Talwar, OP

doi:10.3126/jpn.v2i4.6876

Cited by 26 publications

(26 citation statements)

References 13 publications

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“…This study correlates with the study of Basnet et al, [11] where diagnostic accuracy was 95.91% and also with the study of Rajib et al, [12] who did a study on Cell blocks on thyroid cytology where diagnostic accuracy was found to be 95.7%.…”

Section: Percentage (%)supporting

confidence: 91%

“…Cell blocks provided more additional information like we could appreciate better cytological details in cell blocks as cases like papillary carcinoma where Psammoma bodies [ Fig-10], Orphan Annie eye nucleus and papillary structures could be well appreciated which cannot be visualized in FNAC, so Cell Blocks were more contributory. In a study by Basnet et al, [11] stated that Cell Blocks were more contributory in diagnosis than that of Direct smears. Rajib et al, [12] and Kathrine et al [19] also stated that Cell Blocks provide additional valuable information in most cases.…”

Section: Cell Block Findingsmentioning

confidence: 98%

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Diagnostic Utility of Cell Blocks in Thyroid Aspirates

Ahmed¹,

Medhi²,

Das³

2015

jemds

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BACKGROUND: Thyroid swellings are a common clinical presentation. The distribution of benign lesions from malignant lesions cannot be made by clinical judgment. The pathological lesions need morphological workup, Fine Needle Aspirations (FNA) being one of the cornerstones. Its limitations are sample inadequacy, low cytomorphologic details and architectural insights with overlapping of cytological features. Cell block is a diagnostic method where material for morphologic evaluation is well recognized as it increases cellular yield, thus improves diagnostic accuracy. It provides excellent cytomorphologic details in concert with architectural insight with much more diagnostic efficacy, thus obviates repeat FNA. AIMS: To analyze the diagnostic utility of cell blocks on thyroid aspirates by taking the histopathological findings as the gold standard and statistically analyzed for diagnostic efficacy of cell block. MATERIALS AND METHOD: The study design was hospital based cross-sectional study. Ninety FNAC samples were taken and cell block was prepared using Tissue Thromboplastic method. Cell Block findings were analyzed by looking at the arrangement of follicular cells size, acini, nuclear cytoplasmic characteristics, colloid, stroma, inflammatory cells, malignant cells, psammoma bodies, haemorrhage, necrosis, calcification, fibrosis, amyloid or any other relevant findings. RESULTS: Out of the 90 cases, 69 cases (76.67%) were diagnosed as Non neoplastic lesions and 21cases (23.33%) as Neoplastic. Of the Non-neoplastic cases, colloid goiter was the commonest, (64.44%). Among the Neoplastic lesions Follicular adenoma was the commonest (11.11%). Histopathologically, out of the 47 cases, Non-neoplastic lesions were 32 cases (68.09%) and neoplastic lesions 15 cases (31.91%). Colloid goiter was the commonest thyroid lesion (53.19%). In Neoplastic lesions Follicular adenoma cases was the commonest (21.28%).There was 0 False Negative case and 2 cases of False Positive in our series. CONCLUSION: Cell block technique showed to be highly effective with high sensitivity and specificity and could be utilized as a cost effective diagnostic tool in the armamentarium of diagnostic work up in the patients with thyroid lesions.

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Section: Percentage (%)supporting

confidence: 91%

Section: Cell Block Findingsmentioning

confidence: 98%

Diagnostic Utility of Cell Blocks in Thyroid Aspirates

Ahmed¹,

Medhi²,

Das³

2015

jemds

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“…The specificity was increased to 93.33% with combined 1 In the cell blocks study of various organs, Basnet et al found were cell block technique superior in diagnosing neoplasm than smears with diagnostic accuracy of 95.91% and 91.8% respectively. 8 In the cell block study of 46 cases of image-guided fine needle aspiration cytology of abdomino-pelvic and intrathoracic masses by Methew et al, the sensitivity, specificity, and diagnostic accuracy of cell blocks was 71.11%, 100%, and 71.73% respectively. The figures for FNA smears were 62.22%, 100%, and 63.04%, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…2 Various methods for preparing paraffin-embedded cell blocks from FNAC have been reported, which include direct transfer of all centrifuged cellular material wrapped in lens paper, filter paper or embedding in plasma thrombin or agar and then processing as a routine histological specimen. 8 In this study cell block preparation the cellular material was wrapped in whatman filter paper for further histological processing and embedded in the paraffine block. The reagent cost for making the cell blocks by this method is very less as compared to reagent cost for making the cell block by plasma thrombin method and can be done in any modest laboratory.…”

