Role of Aeromonas Isolated from Feces of Finnish Patients

Rautelin, Hilpi; Sivonen, Aulikki; Kuikka, Arja; Ov, Renkonen; Valtonen,; H, Lehti; Kahanpää, Asko; Tu, Kosunen

doi:10.3109/00365549509019010

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…Specifically, Aeromonas spp. were isolated from 1.4% of subjects with diarrhea (n, 978) in one study (12) and 1.9% of subjects (n, 13,027) in another study (21). In 343 asymptomatic subjects in the latter study, no Aeromonas sp.…”

Section: Discussionmentioning

confidence: 88%

Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis

Riley

Berger²,

Miner³

et al. 1998

J Clin Microbiol

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Treatment of chronic prostatitis/chronic pelvic pain syndrome is often empirical because clinical culture methods fail to detect prostate-associated pathogens in >90% of patients. Previously, we tested a variety of specific-microorganism PCRs and began a DNA sequence study after we found that 77% of prostatitis patients were PCR positive for prokaryotic rRNA-encoding DNA sequences (rDNAs) despite negative cultures using optimal techniques. In the present study, 36 rDNA clones from 23 rDNA-positive patients were sequenced. This study represents more than twice the total rDNA sequence and more than twice the number of patients in the previous study. The increased number of patients and clones sequenced allowed enhanced phylogenetic analyses and refinements in our view of rDNA species inhabiting the prostate. A continuum of related rDNAs that might be arbitrarily described as two major groups of rDNAs and several minor groups was found. Sequences termed Pros A, identified in 8 (35%) of 23 rDNA-positive patients, grouped withAeromonas spp. in phylogenetic studies. Sequences termed Pros B, identified in 17 (74%) of 23 rDNA-positive patients, were distinct from previously reported sequences, although all were >90% similar to known gram-negative bacteria. Of the nine patients for whom multiple rDNAs were sequenced, six had biopsy specimens containing rDNAs from more than one species. Four (17%) patients had rDNAs different from those of the Pros A and Pros B groups. Of these four, one patient had rDNA similar to that ofFlavobacterium spp., another had rDNA similar to that of Pseudomonas testosteroni, and two patients had rDNAs <70% similar to known rDNAs. These findings suggest that the prostate can harbor bacteria undetectable by traditional approaches. Most of these diverse sequences are not reported in environments outside the prostate. The sequence similarities suggest adaptation of limited groups of bacteria to the microenvironment of the prostate. Further studies may elucidate the relationship of prostate-associated bacteria to chronic prostatitis/chronic pelvic pain syndrome.

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Section: Discussionmentioning

confidence: 88%

Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis

Riley

Berger²,

Miner³

et al. 1998

J Clin Microbiol

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“…Aeromonas species are ubiquitous in the freshwater environment and are important human and fish pathogens (10,19,45). Aeromonas hydrophila, Aeromonas veronii biovar sobria, and Aeromonas caviae have been implicated in causing septicemia, wound infections, and diarrhea in humans (7,19,20,34). The prevalence of diarrhea appears to be especially high in children under the age of 2 years (6,15).…”

mentioning

confidence: 99%

Symbiosis of Aeromonas veronii Biovar sobria and Hirudo medicinalis , the Medicinal Leech: a Novel Model for Digestive Tract Associations

Graf

1999

Infect Immun

114

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Hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. Infections by Aeromonasoccur in up to 20% of patients unless a preemptive antibiotic treatment is administered. The associated infections demonstrate the need for a better understanding of the digestive tract flora ofH. medicinalis. Early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of blood. In this study, we cultivated bacteria from the digestive tract and characterized them biochemically. The biochemical test results identified the isolates as Aeromonas veronii biovar sobria. This species identification was supported by sequence comparison of a variable region of the genes coding for 16S rRNA. In a colonization assay, a rifampin-resistant derivative of a symbiotic isolate was fed in a blood meal to H. medicinalis. The strain colonized the digestive tract rapidly and reached a concentration similar to that of the native bacterial flora. For the first 12 h, the in vivo doubling time was 1.2 h at 23°C. After 12 h, at a density of 5 × 107 CFU/ml, the increase in viable counts ceased, suggesting a dramatic reduction in the bacterial growth rate. Two human fecal isolates, identified as Aeromonas hydrophila and A. veronii biovar sobria, were also able to colonize the digestive tract. These data demonstrate that the main culturable bacterium in the crop of H. medicinalis isA. veronii biovar sobria and that the medicinal leech can be used as a model for digestive tract association ofAeromonas species.

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“…Aeromonas species are gram-negative, oxidase-positive rods that have been associated with diarrheal disease. They have been found with a frequency of 2% or higher in children with diarrhea but not in asymptomatic controls [17,18]. Infection is more common in warmer months.…”

Section: Bacterial Enteritismentioning

confidence: 94%

“…Infection is more common in warmer months. The diarrhea is usually watery and self-limited, although bloody stools and persistent diarrhea may occur [17]. At least one human isolate of Aeromonas hydrophila has demonstrated a high degree of cytotoxicity [19•].…”

Section: Bacterial Enteritismentioning

confidence: 99%

New causes and treatments for infectious diarrhea in children

Rudolph

Cohen

1999

Curr Gastroenterol Rep

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Infectious diarrhea is a universal and important health problem in the pediatric population. An expanding number of potential viral, bacterial, and parasitic pathogens have been associated with diarrheal disease. However, the epidemiologic association of a microorganism with diarrhea is only one step in the process of identifying new pathogens. Once the virulence mechanisms of these organisms are elucidated, a causal relationship can be more readily defined. This article reviews the etiologic agents of diarrhea in the pediatric population and focuses on the newer treatment and prevention modalities, including probiotics and vaccinations, which are used increasingly to combat these diseases.

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Role of Aeromonas Isolated from Feces of Finnish Patients

Cited by 9 publications

References 14 publications

Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis

Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis

Symbiosis of Aeromonas veronii Biovar sobria and Hirudo medicinalis , the Medicinal Leech: a Novel Model for Digestive Tract Associations

New causes and treatments for infectious diarrhea in children

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