Role of air and Light in Sclerotial Development and Basidiospore Formation in Sclerotium Rolfsii

Maurya, Sudarshan; Singh, Rashmi; Singh, Amitabh; Singh, Harikesh Bahadur

doi:10.2478/v10045-010-0035-z

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…After this incubation period, sclerotial formation and production were determined. Sclerotial formation was noted every 3 days [26]. Sclerotial production was determined by removing mature sclerotia with a sharp scalpel.…”

Section: Effect Of Temperature On Pathogen Mycelial Growth and Scleromentioning

confidence: 99%

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Ayed¹,

Jabnoun‐Khiareddine²,

Abdallah³

et al. 2018

J Plant Pathol Microbiol

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Sclerotium rolfsii is a serious soilborne phytopathogenic fungus causing serious yield loss in crops of high economic importance. In this study, the effects of temperatures and culture media were evaluated on S. rolfsii mycelial growth, sclerotial production and germination. The three isolates tested grew over a range of temperatures from 10°C to 35°C but not at 5°C and 40°C. Radial growth and dry mycelium production were highest at 30°C. Sclerotial initiation started on the 3 rd day after incubation at 30°C and 35°C, and mature sclerotia were observed after 15 and 6 days of incubation, respectively. Optimal sclerotial production and the dry weight of 100 sclerotia varied depending on isolates and temperatures and occurred at 25°C-35°C. No mature sclerotia were produced at 5°C, 10°C, 15°C and 40°C. Sclerotial germination, noted after 24 h of incubation, was 96%-100% at 25°C-35°C, declined at 15°C-20°C, and was totally inhibited at 5°C, 10°C and 40°C even after 72 h of incubation. For all S. rolfsii isolates, optimal radial growth occurred on OAT medium followed by CZA, MYEA and PDA. When grown on PDA and OAT media, S. rolfsii developed cottony colonies with abundant mycelium whereas on WA and CZA pathogen growth was thin and scanty. Colonies formed on the other media developed sparse and flat mycelia. For sclerotial formation, WA and CZA were the most suitable culture media but no dark brown sclerotia were observed on NA and YDA. The highest sclerotial formation was observed on PDA and CZA. The highest sclerotium weight was produced by Sr 3 isolate on PDA whereas for Sr 1 and Sr 2 isolates, sclerotial weights were significantly comparable on PDA, MYEA and CZA. Sclerotial germination was optimal on all tested media except NA. The significance of these findings on S. rolfsii biology is discussed.

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Section: Effect Of Temperature On Pathogen Mycelial Growth and Scleromentioning

confidence: 99%

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Ayed¹,

Jabnoun‐Khiareddine²,

Abdallah³

et al. 2018

J Plant Pathol Microbiol

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“…The methodology used in this research was adapted and modified based on previous techniques used for the study of smut fungi as mentioned by Fernández et al (1978), Matyac (1985), Craig and Frederiksen (1992), Maurya et al (2010) and Kosiada (2011).…”

Section: Methodsmentioning

confidence: 99%

Biology and histopathology of Ustilago filiformis (=U. longissima), a causal agent of leaf stripe smut of Glyceria multiflora

Perelló¹,

Gassó²,

Lovisolo³

2015

Journal of Plant Protection Research

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Abstract:The aims of this study were to clarify the reproductive biology of the Ustilago filiformis Schrank, as causal agent of the stripe smut of Glyceria multiflora, determine the infection process of the pathogen and analyze the histological changes associated hostGlyceria any fungus attack. Moreover, the life cycle of the fungus was elucidated for the first time. Both teliospores and basidiospores were found to be equally efficient in producing the infection in Glyceria plants after the plants had been inoculated. These results constitute an important contribution for the understanding of the epidemiology of the disease.

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“…The fungus can survive in the form of sclerotia or hyphae in the soil or remain on infected plants (Soenartiningsih et al 2015). Various biotic and abiotic factors directly or indirectly influence the development of sclerotia (Maurya et al 2010). The vegetative growth and morphogenesis of fungi, such as hyphal development, sporulation, and sclerotial formation, are affected by nutrients and physical factors, such as temperature, water or humidity, pH and light (Moromizato et al 1983).…”

Section: Introductionmentioning

confidence: 99%

Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia

Budiarti

Lukman²,

Sumardiyono

et al. 2019

Biodiversitas

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Abstract. Budiarti SW, Lukman R, Sumardiyono C, Wibowo A, Priyatmojo A. 2019. Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia. Biodiversitas 20: 2028-2038. Banded leaf and sheath blight (BLSB) caused by Rhizoctonia solani Kuhn is a significant constraint of maize production in Indonesia, especially within the last several years. R. solani is an important soil-borne necrotrophic fungal pathogen which is known to be sensitive to different environmental conditions. The differential response of fungi to light and dark is important to study, especially of those causing disease to crop plant as it helps to reveal the factors regulating the pathogenicity of the fungus. This study aims to determine the effect of light duration on the mycelial development and sclerotia formation of R. solani isolates obtained from different locations in Yogyakarta Special Region and Central Java, Indonesia. Ten isolates of R. solani collected from different geographic regions in Yogyakarta Special Region, and Central Java from maize (nine isolates) and rice (one isolate) were exposed to three different light conditions: 12 h alternating light/dark, continuous darkness, and continuous light. Cultural morphology and sclerotial characteristics were recorded for each isolate. All isolates revealed great diversity in terms of cultural and morphological characteristics after exposure to three different light conditions. Under complete darkness, the mycelial growth phase was extended, and a fluffy mass of mycelia was produced. All isolates showed abundant growth under the 12 h alternating light/dark condition than under the continuous dark and continuous light conditions. Sclerotia were initiated earlier under the 12 h alternating light/dark condition than under other conditions. The 12 h alternating light/dark condition also induced number and heavier weight of sclerotia compared with those produced under the continuous light and continuous dark conditions. The modifications in light condition exert a significant impact on some R. solani isolates.

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Role of air and Light in Sclerotial Development and Basidiospore Formation in Sclerotium Rolfsii

Cited by 9 publications

References 14 publications

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination

Biology and histopathology of Ustilago filiformis (=U. longissima), a causal agent of leaf stripe smut of Glyceria multiflora

Effect of photoperiod on the cultural morphology of Rhizoctonia solani isolates of maize from Yogyakarta and Central Java, Indonesia

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