Section: Discussionmentioning

confidence: 99%

Role of Cell Block Preparation in Cytopathological Diagnosis

Parate¹,

Pawar²,

Kakade³

2019

IJCMR

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Introduction: Though Fine needle Aspiration Cytology (FNAC) is good method for diagnosis of lesions, sometimes it does not provide precise diagnosis due to inadequate material. Present study was based on use of residual FNAC material to make cell block in order to find out utility of cell block preparation of FNAC. Material and Methods:The two years prospective study carried out in a tertiary Heath care hospital of central India, included 360 patients referred for FNAC of the lesions from all body sites. After making the conventional FNAC smears, the remaining material in the needle hub and syringe was obtained by flushing it with 10% alcohol -formalin. After centrifugation the supernatant was decanted and deposit fixed in freshly prepared 10% alcohol-formalin. After half an hour alcohol-formalin was drained, cell button was taken on whatman-filter paper and processed as surgical tissue in automated tissue processing machine. FNAC smears and cell block sections were examined separately for cellularity, reporting done and results correlated with histopathology.Results: Of 360 cases FNAC smears and cell blocks were adequate in 324(90%) and 313(86.94%) cases respectively. Statastical analysis was done in 98 cases in which histopathology available. Sensitivity of FNAC was 88.06%,specificity of 92.86% and accuracy of 89.47%. Sensitivity of cell block was 90.91%, specificity of 92.86% and accuracy of 91.47%. Sensitivity of combined FNAC and cell block technique was increased to 98.53% with specificity of 93.33% and accuracy of 96.94%. Conclusion:Cell blocks as an adjuvant to FNAC smears is very effective technique in cytology to reach the definitive diagnosis.

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“…[6][7] Although open surgical biopsy is the 'gold standard' for diagnosis of palpable breast lesions, two types of minimally invasive breast biopsy techniques, core needle biopsy (CNB) and fine needle aspiration cytology (FNAC), have become established for the diagnostic evaluation of palpable breast lesions in the recent years. 8 Core biopsy is also an effective mean to diagnose breast lumps, but it is time consuming and expensive, and can be associated with complications like haematoma and rarely pneumothorax. [9][10] Adnan K et al, described some of the difficulties and pitfalls in FNAC such as inadequate samples, suspicious malignancies and overlapping features of different lesions.…”

Section: Self-breast Examination Along With Triple Test Which Includementioning

confidence: 99%

Correlation of fine needle aspiration cytology with histopathological diagnosis in assessing breast lumps at a tertiary care hospital

George¹,

Antony²

2018

Int J Res Med Sci

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Background: Breast cancer is one of the most common malignancies in women worldwide (22%) and India ranks the second after cervical cancer. The diagnostic accuracy of FNAC increases to 99% when it is combined with clinical and radiological examination. In this study, authors plan to correlate the cytological findings with histopathological examinations for breast lesions and determine the accuracy of fine needle aspiration cytology in the diagnosis of breast lesions.Methods: It is a retrospective study carried out in the Department of Pathology at a Tertiary Care Hospital. All the FNAC results of breast lesions during the one year period were collected. Gauge needle maximum of 3 passes were made and the slides were fixed in 70-80% alcohol and stained with routine haematoxylin and eosin stain.Results: Among 200 patients, 197 were females and 3 were males. Benign breast lesions were found in 158 cases; among which fibroadenoma was the commonest lesion. Malignancy was observed in 25 cases. Two cases of phyllodes tumour were incorrectly reported as fibroadenoma on cytology. Of 12 cases which were diagnosed to have atypical lesions, 4 cases were papillary neoplasm, and 8 cases were atypical ductal hyperplasia.Conclusions: This study concludes that breast FNAC is a reliable, easy, cheap and effective procedure for the diagnosis. It reduces the need of core needle biopsies and very well correlated with histopathological examination. FNAC differentiates non neoplastic from the neoplastic by which it reduces the patient’s anxiety and helps the surgeons in planning the mode of treatment.

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Role of cell block preparation in neoplastic lesions

Cited by 26 publications

References 13 publications

Diagnostic Utility of Cell Blocks in Thyroid Aspirates

Diagnostic Utility of Cell Blocks in Thyroid Aspirates

Role of Cell Block Preparation in Cytopathological Diagnosis

Correlation of fine needle aspiration cytology with histopathological diagnosis in assessing breast lumps at a tertiary care hospital

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