Rocket immunoelectrophoresis (RIE) for determination of potentially allergenic peanut proteins in processed foods as a simple means for quality assurance and food safety

Holzhauser, Thomas; Dehne, Lutz Ingo; Hoffmann, Andreas; Haustein, Dieter; Vieths, Stefan

doi:10.1007/s002170050203

Cited by 23 publications

(12 citation statements)

References 1 publication

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“…In RIE, an antibodycontaining gel is used to form antigen-antibody precipitates as allergens migrate through the gel (Poms et al 2004). This method is losing favorability owing to laborious gel-related processes and difficult staining protocols (Holzhauser et al 1998, Poms et al 2004). …”

Section: Miscellaneous Methodsmentioning

confidence: 99%

Food Allergy

Sathe

Liu

Zaffran

2016

Annu. Rev. Food Sci. Technol.

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Food allergy is receiving increased attention in recent years. Because there is currently no known cure for food allergy, avoiding the offending food is the best defense for sensitive individuals. Type I food allergy is mediated by food proteins, and thus, theoretically, any food protein is a potential allergen. Variability of an individual's immune system further complicates attempts to understand allergen-antibody interaction. In this article, we briefly review food allergy occurrence, prevalence, mechanisms, and detection. Efforts aimed at reducing/eliminating allergens through food processing are discussed. Future research needs are addressed.

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Section: Miscellaneous Methodsmentioning

confidence: 99%

Food Allergy

Sathe

Liu

Zaffran

2016

Annu. Rev. Food Sci. Technol.

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“…1 Developing "allergen" and "protein" immunoassays for determination of allergenic proteins in food (example for peanuts is given). Pabpolyclonal antibodies, mab -monoclonal antibodies and RIE have all been found suitable for separating allergenic proteins [14,16,17,18]. Among these, SDS-PAGE is most frequently used.…”

Section: Immunoanalytical Techniquesmentioning

confidence: 98%

Immunoanalytical detection of allergenic proteins in food

Krska¹,

Welzig²,

Baumgartner³

2004

Analytical and Bioanalytical Chemistry

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“…The height of the rocket increases using matrixdependent standard curves. This effect makes it possible to further increase the sensitivity of the method (Holzhauser et al 1998). However, this has not been attempted in the present study.…”

Section: Discussionmentioning

confidence: 96%

“…However, this has not been attempted in the present study. The importance of using standard curves in the representive food matrix has earlier been demonstrated for ELISA (Yeung & Collins 1996) and RIE (Holzhauser et al 1998).…”

Section: Discussionmentioning

confidence: 98%

Rocket immunoelectrophoresis and ELISA as complementary methods for the detection of casein in foods?

Moen

Sletten

Miller

et al. 2005

Food and Agricultural Immunology

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A rocket immunoelectrophoresis (RIE) method is described and compared with a commercial ELISA kit. Thirteen food products were selected from the categories sausage, rice pudding, fruit-ice, biscuit and baby-dinner. These had all tested negative for casein in a previous investigation using an older commercial ELISA kit. In the present study, using a more recent commercially developed ELISA kit and RIE, only three of the 13 products still tested negative for casein. However, the RIE detected levels of casein in ten of the remaining products, whereas the ELISA revealed the presence of casein only in the three products giving the highest levels of casein with RIE. Thus, the two methods both confirm the presence of casein when the levels in the samples are within the range of the detection limit for the ELISA method.

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Rocket immunoelectrophoresis (RIE) for determination of potentially allergenic peanut proteins in processed foods as a simple means for quality assurance and food safety

Cited by 23 publications

References 1 publication

Food Allergy

Food Allergy

Immunoanalytical detection of allergenic proteins in food

Rocket immunoelectrophoresis and ELISA as complementary methods for the detection of casein in foods?

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