Immunoanalytical detection of allergenic proteins in food

Krska, Rudolf; Welzig, E.; Baumgartner, Sabine

doi:10.1007/s00216-003-2311-5

Cited by 25 publications

(18 citation statements)

References 23 publications

(27 reference statements)

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“…Besides, allergy to cow's milk proteins can be a precursor to other, and sometimes, more serious diseases, such as other food allergies, respiratory allergies or, in the worst cases, even death [1,3].…”

Section: Introductionmentioning

confidence: 99%

Analysis of trace amounts of bovine β‐lactoglobulin in infant formulas by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

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Bovine β‐lactoglobulin (βLG) has been described by several authors as the main allergen present in cow's milk. It can induce allergic reactions even at the low concentration existing in hypoallergenic formulas based on hydrolyzed cow's milk proteins (generally lower than μM). In this paper, the usefulness of a capillary electrophoresis method with on‐capillary derivatization and laser‐induced fluorescence detection for the analysis of trace amounts of βLG in a commercial hypoallergenic formula has been demonstrated. To confirm the identity of the peak of βLG based on migration time, an immunorecognition step employing an anti‐βLG antibody was performed. βLG was quantitated in the whey and casein fractions of the hypoallergenic formula. The concentration of βLG in the whey fraction of the formula was about 3 orders of magnitude lower than the average value present in cow's milk. In the casein fraction of the formula, the concentration of βLG was about 1 order of magnitude lower than in the whey fraction. The method developed was also used for the quality control of three cereal‐based infant foods formulated without milk to test the presence or absence of βLG as an indicator of milk contamination during the fabrication process. βLG in a concentration of 10–7 M or higher was not observed in any of the cereal‐based infant formulas analyzed.

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Section: Introductionmentioning

confidence: 99%

Analysis of trace amounts of bovine β‐lactoglobulin in infant formulas by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

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“…26 However, a general guideline for the detection of food allergens has been reported as 1-100 mg/kg of food. 27 Assuming food has the same density as water, this yields an allergenic threshold of 1-100 µg/mL. The detection limit reported here is well below this range.…”

Section: Resultsmentioning

confidence: 64%

Impedance Biosensor Utilizing a Si Substrate Deposited by Wet Methods

Falola¹,

Radhakrishnan²,

Suni³

2015

ECS Electrochemistry Letters

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We report an impedance biosensor utilizing a Si electrode created by wet chemical deposition atop 6061 Al alloy. The sensor electrode is created by galvanic/electroless Si deposition from an electrolyte containing 10 mM HF and 20 mM Na2SiF6 in 80 wt% formic acid, followed by antibody immobilization. The impedance response of the sensor electrode to increasing concentrations of peanut protein Ara h 1, a common food allergen, can be fit to an equivalent circuit containing three RC loops. The circuit element most sensitive to antigen binding is the charge transfer resistance, yielding a detection limit of 4 ng/mL.Biosensors that utilize electrochemical impedance spectroscopy have been employed with a wide variety of immobilized biomolecules, including antibodies, receptor proteins, aptamers, and ssDNA.

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“…cell adhesion [64], innate immunity [3], CO 2 assimilation in metabolic pathways [30], extracellular matrix degradation [2] and membrane construction [17,44,85]) as well as the binding properties of retrocyclins [14], apotosis regulators [42], antimicrobial peptides [23], glycosphingolipids [97], barley -amylase/subtilisin inhibitor [66], amelogenin [67], lysenin [45], lectins [65], endoxylases [40], connexins [21], selectins [25], phosphoinositides [71], carbohydrates [20,70,72], HIV proteins [48,91], tumor necrosis factor receptor superfamily [95] and the insulin-like growth factor system [58]. Other reviews highlight the integration of optical biosensors with complementary technologies in areas of applied research, including drug development and chemical genetics [1,50,73,83,88], plant proteomics [33], gene and protein expression [29,35,86], antibody optimization [5,46,62], food industry [4,47,54,61,79], bioprocessing and manufacturing [12,…”

Section: Reviews Theory and Methodsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

114

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The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

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Immunoanalytical detection of allergenic proteins in food

Cited by 25 publications

References 23 publications

Analysis of trace amounts of bovine β‐lactoglobulin in infant formulas by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Analysis of trace amounts of bovine β‐lactoglobulin in infant formulas by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Impedance Biosensor Utilizing a Si Substrate Deposited by Wet Methods

Survey of the year 2004 commercial optical biosensor literature

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