Role of biomaterials, therapeutic molecules and cells for hepatic tissue engineering

Kirthanashri, S. V.; Subramanian, Anuradha; Krishnan, Uma Maheswari; Sethuraman, Swaminathan

doi:10.1016/j.biotechadv.2012.01.004

Cited by 57 publications

(33 citation statements)

References 112 publications

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“…As shown in Table 1, the content of galactose units in the LCC macromolecule was 3.30%. The contents of lignin and total sugars were 25.5% and 64.62%, respectively, which gave the spherical biocarriers good physical strength [37]. The results suggest that spherical biocarriers are suitable for use as biocarriers of human hepatocytes.…”

Section: Composition Analysismentioning

confidence: 85%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m 2 g −1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0∼9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes.

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Section: Composition Analysismentioning

confidence: 85%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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“…Scaffolds blended with various materials have been constructed for the repair of different tissues, such as bones, liver and other organs [1,2,5–7]. However, attempts to construct scaffolds for the repair of the central nervous system (CNS) have had limited success, because of its intrinsic complexity, low regenerative potential and anatomically restrictive nature, which pose a unique set of challenges [8–13].…”

Section: Introductionmentioning

confidence: 99%

Adhesion to Carbon Nanotube Conductive Scaffolds Forces Action-Potential Appearance in Immature Rat Spinal Neurons

et al. 2013

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In the last decade, carbon nanotube growth substrates have been used to investigate neurons and neuronal networks formation in vitro when guided by artificial nano-scaled cues. Besides, nanotube-based interfaces are being developed, such as prosthesis for monitoring brain activity. We recently described how carbon nanotube substrates alter the electrophysiological and synaptic responses of hippocampal neurons in culture. This observation highlighted the exceptional ability of this material in interfering with nerve tissue growth. Here we test the hypothesis that carbon nanotube scaffolds promote the development of immature neurons isolated from the neonatal rat spinal cord, and maintained in vitro. To address this issue we performed electrophysiological studies associated to gene expression analysis. Our results indicate that spinal neurons plated on electro-conductive carbon nanotubes show a facilitated development. Spinal neurons anticipate the expression of functional markers of maturation, such as the generation of voltage dependent currents or action potentials. These changes are accompanied by a selective modulation of gene expression, involving neuronal and non-neuronal components. Our microarray experiments suggest that carbon nanotube platforms trigger reparative activities involving microglia, in the absence of reactive gliosis. Hence, future tissue scaffolds blended with conductive nanotubes may be exploited to promote cell differentiation and reparative pathways in neural regeneration strategies.

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“…Unfortunately, these polymers exhibit some drawbacks, such as poor cell affinity and cell-matrix interaction. In addition, polymers will degrade and release acidic degradation products, which are likely to trigger aseptic inflammation reaction and swelling [98,99]. Cao et al [100] fabricated three-dimensional porous composite scaffolds of PGA/β-TCP (in 1:1 and 1:3 weight ratios) by a combination of the solvent casting and particulate leaching method.…”

Section: Ceramic-based Composite Scaffoldsmentioning

confidence: 99%

Current Progress in Bioactive Ceramic Scaffolds for Bone Repair and Regeneration

Gao

Deng

Feng

et al. 2014

IJMS

262

143

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Bioactive ceramics have received great attention in the past decades owing to their success in stimulating cell proliferation, differentiation and bone tissue regeneration. They can react and form chemical bonds with cells and tissues in human body. This paper provides a comprehensive review of the application of bioactive ceramics for bone repair and regeneration. The review systematically summarizes the types and characters of bioactive ceramics, the fabrication methods for nanostructure and hierarchically porous structure, typical toughness methods for ceramic scaffold and corresponding mechanisms such as fiber toughness, whisker toughness and particle toughness. Moreover, greater insights into the mechanisms of interaction between ceramics and cells are provided, as well as the development of ceramic-based composite materials. The development and challenges of bioactive ceramics are also discussed from the perspective of bone repair and regeneration.

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Role of biomaterials, therapeutic molecules and cells for hepatic tissue engineering

Cited by 57 publications

References 112 publications

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Adhesion to Carbon Nanotube Conductive Scaffolds Forces Action-Potential Appearance in Immature Rat Spinal Neurons

Current Progress in Bioactive Ceramic Scaffolds for Bone Repair and Regeneration

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